Quantitative analysis of multi-components by single marker of medicine

A detection method and drug technology, applied in the field of pharmaceutical analytical chemistry, to achieve the effects of good reproducibility, comprehensive information and rapid method

Pending Publication Date: 2022-01-18
NANJING SUZHONG PHARMA RES CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the quality of medicines and medicinal materials selected from Polygonum multiflorum extract, Puhuang extract, Danshen extract, Chuanqiong extract, hawthorn extract, Alisma extract, Codonopsis extract, or Pushen capsule The control method has not been clearly recorded, so it has important practical significance to provide a quality control method for medicines and medicinal materials

Method used

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  • Quantitative analysis of multi-components by single marker of medicine
  • Quantitative analysis of multi-components by single marker of medicine
  • Quantitative analysis of multi-components by single marker of medicine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0094] The one-test and multiple-evaluation detection method of embodiment 1 medicine

[0095] Preparation of reference substance solution: Take the reference substance and accurately weigh gallic acid, stilbene glycoside, chrysophanol-8-O-β-D-glucoside, emodin-8-O-β-D-glucoside, emodin Methyl ether-8-O-β-D-glucoside, aloe-emodin, rhein, emodin, chrysophanol, emodin methyl ether amount, accurately weighed, made by adding methanol containing gallic acid 2.94μg / ml, di Styryl glycoside 15.84μg / ml, Chrysophanol-8-O-β-D-glucoside 6.42μg / ml, Emodin-8-O-β-D-glucoside 7.38μg / ml, Emodin-8 -O-β-D-glucoside 6.6μg / ml, aloe-emodin 6.12μg / ml, rhein 6.30μg / ml, emodin 6.60μg / ml, chrysophanol 6.10μg / ml, emodin methyl ether 6.18μg / ml ml of the mixed reference solution, that is.

[0096] Preparation of the test solution: take the contents of Pushen capsules, grind them finely, take about 1g, accurately weigh them, put them in a stoppered Erlenmeyer flask, add 50ml of 70% ethanol accurately, we...

Embodiment 2

[0102] The one-test and multiple-evaluation detection method of embodiment 2 medicine

[0103] Preparation of the reference substance solution: see Implementation 1 Preparation of the reference substance solution.

[0104] The preparation of need testing solution: see the preparation of implementation 1 need testing solution. A total of 10 batches of Pushen Capsules were tested.

[0105] Instruments and conditions: Thermo Fisher u3000 high performance liquid chromatography, Agilent ZOXBAR SB-C18 chromatographic column, detection wavelength 273nm, column temperature 25°C, flow rate 1.0ml / min, injection volume 10μl, mobile phase 0.1% phosphoric acid solution ( A) - methanol (B), gradient elution.

[0106] The elution program is shown in Table 2.

[0107] Table 2 Example 2 Chromatographic column gradient elution program

[0108]

[0109] Chromatogram see Figure 3-6 . image 3 Peak No. 1 is gallic acid, peak No. 2 is stilbene glycoside, peak No. 3 is chrysophanol-8-O-β-D...

Embodiment 3

[0119] Detection of anthraquinone compounds in the medicinal material of embodiment 3

[0120] Using the detection method in Example 2 of the present invention, it was detected whether each medicinal material of Pushen Capsules contained anthraquinone compounds, and the results are shown in Table 6.

[0121] Table 6 The situation of anthraquinone compounds contained in each medicinal material of Pushen Capsules

[0122]

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Abstract

The invention relates to the field of analytical chemistry, in particular to a quantitative analysis of multi-components by single marker of medicine, which can be used for detecting 10 chemical components of typha angustifolia-ginseng capsules and detecting anthraquinone components in one or more extracts of codonopsis pilosula, ligusticum wallichii, red paeony root, rhizoma alismatis, salvia miltiorrhiza, hawthorn, pollen typhae and polygonum multiflorum. The method is quick, simple, convenient and accurate.

Description

technical field [0001] The invention relates to the field of pharmaceutical analytical chemistry, in particular to a drug detection method with one measurement and multiple evaluations. Background technique [0002] Pushen Capsules are used for blood stasis syndrome of hyperlipidemia. Blood stasis syndrome refers to the syndrome of internal resistance due to blood stasis, with pain, mass, bleeding, purple tongue, astringent pulse, etc. as the main manifestations. Any blood that leaves the meridians, fails to discharge or dissipate in time, but stays in a certain place; or blood circulation is blocked, accumulates in the meridians or organs, and loses physiological functions, all belong to blood stasis. Qi and blood circulation is blocked, and if it is blocked, it will cause pain, stabbing pain, immobility, and refusal to press; the blood flow is slow at night, and the stasis is aggravated, so the pain is aggravated at night; Blood clogs the veins, so that the blood cannot ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/34G01N30/86
CPCG01N30/02G01N30/34G01N30/8675G01N2030/047
Inventor 唐海涛徐杰张琴汪斌陈俊潘小慧葛海涛
Owner NANJING SUZHONG PHARMA RES CO LTD
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