Preparation method and application of proteophilic and plant-philic nitrogen source probiotic solid culture medium
A technology of solid culture medium and probiotics, which is applied in the field of solid culture medium of probiotics probiotics that are proteotropic and phytophilic nitrogen sources, can solve the problem that fermentation propagation technology has not been widely popularized, the popularization of self-cultivation at home has not been realized, and the market price of bacteria can be solved. Expensive and other problems, to achieve the effect of reducing application cost, reducing difficulty and cost, and low circulation cost
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Embodiment 1
[0053] The preparation method of the present embodiment is the following steps:
[0054] (1) Ingredients The proteinophilic and phytophilic nitrogen source probiotic solid medium is composed of nougatose basic ingredients, animal non-dairy non-broth protein nitrogen source and edible fungal nitrogen source, and prebiotic oligosaccharides in fractional ratio; Among them, 60-70% of the basic ingredients of nougat by weight, 10-15% of animal non-dairy and non-broth protein source (cooked dry powder), 10-15% of edible fungal nitrogen source (dry powder), and probiotics Meta oligosaccharides 10-15%;
[0055] (2) Preparation (1) Making sugar with nougat ingredients and preparation methods (2) Adding animal non-dairy and non-broth protein according to the weight to weight ratio when the sugar embryo is cooled to 80-100°C and is in a fluid or semi-fluid state Source (cooked dry powder) and edible fungal nitrogen source (dry powder) and stir or knead to make the added ingredients even...
Embodiment 2
[0068] The present embodiment is the same as Example 1, except that the bird's nest and donkey-hide gelatin are replaced by an equivalent amount of beef or pork floss; the agaricus blazei is used to replace matsutake, and the beef floss is commercially available beef or pork velvet (cooked lean meat fine short fiber) )loose
Embodiment 3
[0070] The preparation method of the present embodiment is the following steps:
[0071] (1) Ingredients malt milk extract (commercially available product), animal non-dairy non-broth protein source and edible fungal nitrogen source are composed according to fractional ratio; among them, malt milk extract is 60-70% by weight and animal non-dairy non-broth protein source Broth protein source (cooked dry powder) 10-15%, edible fungal nitrogen source (dry powder) 10-15%; prebiotic oligosaccharides 10-15%;
[0072](2) Preparation Accurately weigh the four types of powder ingredients in proportion and mix them uniformly, and make a probiotic powder solid culture medium after mixing, and the water content is less than 5%;
[0073] (3) Packaging and storage The powdered solid culture medium prepared in Preparation 2 is subpackaged, packed into the required gram weight, and stored at normal temperature or under dry conditions.
[0074] The concrete batching formula of this embodiment...
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