Application of folium artemisiae argyi polysaccharide in regulating human intestinal flora structure
A technology for polysaccharide and intestinal flora of mugwort leaves, which is applied in the fields of application, food ingredients containing polysaccharide/gum, medical preparations containing active ingredients, etc.
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Embodiment 1
[0027] Example 1: Preparation of Artemisia argyi Polysaccharides
[0028] Take 200g of dried mugwort leaves, add 700ml of 95% ethanol, reflux in a water bath for 2 hours, degrease twice, filter through a filter cloth, collect the filter residue, dry the filter residue as much as possible, dry the filter residue at 50°C, or air dry naturally. The mass volume ratio of degreased moxa residue to water is 1:20 (1g / 20ml), heated at 99°C for 2.3h, filtered through gauze or filter cloth, and the filtrate was collected, concentrated by a rotary evaporator to 50ml, centrifuged at 3500r / min for 10min, collected Transfer the supernatant to a beaker, add 4 times the volume of 95% ethanol while stirring, place at 4°C, alcohol precipitation for 12 hours, centrifuge at 3500r / min for 10 minutes, collect the precipitate, and dry the precipitate to obtain crude polysaccharide. Configure a 5% trichloroacetic acid solution, configure the dried Artemisia argyi crude polysaccharide into a 5% aqueous...
Embodiment 2
[0029] Example 2: Component Separation and Monosaccharide Component Analysis of Folium Artemisiae Argyi Polysaccharides
[0030] 1. Experimental method:
[0031]DEAE sepharose fast flow is initially stored in 20% ethanol solution. After packing the column, it is eluted with distilled water until it has no alcohol smell, washed with 0.5M NaOH for 2 times the column volume, and eluted with distilled water until neutral. Configure distilled water, 0.1M, 0.2M, 0.5M NaCl solution as eluent. The decolorized mugwort leaf polysaccharide solution was filtered with a 0.45uM microporous membrane, loaded on a peristaltic pump, and eluted with distilled water at a flow rate of 1 column volume / h. The elution volume of different eluents was determined according to the elution curve. The eluents of different components were concentrated to 10ml by rotary evaporation, put into a dialysis bag with a cut-off flow rate of 3500Da, dialyzed for 12h, introduced the solution into a petri dish, and v...
Embodiment 3
[0038] Example 3: Effects of Artemisia argyi Polysaccharides and Artemisia Artemisiae Crude Polysaccharides on the Community Structure of the Intestinal Flora in Mice
[0039] The (un)weighted unifrac algorithm was used to obtain the unweighted unifrac index and the weighted unifrac index, and the Wilcox rank sum test was used to compare the differences in the community structure of species in the intestinal flora of different groups of mice. In the comparison of unweighted unifrac index, there is a significant difference between the middle dose group and low dose group of Artemisia argyi and the control group. In addition, there were significant differences between the high-dose group and the middle-dose group and low-dose group respectively ( image 3 A), in the comparison of the weighted unifrac index considering species abundance, there is a significant difference between the Artemisia argyi crude polysaccharide group and the control group ( image 3 B), the above results...
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