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Extraction method of caulerpa lentillifera polysaccharide

The technology for polysaccharides from Pteridophyta and Pteridophyta long-stemmed is applied in the field of polysaccharide extraction of P. long-stemmed, and can solve the problems such as hindering the application and popularization of polysaccharides of P. long-stemmed, complicated preparation process and low polysaccharide yield, Achieve high yield, simple process, and improve the effect of extraction rate

Pending Publication Date: 2021-12-21
GUANGXI ACADEMY OF FISHERY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, when separating and extracting the polysaccharides of Vitis vinifera, the extraction methods of water extraction, alcohol precipitation and alkaline extraction have problems such as low polysaccharide yield, high cost and complicated preparation process, so that the production efficiency is low and the product Poor quality hinders the application and popularization of polysaccharides from Grape fern

Method used

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  • Extraction method of caulerpa lentillifera polysaccharide
  • Extraction method of caulerpa lentillifera polysaccharide
  • Extraction method of caulerpa lentillifera polysaccharide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A kind of extraction method of long-stem grape fern algae polysaccharide, it comprises the following steps:

[0027] (1) Wash Botrytis long-stem algae with seawater, and then wash three times with distilled water, then place in an oven and dry at 50°C, and then crush and pass through a 50-mesh sieve to obtain Botrytis long-stemmed algae powder. The algae powder is dried and stored for later use;

[0028] (2) Take Botrytis elongatum powder and add ultrapure water to mix it uniformly to obtain mixed liquid A. The ratio of the mass of Botrytis elongatus powder to the volume of ultrapure water is 1g:45ml, and then add Add hydrochloric acid with a mass concentration of 10% or a sodium hydroxide solution with a mass concentration of 5% to adjust the pH to 6, add papain, and extract at 60°C for 3 hours, and stir the mixed solution A every 30 minutes. Stir 2min each time, obtain mixed solution B, the add-on of described papain is 2% of mixed solution A quality;

[0029] (3) I...

Embodiment 2

[0032] A kind of extraction method of long-stem grape fern algae polysaccharide, it comprises the following steps:

[0033] (1) Wash Botrytis long-stem algae with seawater, and then wash three times with distilled water, then place in an oven and dry at 50°C, and then crush and pass through a 50-mesh sieve to obtain Botrytis long-stemmed algae powder. The algae powder is dried and stored for later use;

[0034] (2) Take Botrytis longstemma powder and add ultrapure water to mix it uniformly to obtain mixed solution A. The ratio of the mass of Botrytis longstemma powder to the volume of ultrapure water is 1g:50ml, and then add Add hydrochloric acid with a mass concentration of 10% or a sodium hydroxide solution with a mass concentration of 5% to adjust the pH to 6, add papain, and extract at 60°C for 4 hours, and stir the mixed solution A every 30 minutes. Stir 2min at every turn, obtain mixed solution B, the add-on of described papain is 2.5% of mixed solution A quality;

[0...

Embodiment 3

[0038] A kind of extraction method of long-stem grape fern algae polysaccharide, it comprises the following steps:

[0039] (1) Wash Botrytis long-stem algae with seawater, and then wash three times with distilled water, then place in an oven and dry at 50°C, and then crush and pass through a 50-mesh sieve to obtain Botrytis long-stemmed algae powder. The algae powder is dried and stored for later use;

[0040] (2) Take Botrytis elongatum powder and add ultrapure water to mix it uniformly to obtain mixed liquid A. The ratio of the mass of Botrytis elongatus powder to the volume of ultrapure water is 1g:40ml, and then add Add hydrochloric acid with a mass concentration of 10% or a sodium hydroxide solution with a mass concentration of 5% to adjust the pH to 6, add papain, and extract at 50°C for 3 hours, and stir the mixed solution A every 30 minutes. Stir 2min each time, obtain mixed solution B, the add-on of described papain is 2% of mixed solution A quality;

[0041] (3) Put...

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Abstract

The invention discloses an extraction method of caulerpa lentillifera polysaccharide. The method comprises the following steps: cleaning caulerpa lentillifera with seawater and distilled water in sequence, and then drying and crushing to obtain caulerpa lentillifera powder; adding ultrapure water and papain into the caulerpa lentillifera powder, extracting for 2-4 hours, deactivating enzyme under the condition of boiling water bath, and performing centrifugal separation to obtain supernate and algae residues; carrying out negative pressure concentration on the supernate, adding ethanol for alcohol precipitation, and centrifuging to remove the ethanol on the upper layer to obtain a precipitate A; and extracting the caulerpa lentillifera residues according to the same steps to obtain a precipitate B, combining the precipitate A and the precipitate B, completely dissolving in ultrapure water, and freeze-drying to obtain the caulerpa lentillifera polysaccharide. The method can improve the extraction rate of caulerpa lentillifera polysaccharide, and has the advantages of easiness in preparation, low cost, no pollution, high yield and the like.

Description

technical field [0001] The invention belongs to the technical field of extracting the polysaccharides from the long-stem grape fern, and in particular relates to a method for extracting the polysaccharide from the long-stem grape fern. Background technique [0002] C. lentillifera is a genus of green algae, commonly known as sea grapes (scientific name: Caulerpa lentillifera) native to Ryukyu. Its upright branches resemble grapes in shape, and its algae can be divided into three parts: stolons, upright branches and filamentous rhizomes. , globose branchlets covering the entire main axis of erect branches. The whole algal body is a multinucleated cell, although there are many nuclei, but there is no cell wall between each other. Grape fern algae is a nutritious edible green algae, containing a variety of amino acids, polysaccharides and vitamins needed by the human body, omega-6 fatty acids such as oleic acid, arachidonic acid, etc., which are essential in grape fern algae ...

Claims

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Application Information

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IPC IPC(8): C08B37/00
CPCC08B37/0003
Inventor 赵永贞潘传燕童艳梅陈晓汉杨春玲刘青云李强勇罗帮曾地刚陈秀荔彭敏冯鹏霏蒙源陈田聪李旻王卉
Owner GUANGXI ACADEMY OF FISHERY SCI