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Method for converting ginsenoside in ginseng adventitious roots

A technology of ginsenosides and ginseng, which is applied in the field of plant tissue culture, can solve the problems of cumbersome saponin conversion methods, and achieve the effects of shortening the induction time, simple steps, and large quantities

Active Publication Date: 2021-11-16
SHANDONG ANRAN NANOMETRE IND DEV CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In addition, the current conversion method of saponins in adventitious roots is relatively cumbersome. If a conversion method with high conversion rate of rare saponins can be obtained, which is convenient and quick, the efficiency can be further improved

Method used

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  • Method for converting ginsenoside in ginseng adventitious roots
  • Method for converting ginsenoside in ginseng adventitious roots
  • Method for converting ginsenoside in ginseng adventitious roots

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0081] (1) Induction of adventitious roots

[0082] After 20-year-old wild ginseng, remove the reed head, stalk, and main root after cleaning, disinfection and sterilization, cut into thin slices with a width of 0.6cm, a length of 0.7cm and a thickness of 0.3mm, and inoculate them into induction medium, at 22±1°C Adventitious roots of wild ginseng were induced by dark culture for 4 to 5 weeks under conditions; wherein, the induction medium included 4mg / L naphthaleneacetic acid, 0.6mg / L gibberellin, 0.4mg / L kinetin, 0.1g / L citric acid, 0.05g / L L ascorbic acid, 30g / L sucrose, 3g / L Phytogel, 1.55g / L B5 medium and 1.21g / L WPM medium, pH value is 5.8.

[0083] (2) Subculture of adventitious roots

[0084] Inoculate the adventitious roots of wild ginseng obtained in step (1) into the same induction medium as in step (1), and culture in dark under the same conditions for 4 to 5 weeks;

[0085] (3) The cultivation of adventitious roots

[0086] Cut the adventitious roots of wild gi...

Embodiment 2

[0088] (1) Induction of adventitious roots

[0089] After cleaning, disinfecting and degerming the reed heads of 6-year-old garden ginseng, cut them into thin slices with a width of 0.5 cm, a length of 0.6 cm and a thickness of 0.3 mm, inoculate them into induction medium, and culture them in dark at 22±1°C for 4-5 days. Zhou induced adventitious roots; wherein, the induction medium included 6mg / L naphthaleneacetic acid, 0.2mg / L gibberellin, 0.4mg / L kinetin, 1.2g / L citric acid, 0.1g / L ascorbic acid, 20g / L sucrose, 5g / L plant gel, 4g / L B5 medium and 1.8g / L WPM medium, pH value is 5.6.

[0090] (2) Subculture of adventitious roots

[0091] Inoculate the adventitious roots obtained in step (1) into the same induction medium as in step (1), and culture in dark under the same conditions for 4 to 5 weeks;

[0092] (3) The cultivation of adventitious roots

[0093] Cut the adventitious roots obtained in step (2) into tissues with a length of about 1 cm, inoculate them into liquid ...

Embodiment 3

[0096] (1) Induction of adventitious roots

[0097] After cleaning, disinfecting and sterilizing the horns of 10-year-old understory ginseng, cut them into thin slices with a width of 0.7 cm, a length of 0.7 cm, and a thickness of 0.5 mm, inoculate them into induction medium, and culture them in dark at 22±1°C for 4~ Adventitious roots were induced in 5 weeks; wherein, the induction medium included 5mg / L naphthaleneacetic acid, 1mg / L gibberellin, 0.1mg / L kinetin, 0.75g / L citric acid, 0.03g / L ascorbic acid, 40g / L sucrose, 4g / L Phytogel, 2g / L B5 medium and 1g / L WPM medium, pH 6.0.

[0098] (2) Subculture of adventitious roots

[0099] Inoculate the adventitious roots obtained in step (1) into the same induction medium as in step (1), and culture in dark under the same conditions for 4 to 5 weeks;

[0100] (3) The cultivation of adventitious roots

[0101] Cut the adventitious roots obtained in step (2) into tissues with a length of about 2 cm, inoculate them into liquid mediu...

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Abstract

The invention discloses a method for converting ginsenoside in ginseng adventitious roots. The method comprises the following steps of (1) preparing a ginseng adventitious root sample; (2) adding alkali liquor into the ginseng adventitious root sample, heating to 85-125 DEG C, preserving heat for 20-60 minutes, cooling to room temperature, then repeating the heating, heat preservation and cooling processes; and (3) treating the sample, and detecting the content of the ginsenoside. In addition, a culture method of the ginseng adventitious roots comprises the following steps of cleaning, disinfecting and slicing mature ginseng, and inoculating into an induction culture medium to induce the ginseng adventitious roots; inoculating the obtained ginseng adventitious roots into the induction culture medium again for subculture and propagation; cutting the obtained ginseng adventitious roots into segments, inoculating the segments into a liquid culture medium, and culturing to obtain the adventitious roots. According to the method, the adventitious roots are generated through one-step induction of all parts of the mature ginseng, the induction step can be simplified, and the induction time can be shortened; the method for converting the ginsenoside in the adventitious roots is simple in steps and easy to operate, and the conversion rate of rare saponin is high.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a method for transforming ginsenosides in adventitious roots of ginseng. Background technique [0002] Ginseng (Panax ginseng C.A.Mey.) is a plant of the genus Panax of Araliaceae, distributed in China, Japan, and Korea. Its rhizome is a valuable Chinese medicinal material, known as the "King of Herbs". Ginseng is sweet, slightly bitter, and slightly warm. It has the functions of invigorating vital energy, restoring pulse and strengthening the spleen, nourishing the spleen and lung, promoting body fluid and nourishing blood, calming the nerves and improving intelligence, etc. Wound thirst, palpitations, insomnia, etc. Ginsenoside is the main active ingredient of ginseng, which has the effects of anti-fatigue, anti-aging, regulating the central nervous system, improving immunity, improving cardiovascular and cerebrovascular insufficiency, and inhibiting t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 高秀君刘冰张明臣闫培生
Owner SHANDONG ANRAN NANOMETRE IND DEV CO LTD