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SNP molecular marker influencing cashmere fine character of cashmere goat and application of SNP molecular marker

A molecular marker, cashmere goat technology, applied in the field of animal genetics and breeding, can solve the problems that are not conducive to the excellent cashmere production traits of cashmere goats, reduce the economic benefits of breeding cashmere goats, slow down the breeding and improvement of cashmere goats, etc., so as to improve international competitiveness and improve The effect of breeding efficiency and saving breeding cost

Pending Publication Date: 2021-11-12
INNER MONGOLIA AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the thickening of cashmere will affect the quality of yarn, reduce the quality of textiles, and then reduce the economic benefits of breeding cashmere goats, and it is not conducive to the maintenance of excellent cashmere production traits of cashmere goats, which will slow down the breeding and improvement of cashmere goats

Method used

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  • SNP molecular marker influencing cashmere fine character of cashmere goat and application of SNP molecular marker
  • SNP molecular marker influencing cashmere fine character of cashmere goat and application of SNP molecular marker
  • SNP molecular marker influencing cashmere fine character of cashmere goat and application of SNP molecular marker

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Experimental program
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Embodiment Construction

[0030] 1 Experimental materials

[0031] 1.1 Equipment and reagents

[0032] Reagents: petroleum ether, 75% alcohol, 10% SDS, proteinase K, STE, saturated Tris phenol, DNA extraction solution 25:24:1 phenol: chloroform: isoamyl alcohol, ice isopropanol, enzyme-free water, agarose, TAE, nucleic acid dye, DL500 DNA Maker, etc.

[0033] Equipment: Auricular forceps, surgical scissors, pipette gun, parafilm, OFDA fiber fineness tester, constant temperature culture shaker, centrifuge, NanoDrop 2000C ultra-micro spectrophotometer, ABI Veriti gradient PCR instrument, electronic balance, DYY -6C bistable electrophoresis instrument power supply, electrophoresis tank, Bio-Rad ChemiDoc XRS+imager, etc.

[0034] 1.2 Sample collection

[0035] Select 1,920 Inner Mongolia cashmere goats from Inner Mongolia Yiwei Cashmere Goat Co., Ltd., use surgical scissors to collect villi samples behind the scapula on the abdominal midline of individual cashmere goats, put them into plastic bags, and ...

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Abstract

The invention discloses an SNP molecular marker for influencing the cashmere fine character of cashmere goats and application of the SNP molecular marker. The SNP molecular marker is a G-to-A mutation at a 28342240bp position on a chromosome 8 of a goat three-generation genome ARS1 version; the SNP molecular marker is located on a CCDC171 gene; the nucleotide sequence of the SNP molecular marker is as shown in SEQ ID NO: 1, and M in the nucleotide sequence represents a base mutation site and is selected from a base G or A. By applying the molecular marker to the breeding and identification of the fine cashmere character variety of the down producing goat, the fine cashmere character of the down producing goat can be improved, the quality of cashmere produced by the bred down producing goat is higher, and greater economic value is obtained; when the method is used for breeding the fine cashmere goat strain and the genetic improvement method for the fine cashmere character of the cashmere goat, the cashmere goat strain with higher economic value can be obtained, so that the fine cashmere dominant character of the whole cashmere goat species is improved, and the method has immeasurable economic and breeding values.

Description

technical field [0001] The invention relates to the field of animal genetics and breeding, in particular to a SNP molecular marker affecting the fineness traits of cashmere cashmere and its application. Background technique [0002] Cashmere goat is a kind of goat breed with both meat and meat formed after long-term natural selection and artificial breeding. It has the advantages of drought resistance and severe cold resistance. It is mainly distributed in 25°-55° north latitude and 40°-125° east longitude. Famous cashmere goat breeds mainly include Inner Mongolia cashmere goats, Liaoning cashmere goats, northern Shaanxi white cashmere goats, Qaidam cashmere goats, Xinjiang goats, Tibet goats, etc. The cashmere goat breeds are as many as three-fifths of the world's cashmere goat breeds. It is a livestock breed with independent breeding capacity in our country. [0003] Cashmere is unmyelinated hair, which grows from secondary hair follicles. The diameter of the hair follicl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156Y02P60/87
Inventor 苏蕊王凤红张磊吕琦王瑞军王志英张燕军李金泉
Owner INNER MONGOLIA AGRICULTURAL UNIVERSITY
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