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Preparation method of polyene phosphatidylcholine

A technology of polyene phosphatidylcholine and soybean phospholipids, which is applied in the direction of edible phospholipid compositions, chemical instruments and methods, compounds of Group 5/15 elements of the periodic table, etc., can solve problems such as high cost and complicated process, and achieve The effect of improving quality and simple method

Pending Publication Date: 2021-11-23
海思科制药(眉山)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] The preparation method process of the above-disclosed phosphatidylcholine is complicated, and some will use such expensive chromatography filler as SephadexLH-20, and the cost is high; and the soybean phosphatidylcholine (PC -90), its active ingredient DLPC content is at 40%-50%, and its DLPC content of commercially available Aventis company polyene phosphatidylcholine is about 52%

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The preparation of embodiment 1 silver nitrate chromatography silica gel

[0028] Weigh 50Kg of ordinary silica gel (100-200 mesh, coarse pores), add 100L of an aqueous solution containing 5Kg of silver nitrate (so that it is completely submerged in silica gel), and heat in a boiling water bath for 20-30min (intermittent stirring). After cooling to room temperature, filter with suction, and then activate in a vacuum oven at 120°C for 20 hours to obtain silver nitrate chromatography silica gel.

Embodiment 2

[0029] The preparation of embodiment 2 polyene phosphatidylcholine

[0030]Take 40g of soybean lecithin, dissolve it with methanol, then mix the sample with 40g of silica gel (100 mesh), and take another 800g of silver nitrate chromatographic silica gel dry method and install it in a dark chromatographic column (the lower end is pre-filled with 80g of ethylenediaminetetraacetic acid). Pack on the chromatographic column, first use the chloroform that volume ratio is 95:5: the mixed solvent system of methanol carries out eluting impurity removal; Use the chloroform that volume ratio is 80:20 again: methanol mixed solvent system elutes, collects eluent, After vacuum concentration and drying, 27.4 g of polyene phosphatidylcholine was obtained. Through HPLC analysis, the purity of polyene phosphatidylcholine was 98.3%, and the active ingredient 1,2-dilinoleoylphosphatidylcholine (DLPC) content was 69.8%.

Embodiment 3

[0031] The preparation of embodiment 3 polyene phosphatidylcholine

[0032] Take 40g of soybean lecithin, dissolve it with methanol, then mix the sample with 40g of silica gel (100 mesh), and take another 800g of silver nitrate chromatography silica gel dry method and install it in a dark chromatographic column (the lower end is pre-filled with 100g of bis(1,6-hexylene) Triamine pentamethylene phosphonic acid), the sample is loaded on the chromatographic column, first with the chloroform that volume ratio is 95:5: the mixed solvent system of methanol carries out eluting impurity removal; Then with the chloroform that volume ratio is 70:30: Eluted with a methanol mixed solvent system, collected the eluent, concentrated in vacuo, and dried to obtain 28.2 g of polyene phosphatidylcholine. According to HPLC analysis, the purity of polyene phosphatidylcholine was 96.1%, and the active ingredient DLPC content was 65.7%. .

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Abstract

The invention provides a preparation method of polyene phosphatidylcholine. Soybean phospholipid is used as a raw material, and the polyene phosphatidylcholine is prepared by silver nitrate chromatography silica gel column chromatography separation. The content of the main active ingredient 1,2-dilinoleyl phosphatidylcholine (DLPC) of the polyene phosphatidylcholine product prepared by the method disclosed by the invention is 60% or above, and the quality of the polyene phosphatidylcholine product is greatly improved.

Description

technical field [0001] The invention relates to a preparation method of polyene phosphatidylcholine, which belongs to the technical field of pharmaceutical engineering. Background technique [0002] Polyene Phosphatidylcholine (PPC) is a phosphatidylcholine extracted from soybean lecithin, which is a phosphatidylcholine containing linoleic acid, linolenic acid and oleic acid on the triglyceride skeleton of lecithin. Base, whose main active ingredient is 1,2-dilinoleoylphosphatidylcholine (1,2-Dilinoleoyl-sn-Glycero-3-Phosphatidylcholine, DLPC), also known as 1,2-dilinoleoyl phosphatidylcholine . Polyene phosphatidylcholine is chemically consistent with important endogenous phospholipids, and has the following physiological functions: restore damaged liver function and enzyme activity, regulate liver energy balance, promote liver tissue regeneration, Converts sexual fats and cholesterol into easily metabolizable forms and stabilizes bile. DLPC has very important biological...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07F9/10G01N30/02
CPCC07F9/103G01N30/02G01N2030/027
Inventor 李麒麟赵德余杨平
Owner 海思科制药(眉山)有限公司
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