High-efficiency valine semi-continuous fermentation method and complete equipment thereof
A valine, semi-continuous technology, applied in the field of high-efficiency valine semi-continuous fermentation method and its complete set of equipment, can solve the difficulty of increasing the post-treatment of yeast liquid, production cost, low utilization rate of fermentation equipment and bacterial liquid , high residual glucose and other issues, to achieve the effect of improving equipment utilization, shortening the fermentation cycle, and improving product quality
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Embodiment 1
[0066] The cultivation method of fermented seed liquid among the embodiment 1:
[0067] Valine-producing bacteria (preservation number: CGMCC No.19457) were inoculated into LB medium according to the inoculum size of 1-5%, and the culture system was placed at 180-220r / min, 35-37°C, pH 6- Under the condition of 7, cultivate to OD value 3-4 to obtain the primary seed solution; transfer the primary seed solution to the synthetic medium according to the inoculation amount of 1-5%, and place it in 180-220r / min, 35- Under the conditions of 37°C and pH 6-7, cultivate to OD value 3-4 to obtain fermented seed liquid, which is used in Example 1.
Embodiment 2
[0068] The cultivation method of fermented seed liquid in embodiment 2:
[0069] Valine-producing bacteria (preservation number: CGMCC No.19456) were inoculated into LB medium according to the inoculum size of 1-5%, and the culture system was placed at 180-220r / min, 35-37°C, pH 6- Under the condition of 7, cultivate to OD value 3-4 to obtain the primary seed solution; transfer the primary seed solution to the synthetic medium according to the inoculation amount of 1-5%, and place it in 180-220r / min, 35- Under the conditions of 37°C and pH 6-7, cultivate to OD value 3-4 to obtain fermented seed liquid, which is used in Example 2.
[0070] Valine semi-continuous fermenter
[0071] See attached figure 1 , a semi-continuous fermenter 10 of valine, comprising an inlet pipe (11), a feed pipe (12), a discharge pipe (13), a stirring device (14), a driving device (15), an observation window (16 ), the feed pipe (12) is arranged on the top of the fermentor (10), and the body length o...
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