Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Detection method for inhibiting escherichia coli in-vitro transcriptional activity by bacterial transcription inhibitor

A technology of in vitro transcription and Escherichia coli, which is applied to the determination/testing of microorganisms, biochemical equipment and methods, etc., and can solve problems such as the environment and the danger of experimenters

Pending Publication Date: 2022-03-08
JILIN AGRICULTURAL UNIV
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because this method involves the use of radioactive materials, it has great potential danger to the environment and experimenters

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Detection method for inhibiting escherichia coli in-vitro transcriptional activity by bacterial transcription inhibitor
  • Detection method for inhibiting escherichia coli in-vitro transcriptional activity by bacterial transcription inhibitor
  • Detection method for inhibiting escherichia coli in-vitro transcriptional activity by bacterial transcription inhibitor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0028] In order to make the object, technical solution and advantages of the present invention more clear, the present invention will be further described in detail below in conjunction with the examples. The specific embodiments described here are only used to explain the present invention, not to limit the invention.

[0029] Firstly, the materials and main reagents used in the following implementation and the preparation of related solutions are explained:

[0030] PKK3535 plasmid (gifted by the University of California, Brosius);

[0031] E.coli D7 prrn strain (gifted by SQUIRES, Tufts University);

[0032] Main reagents: Pfu DNA polymerase and RNase Inihibitor were purchased from Biyuntian Biotechnology Co., Ltd.; E.coli RNA polymerase was made in our laboratory based on the method of Svetlov; agarose gel DNA recovery kit was purchased from Beijing Tiangen Biochemical Co., Ltd. The company; the plasmid extraction kit was purchased from Novozyme Biotechnology Co., Ltd.; ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a detection method for inhibiting escherichia coli in-vitro transcriptional activity by a bacterial transcription inhibitor. The detection method comprises the following steps: preparing an escherichia coli in-vitro transcription pKK450-DNA template; the preparation method comprises the following steps: preparing a fluorescent molecular beacon M.B. By taking a pKK3535 plasmid as a template; preparing an escherichia coli in-vitro transcription system, and detecting the in-vitro transcription inhibition activity of the escherichia coli transcription inhibitor. Compared with the prior art, the activity of the bacterial transcription inhibitor can be accurately detected; besides, high-efficiency and rapid detection instruments such as a fluorescence microplate reader and a 96-well plate are adopted, so that a large number of to-be-detected samples can be detected at high flux, and the method has important application value for screening transcriptional inhibition drugs. On the basis of the method, the action mechanism of the antibacterial substance can be analyzed so as to determine the influence of the antibacterial substance on bacterial transcription.

Description

technical field [0001] The invention relates to the technical field of inhibiting bacterial transcription, in particular to a detection method for inhibiting Escherichia coli in vitro transcription activity by a bacterial transcription inhibitor. Background technique [0002] With the increasingly serious global bacterial resistance, many bacteria have developed serious drug resistance, and a large number of marketed antibiotics have lost their antibacterial effect. The development of new antibacterial substances is imminent, and natural products such as antimicrobial peptides and secondary metabolites of symbiotic bacteria are new directions for the screening of antibacterial substances in the future. In the process of screening a large number of antibacterial substances, it is necessary to develop an economical, rapid, and high-throughput activity detection method to determine its antibacterial mechanism. Only by knowing its antibacterial mechanism can we safely, reasonabl...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/6818C12Q1/686C12Q1/689
CPCC12Q1/6818C12Q1/686C12Q1/689C12Q2600/158C12Q2600/178C12Q2600/106C12Q2525/301C12Q2563/107C12Q2565/1015C12Q2527/125C12Q2527/127C12Q2521/119
Inventor 贺承光周海蒙陈伟东王鑫闫雨欣孔令聪马红霞刘悦华李昊轩孙喆
Owner JILIN AGRICULTURAL UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com