Sludge recycling method and biological water purification particles
A biological water purification and sludge technology, applied in biological water/sewage treatment, water/sludge/sewage treatment, microorganisms, etc. The mud has not been properly treated and other problems, to achieve good microbial load effect, high-efficiency treatment, and good adsorption effect
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Embodiment 1
[0042] A method for recycling sludge, specifically as follows:
[0043] 1. Using sludge and sewage for strain screening
[0044] (1) Enrichment of strains
[0045] Take a 250ml Erlenmeyer flask, add 90ml of sterilized enrichment medium, then add 10ml of river bottom sludge and sewage to be treated, place in a constant temperature shaking incubator at 180r / min, and incubate at 30°C for 2 days Finally, discard the supernatant, add sterilized enrichment medium to enrich again, repeat the same process and enrich repeatedly 6 times to obtain a high-concentration strain enrichment solution.
[0046] (2) Isolation of strains
[0047] Under sterile conditions, use the 10-fold dilution method to evenly spread the enrichment solution of the strains on the actinomycetes plate medium, LB plate medium and fungal plate medium, seal the plate with sealant, turn the plate upside down, and place into a 30°C constant temperature incubator. Observe the colony morphology on the gradient plate...
Embodiment 2
[0073] A method for recycling sludge, specifically as follows:
[0074] 1. Using sludge and sewage for strain screening
[0075] (1) Enrichment of strains
[0076] Take a 250ml Erlenmeyer flask, add 90ml of sterilized enrichment medium, then add 10ml of river bottom sludge and sewage to be treated, place in a constant temperature shaking incubator at 180r / min, and incubate at 30°C for 2 days Finally, discard the supernatant, add sterilized enrichment medium to enrich again, repeat the same process and enrich repeatedly 6 times to obtain a high-concentration strain enrichment solution.
[0077] (2) Isolation of strains
[0078] Under sterile conditions, use the 10-fold dilution method to evenly spread the enriched solution of the strains on the actinomycetes plate medium, LB plate medium and fungus plate medium, seal the plate with sealant, turn the plate upside down, and place into a 30°C constant temperature incubator. Observe the colony morphology on the gradient plate a...
Embodiment 3
[0104] A method for recycling sludge, specifically as follows:
[0105] 1. Using sludge and sewage for strain screening
[0106] (1) Enrichment of strains
[0107] Take a 250ml Erlenmeyer flask, add 90ml of sterilized enrichment medium, then add 10ml of river bottom sludge and sewage to be treated, place in a constant temperature shaking incubator at 180r / min, and incubate at 30°C for 2 days Finally, discard the supernatant, add sterilized enrichment medium to enrich again, repeat the same process and enrich repeatedly 6 times to obtain a high-concentration strain enrichment solution.
[0108] (2) Isolation of strains
[0109] Under sterile conditions, use the 10-fold dilution method to evenly spread the enriched solution of the strains on the actinomycetes plate medium, LB plate medium and fungus plate medium, seal the plate with sealant, turn the plate upside down, and place into a 30°C constant temperature incubator. Observe the colony morphology on the gradient plate a...
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