Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of TGS1 gene in regulation and control of size and yield of Oryza sativa L. grain

A rice grain, 1. TGS1 technology, applied in the fields of molecular biology and genetic engineering, can solve the problems of new genes to be identified and limited knowledge, and achieve the effects of shortening the breeding cycle, high commercial value, and high yield

Active Publication Date: 2021-12-31
HUAIYIN TEACHERS COLLEGE
View PDF7 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although many molecular pathways and key regulatory factors involved in the regulation of rice grain size have been identified, our understanding of the entire regulatory network is still very limited, and new genes are still to be identified

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of TGS1 gene in regulation and control of size and yield of Oryza sativa L. grain
  • Application of TGS1 gene in regulation and control of size and yield of Oryza sativa L. grain
  • Application of TGS1 gene in regulation and control of size and yield of Oryza sativa L. grain

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1: Method for Knocking Out TGS1 Gene to Increase Rice Grain Size and Yield Based on CRISPR / Cas9 Technology

[0034] The sequence of the gene TGS1 is shown in SEQ ID No.1. Sequence analysis showed that the gene consisted of 3 exons, which were the 1st-287th (1st exon), 988-2512th (2nd exon) and 988th-2512nd (2nd exon) of the sequence of SEQ ID No.1. Positions 2647-3012 (exon 3).

[0035] (1) Construction of TGS1 gene knockout recombinant expression vector

[0036] Design the CRISPR / Cas9 gene knockout target site in the coding region of the TGS1 gene, targeting the antisense strand of the second exon, and the target sequence is shown in SEQ ID No.5. Synthesize target sequence primers based on the CRISPR / Cas9 system, the sequence is as follows:

[0037] TGS1-P1: 5'- TGTG GGCGCCGCCGGCCACCCCTG-3',

[0038] TGS1-P2: 5'- AAAC CAGGGGTGGCCGGCGGCGCC-3',

[0039] The underlined part is the cohesive end used for vector ligation, and the ununderlined part is the tar...

Embodiment 2

[0059] Embodiment 2: Construction of TGS1 overexpression strain

[0060] In order to further verify the function of the gene regulating rice grain size, an overexpression line of the gene was constructed. Use a pair of PCR primers to amplify the complete coding sequence of TGS1, digest it with restriction enzyme Bsa I (NEB), and connect it into the downstream of the ZmUBI promoter of the pEGOEPubi-H vector with hygromycin resistance gene to construct pEGOEPubi- H-TGS1 overexpression transgene vector. The sequence of the pEGOEPubi-H-TGS1 overexpression transgene vector is shown in SEQ ID NO.12. The PCR primer sequences are as follows:

[0061] TGS1OE-F:

[0062] 5'-ACTAG GGTCTC GCACCATGCCGCCATTCTCGGCAGCCG-3',

[0063] TGS1OE-R:

[0064] 5'-ACTAG GGTCTC TACCGTCACTGGACGCAGGCCAAGAATGA-3',

[0065] Wherein the underlined part is the recognition site of Bsa I.

[0066] Referring to the method of Nishimura et al. (Nishimura A, Aichi I, Matsuoka M.A protocol for Agrobacteri...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses application of a TGS1 gene in regulation and control of the size and the yield of Oryza sativa L. grain, and belongs to the technical field of molecular biology and gene engineering. A gene TGS1 coding region knockout target site is designed, a gene knockout mutant is constructed by using a CRISPR / Cas9 technology, and the TGS1 gene in Oryza sativa L. is knocked out. Through offspring separation and detection, a homozygous mutant without transgene insertion is obtained. The grain length, the grain width, the grain thickness and the yield of a single plant of different mutant grains are obviously increased by being compared with those of a control group. In addition, the length-width ratio of the mutant grain is obviously greater than that of a control group, so that the mutant grain has better appearance quality. Therefore, the application of the TGS1 gene provided by the invention in the regulation and the control of the size and the yield of the Oryza sativa L. grain has an important application value, and a good economic benefit can be generated.

Description

technical field [0001] The invention belongs to the technical fields of molecular biology and genetic engineering, and relates to the application of TGS1 gene in regulating rice grain size and yield. Background technique [0002] Rice is one of the most important food crops in the world, and nearly half of the world's population depends on rice as a staple food. Increasing rice production is of great significance to meet the needs of the world's growing population and ensure food security. Rice yield is composed of three major factors: the number of panicles per plant, the number of grains per panicle and the weight of grains. Among them, grain weight is positively correlated with grain size, so grain size is one of the important agronomic traits that determine rice yield. In recent years, a large number of studies through the mapping, cloning and reverse genetics of quantitative trait loci (QTLs) have shown that ubiquitination-mediated proteasomal degradation, plant hormo...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N15/29C12N15/84C12N15/55A01H5/10A01H6/46
CPCC07K14/415C12N15/8218C12N15/8261C12N15/8205C12N9/22
Inventor 高清松赵祥祥周海英李伟张雯霞
Owner HUAIYIN TEACHERS COLLEGE
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com