Method for extracting punicalagin from pomegranate rind

Abstract

The invention discloses a method for extracting punicalagin from pomegranate rind. The method comprises the following steps: (1) preparing pomegranate rind pulp; (2) mixing the pomegranate rind pulp with water, carrying out flash extraction, centrifuging an extract, and taking a supernate; and (3) removing polysaccharide in the supernate, and carrying out freeze-drying to obtain the punicalagin. According to the method, the stability and yield of punicalagin are comprehensively considered, fresh pomegranate rind is taken as a research object, and punicalagin in the pomegranate rind is extracted by adopting a flash extraction method. The punicalagin extraction method established by the invention is simple and convenient to operate, short in extraction time, low in cost and small in pollution, the obtained punicalagin is not hydrolyzed and high in yield, and the punicalagin content in the extract is 615.546 + / - 3.04% mg / g; and the method effectively improves the utilization rate of the punicalagin.

Description

technical field [0001] The invention relates to a method for extracting plant polyphenols, in particular to a method for extracting punicalagin from pomegranate peel, and belongs to the field of punicalagin extraction. Background technique [0002] Pomegranate peel is the main processing by-product of pomegranate. Among them, punicalagin is the main component of pomegranate peel polyphenol, which has the characteristics of high degree of hydroxylation, safety and non-toxicity, and has high development value and health function application value. It has been reported that punicalagin has various pharmacological effects such as antioxidant, anticancer, antibacterial, antiviral, and anti-inflammatory. [0003] So far, the extraction techniques of punicalagin mainly include solvent thermal reflux, immersion and ultrasonic extraction, etc. Most of the extraction solvents are organic solvents, and there are also a small amount of reports of water extraction. Among them, the Chin...

AI Technical Summary

Problems solved by technology

Among them, the Chinese patent whose publication number is CN103467534A discloses a method for extracting punicalagin from pomegranate peel by backflowing dried pomegranate peel as a raw material, which has the disadvantage of time-consuming; the Chinese patent whose publication number is CN104327131A discloses a high-pressure extraction The method for punicalagin in pomegranate peel mainly has the problem of high cost; the Chinese patent publication number CN101747388A discloses a method for simultaneously extracting punicalagin and ellagic acid from pomegranate peel. The main disadvantage of this method is that : the operation is cumbersome, and the use of organic solvent is large; the Chinese patent with the publication number CN201610740838A discloses a method for ethanol extraction of punicalagin from pomegranate peel, and the main disadvantage of this method is that the amount of organic solvent used is large

The Chinese patent with publication number CN101955500A discloses a method for ethanol ultrasonic extraction of punicalagin from pomegranate peel. The main disadvantages of this method are: high energy consumption and low yield of punicalagin

[0004] To sum up, at present, the existing extraction technology of punicalagin from pomegranate peel has the disadvantages of solvent residue, high cost, instability of punicalagin, low yield of punicalagin, difficulty in industrialization, etc., and needs to be improved

Images