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Nozzle, liquid drop photo-thermal control system and application thereof

A nozzle and droplet technology, applied in the direction of spray device, spray device, fluid controller, etc., can solve the problems of cross-contamination and leakage, and achieve the effect of easy cleaning and replacement, easy processing, and avoiding the risk of cross-contamination

Active Publication Date: 2022-01-25
HUAZHONG UNIV OF SCI & TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0014] In view of the above defects or improvement needs of the prior art, the present invention provides a nozzle, a liquid droplet photothermal control system and its application, using laser heating to heat up quickly and with a high degree of controllability, and DNA can be detected through the laser heating area in nucleic acid detection. Heat cracking and increase the probability of single nanoparticles in the oil, which is convenient for post-processing, avoids the process of transferring reagents between different equipment, and avoids cross-contamination and leakage of samples

Method used

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  • Nozzle, liquid drop photo-thermal control system and application thereof
  • Nozzle, liquid drop photo-thermal control system and application thereof
  • Nozzle, liquid drop photo-thermal control system and application thereof

Examples

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Embodiment 1

[0092] This embodiment provides an application of a liquid droplet photothermal manipulation system. Specifically include the following steps:

[0093] 1) A liquid droplet photothermal manipulation system, the structure is as attached Figure 5 As shown, using the attached figure 2 The straight nozzle shown has a mesh size of approximately 10 ± 0.5 µm. Use an 808nm, 3000mW near-infrared fiber laser to focus on the nozzle with a strip-shaped spot about 10mm long and 2mm wide. The laser is modulated such that the spot sends an optical signal at a frequency of 2 Hz. Switch on the constant temperature heating sheet until the temperature in the constant temperature zone reaches 65°C. Turn on the pneumatic micropump.

[0094] 2) Switch the inlet reversing valve to the left position, and the PCR reaction reagent and dispersant are respectively injected into the nozzle from the syringe through the one-way valve at a speed ratio of about 3:1.

[0095] The PCR reaction reagent wa...

Embodiment 2

[0103] The difference between this embodiment and embodiment 1 is that the attached figure 1 Curved nozzles shown have the same mesh size.

Embodiment 3

[0105] The difference between this embodiment and embodiment 1 is that the outlet of the curved nozzle uses an additional Figure 3B The double mesh construction shown has the same mesh size.

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Abstract

The invention belongs to the related technical field of nucleic acid detection, and discloses a nozzle, a liquid drop photo-thermal control system and application thereof. The nozzle comprises a first outlet and a plurality of second outlets, the second outlets are formed in the outlet of the first outlet and communicate with one another, a sieve tray is arranged at the outlet of the first outlet and is provided with a plurality of sieve pores; the periphery of the sieve tray is detachably connected with the inner wall of the first outlet in a sealed mode, one end of the sieve tray is communicated with the second outlets, and the other end of the sieve tray is communicated with a laser irradiation area; the laser irradiation area is used for receiving laser irradiation so as to heat the fluid in the first outlet. By adopting laser heating, the temperature rise is rapid, the controllable degree is high, DNA heating pyrolysis can be realized through the laser heating area in nucleic acid detection, the probability of single nanoparticles in oil is improved, the process of transferring reagents among different devices is omitted, and the problems of cross contamination and leakage of samples are avoided.

Description

technical field [0001] The invention belongs to the technical field related to nucleic acid detection, and more specifically relates to a nozzle, a liquid droplet photothermal manipulation system and applications thereof. Background technique [0002] Nucleic acid detection has high sensitivity and high effectiveness, and is the most widely used detection method for pathogen infection. [0003] BEAMing (beads, emulsion, amplification, and magnetics) is a nucleic acid detection technology that combines digital PCR technology and droplet technology, proposed by Devin Dressman et al. in 2003. This technology adds oil and emulsifier to the PCR reaction reagent to form an emulsion with the reaction reagent as the dispersed phase, and each droplet contains at most 1 DNA molecule and 1 magnetic bead. The emulsion is then temperature cycled so that the DNA molecules within each droplet containing both DNA molecules and magnetic beads are individually amplified and adsorbed to the m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01L3/00C12Q1/686B05B1/14B05B1/24
CPCB01L3/502715B01L3/502738C12Q1/686B05B1/14B05B1/24B01L2200/10B01L2400/02C12Q2563/159C12Q2563/155C12Q2563/107
Inventor 刘智勇虞涛菘廖广兰叶伦张许宁何春华甘浪
Owner HUAZHONG UNIV OF SCI & TECH