Preparation method of high-purity aucubin and application thereof

A kind of aucubin, high-purity technology, applied in the field of preparation of high-purity aucubin, can solve problems such as staying in the laboratory stage, achieve the effect of reducing cell growth inhibition rate, improving purity, and preventing decomposition

Active Publication Date: 2022-01-28
INST OF ANALYSIS GUANGDONG ACAD OF SCI (CHINA NAT ANALYTICAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, domestic research on the preparation process of aucubin is still in the laboratory stage, and most of the extraction and preparation processes need to use toxic organic reagents such as petroleum ether, n-butanol, ethyl acetate, etc.

Method used

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  • Preparation method of high-purity aucubin and application thereof
  • Preparation method of high-purity aucubin and application thereof
  • Preparation method of high-purity aucubin and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] A kind of preparation method of high-purity aucubin, such as figure 1 shown, including the following steps:

[0032] (1) Accurately weigh the sample to be tested of Eucommia leaf extract, place it in a flask, and disperse it evenly with distilled water to obtain a 10 mg / mL extract; Dry, keep the target active ingredient as much as possible, extract with ethanol aqueous solution twice, each time for 1h, concentrate, and dry to obtain the test sample of Eucommia ulmoides leaf extract, the content of aucubin is 5.81% as measured by HPLC;

[0033] (2) The pretreated HPD-100, HPD-300, HPD-600, D-3250, X-5, D-140, NKA-9, NKA-II, D-101, AB-8, S- 8 and polyamide resin were mixed with the extract at a material-to-liquid ratio of 1:50 (g:mL), shaken at 25°C to reach adsorption equilibrium, and the eluate was collected in sections, and the elution was determined by the p-dimethylaminobenzaldehyde method. The content of aucubin in the solution; then the resin was rinsed three tim...

Embodiment 2

[0037] A kind of preparation method of high-purity aucubin, such as figure 1 shown, including the following steps:

[0038] (1) Accurately weigh the test sample of Eucommia bark extract, place it in a flask, and disperse it evenly with distilled water to obtain a 10mg / mL extract; the described Eucommia bark extract is fresh skin slices, 30~50°C Drying, keeping the target active ingredient as much as possible, extracting with ethanol-water solution twice, each time for 1 hour, concentrating, and drying to obtain the test sample of Eucommia bark extract, the content of aucubin was 8.84% as measured by HPLC;

[0039](2) Pack the pretreated HPD-100 resin into the column, put the extract on the column (150mL), place it at a constant temperature for 60 minutes until the adsorption equilibrium is reached, and use 40% ethanol aqueous solution for elution, and the solution volume is 4BV , the flow rate is 3BV / h, the eluate is collected in sections, and concentrated under reduced press...

Embodiment 3

[0042] A kind of preparation method of high-purity aucubin, such as figure 1 shown, including the following steps:

[0043] (1) Accurately weigh the sample to be tested of Eucommia leaf extract, place it in a flask, and disperse it evenly with distilled water to obtain a 10 mg / mL extract; Dry, keep the target active ingredient as much as possible, extract with ethanol solution twice, each time for 1h, concentrate, and dry to obtain the test sample of Eucommia ulmoides leaf extract, the content of aucubin is 5.99% as measured by HPLC;

[0044] (2) Pack the pretreated X-5 resin into a column, put the extract on the column (150mL), place it at a constant temperature for 120min to achieve adsorption equilibrium, and use 40% ethanol aqueous solution for elution, the solution volume is 3BV, The flow rate is 2BV / h, the eluate is collected in sections, and concentrated under reduced pressure at 50°C to obtain a concentrated solution;

[0045] (3) The concentrated solution of the abo...

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Abstract

The invention discloses a preparation method of high-purity aucubin and an application thereof. The preparation method comprises the following steps: uniformly dispersing a cortex eucommiae extract with distilled water to obtain an extracting solution, packing screened and pretreated resin into a column, loading the extracting solution into the column, eluting with an ethanol water solution, collecting eluent in sections, measuring, and concentrating under reduced pressure to obtain a concentrated solution; injecting the concentrated solution into a medium-pressure preparative liquid chromatograph for preparative separation, performing vacuum concentration and vacuum freeze drying, and obtaining the high-purity aucubin. According to the method, the macroporous adsorption resin and the medium-pressure preparative liquid chromatograph are combined, through the synergistic effect of the macroporous adsorption resin and the medium-pressure preparative liquid chromatograph, the purity of the prepared aucubin sample is greatly improved, meanwhile, ethanol is used for replacing toxic organic reagent eluent, the method is harmless to the environment, the macroporous resin can be repeatedly used, and the production cost is lower. The prepared product has anti-aging activity and good safety, and can be applied to preparation of anti-aging cosmetics or health care products.

Description

technical field [0001] The invention relates to the technical field of plant extraction, in particular to a preparation method and application of high-purity aucubin. Background technique [0002] Eucommia ulmoides (Eucommia ulmoides) is a plant of the genus Eucommia in the Eucommia family. Its bark and leaves are recorded in the "Pharmacopoeia of the People's Republic of China", and it is recorded that it has the effects of nourishing the liver and kidney, strengthening bones and bones, and preventing miscarriage. Modern pharmacological studies have shown that Eucommia also has the effects of lowering blood pressure, lowering blood fat, lowering blood sugar, enhancing immunity, anti-oxidation, antibacterial, and delaying aging. It has high scientific research value, health care medicinal value and economic development value. Through the chemical analysis of a large number of Eucommia leaves, bark and extracts, it is found that the content of chlorogenic acid in Eucommia is ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H1/08C07H17/04A61K8/60A61Q19/08A23L33/105
CPCC07H1/08C07H17/04A61K8/602A61Q19/08A23L33/105A23V2002/00A23V2200/302A23V2250/21
Inventor 彭密军杨秋玲王志宏王雪松张淑欣
Owner INST OF ANALYSIS GUANGDONG ACAD OF SCI (CHINA NAT ANALYTICAL
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