SNP molecular marker influencing cashmere fine character of cashmere goat and application of SNP molecular marker
A molecular marker, cashmere goat technology, applied in the field of animal genetics and breeding, can solve the problems that are not conducive to the excellent cashmere production traits of cashmere goats, reduce the economic benefits of breeding cashmere goats, slow down the breeding and improvement of cashmere goats, etc., so as to improve international competitiveness and improve The effect of breeding efficiency and saving breeding cost
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[0030] 1 Experimental materials
[0031] 1.1 Equipment and reagents
[0032] Reagents: petroleum ether, 75% alcohol, 10% SDS, proteinase K, STE, saturated Tris phenol, DNA extraction solution 25:24:1 phenol: chloroform: isoamyl alcohol, ice isopropanol, enzyme-free water, agarose, TAE, nucleic acid dye, DL500 DNA Maker, etc.
[0033] Equipment: Auricular forceps, surgical scissors, pipette gun, parafilm, OFDA fiber fineness tester, constant temperature culture shaker, centrifuge, NanoDrop 2000C ultra-micro spectrophotometer, ABI Veriti gradient PCR instrument, electronic balance, DYY -6C bistable electrophoresis instrument power supply, electrophoresis tank, Bio-Rad ChemiDoc XRS+imager, etc.
[0034] 1.2 Sample collection
[0035] Select 1,920 Inner Mongolia cashmere goats from Inner Mongolia Yiwei Cashmere Goat Co., Ltd., use surgical scissors to collect villi samples behind the scapula on the abdominal midline of individual cashmere goats, put them into plastic bags, and ...
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