A structure and function bionic urethral stent and preparation method thereof

Abstract

The invention provides a bionic urethral scaffold with structure and function and a preparation method thereof; the preparation method is as follows: (1) mix the oxidized bacterial cellulose nanofiber dispersion and the acellular matrix solution and pour it into the mold I until it is completely filled and frozen Dry to obtain an uncrosslinked porous scaffold; (2) put the uncrosslinked porous scaffold into a crosslinking agent solution, rinse with water after crosslinking, and then freeze-dry to obtain a crosslinked porous scaffold; (3) oxidize the bacterial fiber The mixed liquid II composed of plain nanofibers, modified or unmodified natural polymer materials, gel-forming aids and water is poured into the silica gel mold II until it is completely filled, and the cross-linked porous scaffold is fixed on the mold II, so that Contact with the mixed solution II and let it stand for a period of time to obtain a structural and functional bionic urethral scaffold; the prepared scaffold is composed of a hydrogel layer of the bionic urethral mucosa and a porous layer of the bionic urethral spongy body, and can be rapidly epithelialized and Vascularized, has a good repair effect on urethral defects.

Description

technical field [0001] The invention belongs to the technical field of urethral stents, and relates to a urethral repair stent and a preparation method thereof, in particular to a structural and functional bionic urethral stent and a preparation method thereof. Background technique [0002] Urethral stricture is a urological disease caused by trauma, infection, iatrogenic operation, etc. The global incidence of urethral stricture is 0.3%, especially complex long-segment urethral stricture, which seriously affects the quality of life of patients. Its repair and reconstruction have always been A major problem facing urology clinical practice. Autologous tissue transplantation, such as oral mucosa, penile flap, bladder mucosa, is currently the main measure for the clinical treatment of urethral stricture. However, this method has the limitations of "at the expense of normal tissue" and "limited tissue sources for transplantation". In recent years, the development of tissue en...

AI Technical Summary

Problems solved by technology

The Chinese invention patent with the publication number CN110960727A "a tissue-engineered urethral stent graft and its preparation method and application" discloses a degradable nanofibrous tube of polymer material inoculated with epithelial cells and smooth muscle cells as a tissue-engineered urethra For grafts, epithelial cells are used as seed cells to promote the rapid formation of epithelial layers after graft implantation. However, the source of epithelial cells is limited, the culture process is cumbersome, and sequelae will appear at the site of sampling.