Extraction method of peony isolated protein with high emulsibility and oil absorbency

Abstract

The invention relates to an extraction method of a peony isolated protein with high emulsibility and oil absorbency, wherein the method comprises the following steps: 1, extracting peony seeds for hulling oil at low temperature by using butane or extracting the peony seeds for hulling oil at low temperature by using normal hexane or extracting the peony seeds for hulling oil by using supercritical fluid or extracting the peony seeds for hulling oil by using butane or normal hexane or supercritical fluid, and obtaining degreased peony meal with the residual oil rate of less than or equal to 2.5%; 2, carrying out alcohol extraction on the degreased peony seed meal by using methanol or ethanol as a solvent to remove small molecular substances such as flavone and paeoniflorin in the peony meal; 3, carrying out alkali extraction on the degreased peony meal subjected to alcohol extraction in a NaOH solution, centrifuging, adjusting the pH value of the supernate to 3-5 by using diluted hydrochloric acid, centrifuging, and adjusting the pH value of the precipitate to 6.8-7.4 by using the NaOH solution, so as to obtain a neutral protein emulsion; and 4, carrying out flash evaporation heat treatment on the neutral protein emulsion at the temperature of 125-135 DEG C for 5-15 seconds, and carrying out spray drying to obtain the peony isolated protein with high emulsibility and oil absorbency. According to the method, the protein which is adjusted to be neutral after alkali extraction and acid precipitation is subjected to flash evaporation heat treatment, and the peony isolated protein with high emulsibility and oil absorbency is obtained in the step.

Description

technical field [0001] The invention belongs to the research field of performance improvement of peony protein isolate, in particular to a method for extracting high emulsifying and oil-absorbing peony protein isolate. Background technique [0002] Peony for oil is a general term for species with a seed-yielding rate of ≥22% in the peony group. It is an emerging woody oil crop in my country, mainly including Fengdan peony and purple spot peony. 60-70% cakes are produced after oil extraction from peony seeds. These cakes are only used as feed or waste, resulting in waste of resources. The cakes contain a large amount of active substances such as peony protein, peony flavonoids, peony polysaccharides, and paeonol. . Among them, the protein content accounts for about 20-30% of the weight of the cake, the amino acid composition is balanced, and it has extremely high nutritional value. At present, the extraction and development of peony protein adopts the traditional alkali ext...

AI Technical Summary

Problems solved by technology

Because the functionality of soybean protein is far worse than that of casein, there is no industrialized case of plant protein replacing casein (animal protein) to prepare non-dairy creamer. However, since the cost of casein is much higher than that of plant protein, plant protein is used Replacing casein is an important direction of current research. In the early stage, we published the patent CN202110752411.6 "A Method for Extracting Peony Protein Isolate for Oil". Through this method, the color of peony protein was improved, and the maximum improvement of peony protein was achieved. Extraction rate and purity, but poor functional properties, its water absorption, oil absorption, emulsification, emulsion stability, foaming and foam stability are only slightly higher than soy protein isolate, far inferior to casein

Although it has extracted peony protein, its functionality is not enough to replace casein. How to improve the performance of peony protein isolate so that it can replace casein, thereby reducing production costs, has become a major problem that plagues the in-depth research of peony protein isolate

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