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In-vitro rapid propagation method for seed summer squash

A technology of zucchini and in vitro is applied in the field of rapid in vitro propagation of zucchini for seeds, which can solve the problems of farmers and seed enterprises, such as loss and long cycle, and achieve the effects of promoting division and differentiation, rooting, easy seedling formation, and alleviating impact.

Active Publication Date: 2021-12-24
INNER MONGOLIA AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing zucchini seeds need about 70 days from cotyledon inoculation to seedling regeneration, which is a long period.
[0004]At the same time, in recent years, the damage of zucchini for seeds such as viral diseases, powdery mildew, and wilt has become more serious, especially viral diseases. Since 2016, Inner Mongolia, Xinjiang, Heilongjiang Commonly occurs in other places, and the production reduction reaches 10% to 50%, causing huge losses to farmers and seed companies

Method used

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  • In-vitro rapid propagation method for seed summer squash
  • In-vitro rapid propagation method for seed summer squash
  • In-vitro rapid propagation method for seed summer squash

Examples

Experimental program
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Effect test

Embodiment 1

[0035] A kind of in vitro rapid propagation method of zucchini for seeds, comprising the following steps:

[0036] S1. Select zucchini seeds of the variety Hongchang 211, soak them in warm water at 50°C for 30 minutes, put the seeds in 75% ethanol solution for 15 seconds for surface disinfection after shelling, rinse with sterile water for 3 times, and then use 4% mass fraction Disinfect with NaClO solution for 15 minutes, rinse with sterile water for 6 times, and complete the disinfection treatment;

[0037] S2. Inoculate the sterilized seeds into MS medium, inoculate 5-7 seeds in each culture bottle, and cultivate them in a dark environment at 25±1° C. to germinate;

[0038] S3. Primary culture: After germination and culture, carefully remove the hypocotyls of zucchini seedlings for seeds in the ultra-clean workbench, cross-cut the cotyledons with a scalpel, carefully remove the part of the far hypocotyl, and take 1 of the near hypocotyl. / 2 cotyledon, divide it into two, a...

Embodiment 2

[0044] A method for in vitro rapid propagation of zucchini for seeds, the difference from Example 1 is that the variety of zucchini seeds for seeds selected is Jinfeng No. 8.

Embodiment 3

[0046] A method for in vitro rapid propagation of zucchini for seeds, the difference from Example 1 is that the addition amount of 6-BA in the medium in S3 is 0.5 mg / L, and the addition amount of TDZ is 1.5 mg / L.

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PUM

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Abstract

The invention belongs to the technical field of plant in-vitro culture, and particularly discloses an in-vitro rapid propagation method for seed summer squash. The method comprises the following steps of S1, carrying out disinfection treatment on shelled seed summer squash seeds; S2, inoculating the disinfected seeds into an MS culture medium to germinate the seeds; S3, primary culture; S4, rooting culture; S5, domesticating and transplanting. According to the in-vitro rapid propagation method for the seed summer squash, the seed summer squash tissue culture seedlings with low pollution rate can be obtained, and the influence of seed-borne diseases is overcome; the method has the advantages of short seedling induction time, developed root systems of rooted plants, and shortening of the seedling raising period of the seed summer squash.

Description

technical field [0001] The invention belongs to the technical field of plant in vitro cultivation, and specifically discloses a method for rapid in vitro propagation of zucchini for seeds. Background technique [0002] Zucchini for seeds is an annual herbaceous plant belonging to Cucurbitaceae and pumpkin. It is native to southern North America. It was introduced and cultivated from Europe in China in the middle of the 19th century, and it is distributed all over the world. Its grains are rich in nutrients such as fat, protein, minerals and vitamins. The content of crude fat in zucchini seeds for seeds is 40.4% to 53.02%, the content of unsaturated fatty acids is 56.44% to 61.34%, and the content of linoleic acid is 10.37% to 51.19%. %, the protein content reaches 34% to 40%, and has high nutritional value. In addition, the seeds are rich in trigonelline, potassium and phosphorus, which have the effects of preventing cancer, lowering fat, lowering blood sugar, relieving itc...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/002
Inventor 王萍李石恒
Owner INNER MONGOLIA AGRICULTURAL UNIVERSITY