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Antagonist ed5 and its application

A technology of antagonistic bacteria and bacterial agents, applied in the field of microorganisms, can solve problems such as mutations, food safety issues, and drug resistance of pathogenic bacteria

Active Publication Date: 2022-02-08
渤海水产科技(滨州)有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the widespread use of antibiotics can cure many related diseases, it also brings a series of unavoidable serious problems. The drugs in the product enter the human body along with the aquatic products, causing serious food safety problems, etc. These problems have added many direct or indirect adverse effects to human health and life.

Method used

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  • Antagonist ed5 and its application
  • Antagonist ed5 and its application
  • Antagonist ed5 and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Example 1 Isolation and selection of intestinal bacteria and pathogenic bacteria

[0047] (1) Isolation of intestinal bacteria

[0048] Select fresh Japanese hairtail purchased from Weihai Yuanyao Market ( Trichiurus lepturus). Clean the fish with sterilized seawater, wipe the body surface of the fish with 75% alcohol, cut the abdomen of the fish with scissors, take the complete intestinal tract, and place it in PBS buffer solution for later use; squeeze out the contents of the intestinal tract before sterilization Centrifuge tubes, dilute the homogenate with sterile seawater at a ratio of 1:9.

[0049] Direct coating culture method: Take 10ml of the homogenate and place it in a 50ml Erlenmeyer flask filled with 40ml of sterilized seawater (the bottom of the bottle is covered with glass beads before sterilization), put it in a shaker at 20°C, 120rpm, and culture on a shaker 30min to obtain the bacterial suspension mother liquor. In the ultra-clean workbench, use st...

Embodiment 2

[0054] Example 2 Screening of antagonistic bacteria

[0055] The bacteria to be tested were planted on a plate coated with pathogenic bacteria, and placed in a 28°C incubator for 24 hours. Both the bacteria to be tested and the pathogenic bacteria grew well, and the inhibition zone was observed. The experimental results are shown in Table 1. 2.

[0056] Among the 6 pathogenic bacteria, only Staphylococcus aureus was Gram-positive bacteria, and the remaining 5 pathogenic bacteria were all Gram-negative bacteria. When the intestinal bacteria to be tested have an antagonistic effect on pathogenic bacteria, a transparent and well-defined inhibition zone will appear around the colonies of the bacteria to be tested, such as figure 1 ; When the intestinal bacteria to be tested do not have antagonism to pathogenic bacteria, no zone of inhibition will appear, and the antagonism is negative, such as figure 2 .

[0057] Table 1 Primary screening results of antagonistic bacteria

[...

Embodiment 3

[0063] Example 3 In Vitro Hemolysis Experiment

[0064] The screened antagonistic bacteria to be tested were inoculated on a blood agar plate and cultured upside down in a 28°C incubator. It can be seen from Table 3 that the antagonistic bacteria ED5 did not produce hemolytic circles.

[0065] Table 3 In vitro hemolysis test results

[0066]

[0067] When the bacteria have hemolytic ability and enter the fish body, they will cause a large number of red blood cells to rupture in the body, causing hemoglobin to leak out of the cells, thereby triggering a series of reactions such as anemia and destruction of the immune system. Table 3 shows that the antagonist ED5 does not produce hemolysis, indicating that the antagonist has certain safety.

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Abstract

The present invention provides an antagonistic bacteria ED5 and its application, and relates to the technical field of microbes. The present invention takes common marine fish intestinal flora as the research object, and screens out a kind that can effectively antagonize six common fish pathogenic bacteria Antagonist ED5. As a probiotic, the antagonistic ED5 not only has obvious antibacterial effect on various pathogenic bacteria, but also has a certain affinity with other probiotics, and is expected to exert a synergistic antibacterial effect.

Description

technical field [0001] The invention relates to the technical field of microbes, in particular to an antagonistic bacteria ED5 and its application. Background technique [0002] In recent years, the misuse of antibiotics has become an important topic of concern. Although the widespread use of antibiotics can cure many related diseases, it also brings a series of unavoidable serious problems. The drugs in the product enter the human body along with the aquatic products, causing serious food safety problems, etc. These problems have added many direct or indirect adverse effects to human health and life. Therefore, the development of alternatives to various antibiotics has become a research hotspot. [0003] With the continuous deepening of people's research on intestinal flora, the intestinal microorganisms of fish have gradually entered the field of vision of researchers and have attracted widespread attention. Many different kinds of microorganisms live in the gastrointes...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A61K35/742A61P31/04C12R1/07
CPCC12N1/20A61K35/742A61P31/04
Inventor 陈锚赵丽华叶慧马士玉时公民张恒壮于胜涛
Owner 渤海水产科技(滨州)有限公司
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