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Preparation method of silver ion catalytic western blot optical and electrochemical sensor

A Western blotting, silver ion technology, applied in the field of functional materials, sensors and biomedicine, and analytical chemistry, it can solve the problems of variable elution, difficulty and time-consuming of protein templates.

Pending Publication Date: 2022-03-01
TIANJIN POLYTECHNIC UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Aiming at the deficiencies of the prior art, the technical problems to be solved by the present invention are the poor selectivity and sensitivity of trace protein detection, long time-consuming, easy denaturation of protein templates in the molecular imprinting process, and difficulty in elution.
[0008] The technical scheme of the present invention to solve the problems of poor selectivity and sensitivity of trace protein detection, long time-consuming, easy denaturation of protein template and difficult elution in molecular imprinting process is to provide a silver ion catalyzed protein imprinting optical and electrochemical sensor Preparation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] a) Soak the cleaned bare carbon electrode in an aqueous solution of 0.01% by mass of 3-aminopropyltriethoxysilane for treatment, and introduce amino groups on the surface of the bare carbon electrode;

[0018] b) preparing bovine serum protein mass percentage concentration of 0.01%, sodium alginate mass percentage concentration of 0.1%, acrylamide mass percentage concentration of 0.5%, ammonium persulfate mass percentage concentration of 0.01%, and carboxylated graphene mass percentage concentration of 0.01% mixture aqueous solution A, standing for defoaming and placing it in a sterile container for later use; preparing N, N-methylenebisacrylamide mass percentage concentration 0.01% and silver nitrate mass percentage concentration 0.01% mixture aqueous solution B; preparing calcium nitrate mass percentage concentration 0.1 % calcium nitrate aqueous solution;

[0019] c) Quickly mix the mixture aqueous solution A obtained in step b) with the mixture aqueous solution B, a...

Embodiment 2

[0022] a) Soak the cleaned bare carbon electrode in a 2% by mass aqueous solution of 3-aminopropyltriethoxysilane for treatment, and introduce amino groups on the surface of the bare carbon electrode;

[0023] b) preparing ovalbumin mass percentage concentration 5%, sodium alginate mass percentage concentration 5%, acrylamide mass percentage concentration 10%, ammonium persulfate mass percentage concentration 1%, carboxylated graphene mass percentage concentration 1% mixture aqueous solution A, static defoaming and placed in a sterile container for subsequent use; prepare N, N-methylenebisacrylamide mass percentage concentration 0.5% and silver nitrate mass percentage concentration 0.5% mixture aqueous solution B; prepare calcium nitrate mass percentage concentration 5 % calcium nitrate aqueous solution;

[0024]c) Quickly mix the mixture aqueous solution A obtained in step b) with the mixture aqueous solution B, and coat the mixed aqueous solution on the bare carbon electrode...

Embodiment 3

[0027] a) Soak the cleaned bare carbon electrode in an aqueous solution of 0.5% by mass of 3-aminopropyltriethoxysilane for treatment, and introduce amino groups on the surface of the bare carbon electrode;

[0028] b) Prepare a mixture aqueous solution A with a concentration of 1.5% by mass of fibronectin, 1% by mass of sodium alginate, 2% by mass of acrylamide, 0.2% by mass of ammonium persulfate, and 0.1% by mass of carboxylated graphene , standing for defoaming and placing it in a sterile container for subsequent use; prepare a mixture aqueous solution B with a mass percentage concentration of 0.1% of N,N-methylenebisacrylamide and a mass percentage concentration of silver nitrate of 0.15%; prepare calcium nitrate with a mass percentage concentration of 0.3% Calcium nitrate aqueous solution;

[0029] c) Quickly mix the mixture aqueous solution A obtained in step b) with the mixture aqueous solution B, and coat the mixed aqueous solution on the bare carbon electrode obtaine...

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Abstract

The invention provides a preparation method of a silver ion catalytic western blot optical and electrochemical sensor. The method comprises the following steps: firstly, coating a mixed aqueous solution of template protein, a functional monomer, a cross-linking agent and sodium alginate on an amino-introduced bare carbon electrode; the protein molecularly imprinted hydrogel modified electrode is prepared through a free radical polymerization reaction initiated by silver ion catalyzed ammonium persulfate. And reducing the silver ions in the hydrogel to obtain the silver nanoparticles uniformly dispersed in the hydrogel. And the detection sensitivity of the protein is greatly improved by using the silver nanoparticles as the enhanced substrate. A Raman spectrum probe and an electrode modified by protein molecular imprinting hydrogel are combined to form an optical sensor capable of rapidly detecting and identifying protein; the prepared protein molecularly imprinted hydrogel modified electrode can be simultaneously used for an optical sensor and an electrochemical sensor of surface enhanced Raman spectroscopy, so that the detection efficiency and reliability are greatly improved, and the application prospect is good.

Description

technical field [0001] The invention relates to a preparation method of a silver ion catalyzed protein imprinting optical and electrochemical sensor, which belongs to the intersecting fields of functional materials, analytical chemistry, sensors and biomedicine. [0002] The invention relates to technical fields such as hydrogel, molecular imprinting, protein detection, Raman spectroscopy and electrochemistry. Specifically relates to a preparation method and application of a silver ion catalyzed western blot optical and electrochemical sensor. Background technique [0003] With the development of artificial intelligence and higher detection requirements, various sensors have emerged, such as enzyme biosensors, cell sensors, DNA biosensors, and microbial sensors. Electrochemical biosensors are selective and specific, and are ideal analytical tools for quantitative or semi-quantitative detection of substances. They have been widely used in many fields such as environmental mo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/30G01N27/327G01N21/65
CPCG01N27/308G01N27/3278G01N21/658
Inventor 赵孔银藺塬聖杨振浩薛涛杨正春董鹏杨欣康高欣悦
Owner TIANJIN POLYTECHNIC UNIV
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