Streptomyces fulvissimus for producing anthracimycin and production method of streptomyces fulvissimus
A technology of Streptomyces flavus and anthrax mycin, which is applied in the direction of microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the problems of low yield and restrictions on the development and utilization of anthrax toxin, and achieve low cost and easy mass production , Environmentally friendly effect
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[0030] In the present invention, the preparation method of the seed solution preferably includes: inoculating the Streptomyces fulgus YINM00001 on the activation medium, and performing activation culture to obtain an activated strain; the activation medium uses water as a solvent, Comprising the following components in mass and volume percentages: 0.2-0.8% yeast powder, 0.2-0.8% glucose, 0.1-0.6% malt extract, 0.1% normal saline, 1.5% agar and the rest of water, the activation medium It also includes 0.5~3mg / L multivitamin, pH value 6.0~8.0;
[0031] The activated strain is inoculated in the seed medium for seed culture to obtain a seed solution; the seed medium includes the following components in mass and volume percentages: 0.2-0.8% yeast powder, 0.2-0.8% glucose, 0.1-0.6% Malt extract, 0.1% physiological saline and the rest of water, the activation medium also includes 0.5-3 mg / L multivitamins, and the pH value is 6.0-8.0.
[0032] The activation culture in the present in...
Embodiment 1
[0039] The endophytic actinomycete Streptomyces fulgus YINM00001 obtained from Cicada chinensis in Kunming, Yunnan. The following paragraph describes the separation process.
[0040] Sterilize the surface of the chrysalis collected from Kunming, Yunnan through sodium hypochlorite solution (available chlorine concentration 5.0%), 75% ethanol soaking and sterile water washing process, and then place the plant tissues that have been processed into small pieces at 121°C to sterilize. Bacteria activation medium (mass volume percentage: 0.4% yeast powder, 0.4% glucose, 0.3% malt extract, 0.1% saline, 1mg / L multivitamin, 1.5% agar, pH 7.0) surface, cultured at 28°C for 7 days , using the activation medium sterilized at 121°C to continuously purify for 5 generations to obtain multiple strains of endophytic bacteria, which were determined to be Streptomyces fulvissimus, strain Name it YINM00001.
Embodiment 2
[0042] Cell activation: Inoculate Streptomyces fulgus YINM00001 on the slope of the activation medium that has been sterilized at 121°C for 30 minutes, and culture it in a constant temperature incubator at 28°C for 3 days.
[0043] Strain preparation: insert the activated thalline into the seed medium sterilized at 121°C for 30 minutes (mass volume percentage: 0.4% yeast powder, 0.4% glucose, 0.3% malt extract, 0.1% normal saline, 1mg / L compound vitamins, pH 7.0), 28° C., 200 rpm for 3 days of fermentation.
[0044] Fermentation process: Add the prepared strains according to the volume fraction of 5% into the fermentation medium (4% soluble starch, 1% corn extract, 0.05% KH 2 PO 4 , 0.025% MgSO 4 , 0.004% ZnSO 4 ·7H 2 O, 0.01% Methionine, 0.01% Vitamin B12, 0.5% CaCO 3 , pH 7.0), 28° C., 200 rpm for 10 days of fermentation.
[0045] After the fermentation, the fermented liquid was analyzed by high performance liquid chromatography, and the concentration of anthracisin in...
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