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Method for producing whole wheat sour dough leavening agent by utilizing strain synergistic enzyme treatment technology

A technology for sourdough and enzyme treatment, which is applied in the field of leavening agents, can solve the problem that the improvement effect of sourdough starter whole wheat flour fermentation products is not obvious, the palatability, texture characteristics and popularization of whole wheat products are affected, and the degradation effect of insoluble cellulose is affected. Insignificant and other problems, to achieve the effect of facilitating quality control, improving nutritional properties and antioxidant properties, and improving palatability

Active Publication Date: 2022-03-01
HENAN UNIVERSITY OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006](1) The prior art lacks a whole wheat sourdough starter aimed at the characteristics of whole wheat flour and improving the texture and nutritional properties of whole wheat products;
[0007] (2) The temperature of the drying treatment is too high, which will lead to insufficient number of live microorganisms in the sourdough produced, which will greatly affect the fermentation capacity of the starter. The prior art is To protect the bacteria, a protective agent needs to be added when drying, and the sourdough starter produced cannot be stored for a long time, and the activity of the bacteria is low, and most of them need to be made and used now;
[0008](3) The effect of single lactic acid bacteria fermentation treatment on the modification of bran in whole wheat flour is not high, and the sourdough starter produced has little effect on the improvement of whole wheat flour fermented products Obviously, it has no significant effect on the degradation of insoluble cellulose, which affects the palatability, texture characteristics and promotion of whole wheat products

Method used

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  • Method for producing whole wheat sour dough leavening agent by utilizing strain synergistic enzyme treatment technology
  • Method for producing whole wheat sour dough leavening agent by utilizing strain synergistic enzyme treatment technology
  • Method for producing whole wheat sour dough leavening agent by utilizing strain synergistic enzyme treatment technology

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Experimental program
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Effect test

Embodiment 1

[0037] Production of whole-wheat sourdough starter with cellulase xylanase by strains and comparative analysis of its quality.

[0038] Lactobacillus brevis and Saccharomyces cerevisiae were cultured after the primary activation, and then the lactic acid bacteria were re-inoculated and cultivated according to the inoculation amount of 2% (v / v) and yeast according to 5% (v / v) to obtain a bacterial suspension. The two bacterial suspensions were centrifuged at a speed of 5000r / min for 10min, the culture solution in the upper layer was discarded, and the sludge sediment in the lower layer was washed with 20mL of 0.85% normal saline and centrifuged twice to obtain the sludge sediment. Whole wheat flour 100g, water 65g, cellulase and pentosanase additions were 40mg / g and 60mg / g, and the initial inoculum of lactic acid bacteria was 10 9 CFU / g whole wheat flour, the initial inoculum of yeast was 1010 CFU / g whole wheat flour, mixed well, and fermented at 37°C and 85% humidity for 12 ho...

Embodiment 2

[0048] Lactobacillus plantarum and saccharomyces were cultured after the primary activation, and then the lactic acid bacteria were re-inoculated and cultured at an inoculum size of 2% (v / v) and saccharomyces at 5% (v / v) to obtain a bacterial suspension. The bacterial suspension was centrifuged at a speed of 5000r / min for 10min, the upper culture solution was discarded, and the lower bacterial sludge was washed with 20mL of 0.85% normal saline and centrifuged twice to obtain the bacterial sludge. Whole wheat flour 100g, water 65g, cellulase and pentosanase additions were 40mg / g and 60mg / g, and the initial inoculum of lactic acid bacteria was 10 8 CFU / g whole wheat flour, the initial inoculum of yeast was 10 10 CFU / g whole wheat flour, mixed well, and fermented at 37°C and 85% humidity for 12 hours; adding 400% sterile saline, followed by low-temperature spray drying to obtain a whole wheat sourdough starter. The spray drying conditions are inlet temperature 130°C; outlet temp...

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Abstract

The invention discloses a method for producing a whole wheat sour dough leavening agent through cooperation of strains and exogenous enzymes. The method comprises the following steps: (1) activation culture of lactic acid bacteria and saccharomycetes fermentation strains; (2) centrifugally precipitating the bacterial suspension to prepare fermented bacterial sludge; (3) mixing a certain amount of bacterial sludge precipitate, water and exogenous enzyme to prepare whole wheat flour fermentation paste; (4) after fermentation, adding the sterile normal saline and the strain protection liquid again, and homogenizing; (5) carrying out low-temperature spray drying on the whole wheat fermented paste after homogenization; and (6) collecting the whole-wheat fermented sour dough bacterial powder and packaging the whole-wheat fermented sour dough bacterial powder. After being stored for a long time, the prepared whole wheat sour dough leavening agent still has high viable count and enzyme activity, the storage stability is improved, the soluble arabinoxylan content of a whole wheat product produced through fermentation is improved, the oxidation resistance and the mineral ion absorption capacity of the whole wheat product are obviously improved, and the whole wheat sour dough leavening agent is suitable for being used as a whole wheat sour dough leavening agent. The nutritional value, the texture and the sensory quality are obviously improved.

Description

technical field [0001] The invention belongs to the technical field of starters, and in particular relates to a method for producing whole wheat sourdough starters by utilizing the co-enzyme treatment technology of lactic acid bacteria and saccharomyces through low-temperature spray drying technology. Background technique [0002] Fermented flour products have a long history. In my country, there are steamed buns, steamed buns, flower rolls, etc., and in foreign countries, there are fermented noodle cakes, steamed buns and other products. Fermented flour products are fragrant and delicious, soft and pleasant, and have been widely loved. The conventionally used leavening agents are mainly yeast and sourdough (ferment) used by a small number of families, but the fermented products produced by dry yeast fermentation have insufficient flavor, and people prefer to choose a starter with richer flavor and higher nutrition, sourdough It is one of the common leavening agents, but sou...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/16A23L7/104C12R1/24C12R1/25C12R1/225C12R1/645
CPCC12N1/20C12N1/16A23L7/104A23V2002/00A23V2400/121A23V2400/183A23V2400/123A23V2400/169A23V2250/762
Inventor 洪静郑学玲刘翀李利民张书庆
Owner HENAN UNIVERSITY OF TECHNOLOGY
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