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Nano preparation loaded with gene diagnosis probe and/or anti-pulmonary fibrosis drug and preparation method of nano preparation

A nano-preparation, pulmonary fibrosis technology, applied in the fields of nanotechnology, nanotechnology, nanotechnology for materials and surface science, can solve the problem of difficult diagnosis of the disease course, achieve inhibition of excessive activation and block early pulmonary fibrosis , improve the accuracy and standardization of treatment

Pending Publication Date: 2021-11-26
JINZHOU MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Objective: In order to overcome the clinical problem that the course of disease is difficult to diagnose in the treatment of pulmonary fibrosis, the present invention discloses a nano-preparation loaded with gene diagnostic probes and / or anti-pulmonary fibrosis drugs and its preparation method

Method used

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  • Nano preparation loaded with gene diagnosis probe and/or anti-pulmonary fibrosis drug and preparation method of nano preparation
  • Nano preparation loaded with gene diagnosis probe and/or anti-pulmonary fibrosis drug and preparation method of nano preparation
  • Nano preparation loaded with gene diagnosis probe and/or anti-pulmonary fibrosis drug and preparation method of nano preparation

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Experimental program
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Embodiment 1

[0064] Synthesis and preparation of embodiment 1 nano preparation composition, such as figure 1 The schematic diagram of the preparation process of GCL@QY nano-preparation is shown: 1. Preparation of nanoparticles containing sensitive lipid fragment L, DSPE-mPEG, soybean lecithin (PC) and cholesterol

[0065] 1. Preparation of diagnostic probes containing sensitive lipid fragment L-loaded genes and anti-pulmonary fibrosis drugs (GCL@QY)

[0066] First, the hydrophobic / hydrophilic anti-pulmonary fibrosis drug Q is dissolved in an organic solvent or aqueous solution, and X-mPEG, phospholipid P, cholesterol C and sensitive lipid fragment L are dissolved in an organic solvent and mixed thoroughly. Nano-preparations loaded with anti-pulmonary fibrosis drugs were prepared by thin-film dispersion method and reverse evaporation method. Among them, the gene diagnostic probe Y realizes the precise diagnosis of early pulmonary fibrosis in the cytoplasm of injured AECs II; on the other h...

Embodiment 2

[0089] Example 2 The loading situation of nano-preparation to medicine

[0090] The nano-preparation of GCL@QY was prepared according to the method described in Example 1. After adding 500 μL of ethanol solution to the nano-preparation to break the emulsion, the carrier material and drug of the nano-preparation were scanned by a full-wavelength UV spectrophotometer, and the wavelength range was 200 nm. -800nm. At the same time, the gene diagnostic probe loaded on the carrier material was screened, and the needle was tested by agarose gel electrophoresis. Finally, the prescription ratio of 1:5 was selected as the optimal prescription for the preparation of nano-preparation, as shown in figure 2 shown.

[0091] In this implementation, the loading of pirfenidone is as follows: image 3 As shown, after the nano-preparation is prepared by the film dispersion method, ethanol is added to break the emulsion, and then the spectral absorption of the nano-preparation between 200nm and...

Embodiment 3

[0092] The particle size distribution figure of embodiment 3 best nano-preparation GCL@QY

[0093] After preparing the nano-preparation of GCL@QY according to the method described in Example 1, the particle size distribution of GCL@QY was characterized by using a Malvern particle size analyzer. The solution volume was 2mL, the number of detection cycles was 13, and 3 parallel detection Second-rate.

[0094] The release curve of the nano-preparation measured in this embodiment is as follows: Figure 4 As shown, the particle size distribution of GCL@QY is between 10nm-1000 nm, and the average particle size of the nano-preparation is 100.4nm. The nano-preparation has uniform particle size distribution and uniform shape, which is suitable for subsequent cell experiments and animal experiments, and also provides operability for future clinical transformation.

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Abstract

The invention discloses a nano preparation loaded with a gene diagnosis probe and / or an anti-pulmonary fibrosis drug and a preparation method of the nano preparation. The nano preparation is simultaneously loaded with the gene diagnosis probe and the anti-pulmonary fibrosis drug, the gene diagnosis probe realizes diagnosis of early pulmonary fibrosis by virtue of a fluorescence energy resonance transfer principle, and cooperates with the anti-pulmonary fibrosis drug to regulate steady-state balance of type II alveolar epithelial cells, so that the aim of treating the early pulmonary fibrosis through diagnosis and treatment in a cooperative manner is achieved. On the other hand, the nano preparation containing Reactive Oxygen Species (ROS) specific sensitive lipid fragments and a carrier thereof are utilized, and instantaneous release of the anti-pulmonary fibrosis drug is promoted by virtue of the ROS specific sensitive lipid fragments, so that the aim of regulating the early pulmonary fibrosis through diagnosis and treatment in a cooperative manner is achieved, and a new way and strategy are provided for treatment of reversing the early pulmonary fibrosis.

Description

technical field [0001] The invention discloses a nano-preparation loaded with a gene diagnosis probe and / or an anti-pulmonary fibrosis drug and a preparation method thereof. Background technique [0002] Pulmonary fibrosis is a chronic pulmonary interstitial disease characterized by progressive decline in lung function, characterized by high clinical morbidity and mortality. Studies have confirmed that the abnormal injury of type II alveolar epithelial cells (Alveolar epithelial cells, AEC II) is the key cause of the occurrence and progression of pulmonary fibrosis. Damaged AEC II intracellular mitochondria secrete a large amount of reactive oxygen species (Reactive oxygen species, ROS) into the cytoplasm due to oxidative stress imbalance, which stimulates the pro-inflammatory factor interleukin 1β by activating a variety of intracellular cysteine ​​proteases in AECs II (Interleukin 1β, IL 1β) and interleukin 13 (Interleukin 13, IL 13) are overexpressed in the cytoplasm, re...

Claims

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Application Information

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IPC IPC(8): A61K47/69A61K47/60A61K31/4418A61K49/00A61P11/00B82Y5/00B82Y20/00B82Y30/00B82Y40/00
CPCA61K31/4418A61K47/60A61K47/6911A61P11/00A61K49/0084A61K49/0054B82Y5/00B82Y20/00B82Y30/00B82Y40/00
Inventor 常鑫
Owner JINZHOU MEDICAL UNIV
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