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Microbial marker related to norovirus infectious diarrhea and application thereof

A virus infection and microbiological technology, applied in the direction of microbiology, microbiological-based methods, microbiological determination/testing, etc., to achieve good diagnostic efficiency, high specificity, and high sensitivity

Active Publication Date: 2022-02-15
中华人民共和国台州海关
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although there are no drugs and vaccines for the treatment and prevention of norovirus infection, knowing more about the molecular mechanism of the interaction between viruses and bacteria will provide new ideas for the prevention and treatment of enteroviruses, so as to provide a new method for norovirus infection in the field. Microbial markers for early diagnosis and early treatment, so as to realize early detection, early intervention and early treatment of norovirus infection

Method used

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  • Microbial marker related to norovirus infectious diarrhea and application thereof
  • Microbial marker related to norovirus infectious diarrhea and application thereof
  • Microbial marker related to norovirus infectious diarrhea and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Example 1 Detection of Microflora Associated with Norovirus Infectious Diarrhea

[0054] 1. Collection of samples

[0055] The research objects collected in this study include: 35 cases of children with norovirus infectious diarrhea (norovirus infection group, NV group), 25 cases of healthy children (healthy control group, NOR group), excluding antibiotics, probiotics, After taking Chinese herbal medicine and other substances that may affect the structure of the intestinal flora, a total of 31 stool samples from children with norovirus infectious diarrhea and 25 stool samples from healthy children were collected. The stool samples were stored at -80°C for DNA extraction, sequencing and bioinformatic analysis.

[0056] 2. 16S rRNA sequencing

[0057] (1) Extraction of DNA

[0058] After the genomic DNA was extracted, the extracted genomic DNA was detected by 1% agarose gel electrophoresis.

[0059] (2) PCR amplification

[0060] Synthesize specific primers with barc...

Embodiment 2

[0117] Example 2 evaluates and analyzes the diagnostic efficacy of microbial markers

[0118] 1. Model prediction analysis and detection of microbial marker diagnostic efficacy

[0119]Random forest (Random Forest) is used for analysis. The random forest belongs to the machine learning algorithm and is a classifier containing multiple decision trees. Its classification results are based on the attributes of each dimension of the detection sample on different decision trees Make a judgment, and give the final classification after comprehensively considering all the judgment results. For the classification problem, the maximum probability is taken, and the regression analysis takes the probability mean. It can efficiently and quickly select the most important species category (biomarker) for sample classification. Software: R (randomForest package), using random forest, setting 500 decision trees, classification level is species, and sorting by importance. For the sorted specie...

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Abstract

The invention discloses a microbial marker related to norovirus infectious diarrhea and an application thereof, the microbial marker is g_Intestinibacter, g_UBA1819 and g_Lachnospiraceae_FCS020_group, whether a subject suffers from norovirus infectious diseases or not can be predicted or diagnosed by detecting the content or abundance of the microbial marker in a sample of the subject, and meanwhile, the content or abundance of the microbial marker in the sample of the subject can be detected by detecting the content or abundance of the microbial marker in the sample of the subject. The invention also provides an application of the microbial marker in treatment of norovirus infectious diarrhea diseases.

Description

technical field [0001] The present invention belongs to the technical field of biomedicine and molecular biology, and specifically relates to microbial markers related to norovirus infectious diarrhea and uses thereof, more specifically, to microbial markers g_Intestinibacter and g_UBA1819 related to norovirus infectious diarrhea , or g_Lachnospiraceae_FCS020_group and its uses. Background technique [0002] Norovirus (NoV) infectious diarrhea is diarrhea caused by Norovirus infection. The main clinical manifestations are acute gastroenteritis symptoms such as diarrhea, nausea, vomiting, abdominal pain and fever. It has an acute onset and rapid transmission speed. , Involving a wide range of characteristics. Norovirus is one of the main pathogens causing epidemic and self-limiting acute gastroenteritis, often causing serious public health events, and the infection cases account for 18% of the total number of acute gastroenteritis cases in the world (Ahmed S M, Hall A J, Ro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/689C12Q1/02G01N33/569A61K45/00A61P1/12A61P31/14C12R1/01
CPCC12Q1/689C12Q1/025G01N33/56911A61K45/00A61P1/12A61P31/14G01N2469/10G01N2800/065G01N2800/26
Inventor 常佩亮翁永青郑笑徐佳妮郑辉江飞剑李杰徐意王英姿
Owner 中华人民共和国台州海关
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