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Lactic acid-producing streptococcus bovis and separation method thereof

An isolation method, the technology of Streptococcus bovis, applied in the field of lactic acid-producing Streptococcus bovis and its isolation, can solve the problems of inconvenient separation and low purity, and achieve the effect of improving efficiency, good effect and speeding up the process

Pending Publication Date: 2022-02-15
TARIM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the deficiencies of the prior art, the present invention provides a lactic acid-producing Streptococcus bovis and its isolation method, which solves the problems of inconvenient separation and low purity

Method used

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Examples

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Embodiment 1

[0028] An embodiment of the present invention provides a lactic acid-producing Streptococcus bovis, which is obtained from feces of cows.

[0029] A method for isolating lactic acid Streptococcus bovis comprising the following steps:

[0030] S1: Preparation of raw materials

[0031] Take 100 grams of normal cow feces, dilute it, filter impurities, leave the filtrate, put it into a sterile anaerobic sealed bag and seal it;

[0032] S2: Prepare culture medium

[0033] Using water as the solute, add the following parts by mass: 2.0 grams of soluble starch, 10 grams of glucose, 0.5 grams of dipotassium hydrogen phosphate, 3 grams of calcium carbonate, 0.2 grams of magnesium sulfate, 0.4 grams of yeast powder, 20 grams of agar, 1% 1 ml of crystal violet solution, the specific preparation method is: put the starch in a beaker, make a paste with 5 ml of water, pour 95 ml of water, stir well, add other components to dissolve it, heat to boil Add agar and keep stirring. After the a...

Embodiment 2

[0041] An embodiment of the present invention provides a lactic acid-producing Streptococcus bovis, which is obtained from feces of cows.

[0042] A method for isolating lactic acid Streptococcus bovis comprising the following steps:

[0043] S1: Preparation of raw materials

[0044] Take 100 grams of normal cow feces, dilute it, filter impurities, leave the filtrate, put it into a sterile anaerobic sealed bag and seal it;

[0045] S2: Prepare culture medium

[0046] With water as the solute, add the following parts by mass: 3.0 grams of soluble starch, 20 grams of glucose, 1.0 grams of dipotassium hydrogen phosphate, 4 grams of calcium carbonate, 0.3 grams of magnesium sulfate, 0.5 grams of yeast powder, 30 grams of agar, 1% 1 ml of crystal violet solution, the specific preparation method is: put the starch in a beaker, make a paste with 5 ml of water, pour 95 ml of water, stir well, add other components to dissolve it, heat to boil Add agar and keep stirring. After the ag...

Embodiment 3

[0054] An embodiment of the present invention provides a lactic acid-producing Streptococcus bovis, which is obtained from feces of cows.

[0055] A method for isolating lactic acid Streptococcus bovis comprising the following steps:

[0056] S1: Preparation of raw materials

[0057] Take 100 grams of normal cow feces, dilute it, filter impurities, leave the filtrate, put it into a sterile anaerobic sealed bag and seal it;

[0058] S2: Prepare culture medium

[0059]With water as the solute, add the following parts by mass: 4.0 grams of soluble starch, 30 grams of glucose, 1.5 grams of dipotassium hydrogen phosphate, 5 grams of calcium carbonate, 0.4 grams of magnesium sulfate, 0.6 grams of yeast powder, 40 grams of agar, 1% 1 ml of crystal violet solution, the specific preparation method is: put the starch in a beaker, make a paste with 5 ml of water, pour 95 ml of water, stir well, add other components to dissolve it, heat to boil Add agar and keep stirring. After the aga...

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Abstract

The invention provides lactic acid-producing streptococcus bovis and a separation method thereof, and relates to the technical field of streptococcus bovis separation. The separation method of the lactic acid-producing streptococcus bovis comprises the following steps: S1, preparation of raw materials: taking 100 grams of normal cattle manure, diluting the normal cattle manure, filtering out impurities, leaving a filtrate, filling a sterile anaerobic sealing bag with the filtrate, and sealing the sterile anaerobic sealing bag; and S2, preparation of a culture medium: with water as a solute, adding, in parts by mass, 2.0 to 4.0 grams of soluble starch, 10 to 30 grams of glucose, 0.5 to 1.5 grams of dipotassium phosphate, 3 to 5 grams of calcium carbonate, 0.2 to 0.4 gram of magnesium sulfate, 0.4 to 0.6 gram of yeast powder, 20 to 40 grams of agar and 1 milliliter of a crystal violet solution with a concentration of 1% into the water. According to the invention, starch and agar are added into common culture medium components, so the growth rate of streptococcus bovis can be increased and is higher than the growth rates of other bacteria, rapid proliferation can be achieved, dominant flora can be formed, and follow-up separation is easier; and the crystal violet solution is added into the common culture medium components, so colors are easy to distinguish, and the streptococcus bovis can be quickly selected from other bacteria.

Description

technical field [0001] The invention relates to the technical field of isolation of Streptococcus bovis, in particular to a lactic acid-producing Streptococcus bovis and an isolation method thereof. Background technique [0002] Streptococcus bovis is a normal flora in the digestive tract of herbivores. It is commonly found in the gastrointestinal tract and feces of cattle. isolated from the tract and feces. Streptococcus bovis is also a common flora in the human gastrointestinal tract and can be isolated from human gastrointestinal tract, feces, and food. Therefore, it has long been believed that Streptococcus bovis is a harmless bacterium in the gastrointestinal tract of many animals and humans. However, in 1945, Mc-Neal and Blevins first reported that Streptococcus bovis can cause infective endocarditis. Most patients with endocarditis had valve abnormalities or repaired valves before the onset of the disease. Patients with S. bovis sepsis or endocarditis often have i...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/02C12N1/04C12R1/46
CPCC12N1/20C12N1/02C12N1/04
Inventor 吴静齐萌陈荣
Owner TARIM UNIV
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