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Application of o-hydroxycinnamic acid of in removal of harmful benthic diatom

A technology of o-hydroxycinnamic acid and benthic diatoms, applied in the fields of application, herbicides and algicides, seaweed cultivation, etc., can solve problems such as diatoms cannot be fundamentally removed, environmental damage is great, and secondary pollution , to achieve the effect of saving manpower, low price and easy degradation

Pending Publication Date: 2022-02-01
GUANGDONG OCEAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The physical removal method requires a lot of manpower and time, and the cost is relatively high, and it cannot fundamentally remove diatoms
Although the chemical removal method is effective, it is easy to cause serious secondary pollution and cause great damage to the environment

Method used

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  • Application of o-hydroxycinnamic acid of in removal of harmful benthic diatom
  • Application of o-hydroxycinnamic acid of in removal of harmful benthic diatom
  • Application of o-hydroxycinnamic acid of in removal of harmful benthic diatom

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The acquisition of embodiment 1 o-hydroxycinnamic acid

[0027] O-hydroxycinnamic acid is extracted from Gracilaria isobranchus. Gracilaria heterochia was collected from Fengjiawan, Wenchang City, Hainan Province (19°24′49″N, 110°42′53″E). Take 20mg of dry powder sample of Gracilaria isobrachiae into a 1.5mL centrifuge tube, add 400μL of extract solution (acetonitrile:methanol=1:1, volume ratio), vortex and mix for 30s, then extract with low-temperature ultrasound for 30min (5°C, 40KHz), Place the sample at -20°C for 30 minutes, centrifuge at 13,000g at 4°C for 15 minutes, remove the supernatant, dry it with nitrogen, add 120 μL of complex solution (acetonitrile: water = 1:1, volume ratio) to redissolve, and extract with low-temperature ultrasonic 5min (5°C, 40KHz), centrifuge at 4°C, 13000g for 5min, pipette the supernatant into a vial with an inner insert for mass spectrometry analysis, sample preparation design 6 parallel. Take an equal volume of all sample metabol...

Embodiment 2

[0028] Embodiment 2 A kind of method of removing adsorption type benthic diatoms (taking Nitzschia crescentus as model)

[0029] (1) Choose a PE plastic square water tank without a cover with a water capacity of 600L. After the tank wall is scrubbed, the tank wall is repeatedly splashed with a potassium permanganate solution with a concentration of 5 mg / L, soaked for 10 minutes, and then disinfected with seawater. Rinse well.

[0030] (2) Inject fresh sand-filtered seawater into the cleaned water tank, cover the water inlet with a 500-mesh filter bag to prevent the entry of harmful organisms, and fill the water to 500L. Disinfect the seawater with 50mL 30% available chlorine bleach solution and let it stand for 6 hours. Before seawater is used, add 9.24g of sodium thiosulfate for neutralization and fully aerate for 3 hours.

[0031] (3) Add o-hydroxycinnamic acid dissolved in 0.1% dimethyl sulfoxide to the water tank with sterilized seawater, so that the final concentration ...

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PUM

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Abstract

The invention belongs to the technical field of seaweed cultivation, and particularly relates to an application of o-hydroxycinnamic acid in removal of harmful benthic diatom. The o-hydroxycinnamic acid is used as an algicide to remove the harmful benthic diatom, and the algicide-o-hydroxycinnamic acid used in the invention is an extract of gracilaria bailinae. Compared with a traditional diatom removal method, the application has the following remarkable advantages: (1) the diatom removal agent is easy to degrade in a natural environment and does not generate secondary pollution; (2) the effect is quick, the inhibition rate on adsorption type benthic diatom after 2 days, under the treatment of 3 mM / L o-hydroxycinnamic acid, exceeds 50%, operations are simple, and the manpower is saved; (2) the price is low and the acquisition is easy; therefore, the method for removing the harmful benthic diatom is an environment-friendly, efficient, and economical method of benthic diatom prevention and removal.

Description

technical field [0001] The invention belongs to the technical field of seaweed cultivation, and in particular relates to the application of o-hydroxycinnamic acid in removing harmful benthic diatoms. Background technique [0002] Diatoms are a class of single-celled microalgae with siliceous shells. There are as many as 250 genera and 100,000 species in various species, and they can be connected into various groups through multiple individuals. The distribution range of diatoms is extremely wide, and there are a large number of distributions from fresh water to sea water. In the marine environment, diatoms, as the most important primary producers, can constitute 20% of the primary productivity on a global scale, and are also important participants in the global silicon cycle and carbon cycle. According to different habitat conditions, diatoms can be divided into planktonic diatoms and benthic diatoms. Among them, benthic diatoms are the most basic part of marine biofouling...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01M21/04A01G33/00A01N37/38A01P13/00
CPCA01M21/043A01G33/00A01N37/38Y02A40/80
Inventor 谢恩义黄博文崔建军黄永健陈心怡
Owner GUANGDONG OCEAN UNIVERSITY
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