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Induction culture medium of polygonum cuspidatum root callus and application of induction culture medium

A technology for inducing medium and callus, applied in the field of plant tissue culture, can solve the problems of low callus induction rate of Polygonum cuspidatum root, less research on callus induction of Polygonum cuspidatum root, etc. , the effect of promoting growth and reproduction

Active Publication Date: 2022-03-25
广州远想医学生物技术有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] At present, a large number of studies have reported methods for inducing callus through tissues such as leaves, petioles, and stems of Polygonum cuspidatum, but there are few studies on the induction of callus in roots of Polygonum cuspidatum, and it is also facing the problem of low callus induction rate in roots of Polygonum cuspidatum

Method used

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  • Induction culture medium of polygonum cuspidatum root callus and application of induction culture medium
  • Induction culture medium of polygonum cuspidatum root callus and application of induction culture medium
  • Induction culture medium of polygonum cuspidatum root callus and application of induction culture medium

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Embodiment 1～19

[0039] Embodiment 1~19, a kind of induction medium of Polygonum cuspidatum root callus and its preparation method

Embodiment 1

[0041] A preparation method for induction medium of root callus of Polygonum cuspidatum, comprising the following steps:

[0042] 1) 200 times mother liquor of iron salt: weigh FeSO4 ·7H 2 O, Na2-EDTA 2H 2 Dissolve O in 900ml of purified water, heat in an induction cooker, stir while heating to prevent violent boiling, after heating for 1 hour, cool to room temperature, and set the volumetric flask to 1000ml.

[0043] 2) 100 times the mother liquor of a large number of elements: Weigh potassium nitrate, magnesium sulfate, calcium chloride, ammonium chloride, potassium dihydrogen phosphate, and disodium hydrogen phosphate in sequence, and dissolve them in 900ml of water in turn, stir and dissolve with a magnetic stirrer, and when one of them After the first compound is completely dissolved, add the next compound. After all the compounds are dissolved, the volumetric flask is set to 1000ml.

[0044] 3) Preparation of 100-fold mother liquor of trace elements: Weigh about 900ml ...

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Abstract

The invention relates to the technical field of plant tissue culture, and particularly discloses an induction culture medium for polygonum cuspidatum root callus and application of the induction culture medium. The induction culture medium disclosed by the invention is prepared from the following components in concentration: 3.75 to 5.19 mg / L of major elements, 38.82 to 50.64 mg / L of trace elements, 60 to 70 mg / L of ferric salt, 101.56 to 201.94 mg / L of organic substances and 1.8 to 2.5 mg / L of hormone, the organic substances are inositol, nicotinic acid, pyridoxine hydrochloride, thiamine hydrochloride, glycine, glutamic acid and resveratrol. According to the method, the polygonum cuspidatum root meristem is used as a material, the induction culture medium is correspondingly optimized, the induction rate of the polygonum cuspidatum root callus can be remarkably improved, components of the induction culture medium cooperate with one another, growth and reproduction of the callus are promoted, and growth of the callus is facilitated.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a medium for inducing callus of Polygonum cuspidatum root and application thereof. Background technique [0002] Polygonum cuspidatum (Reynoutria japonica Houtt.), Polygonaceae, perennial herb. According to records, the rhizome of Polygonum cuspidatum has the effects of promoting blood circulation and removing blood stasis, clearing away heat and dampness, resolving phlegm and relieving cough, as well as hemostatic and anti-inflammatory effects. Studies have shown that the main active ingredients in the extract of Polygonum cuspidatum are resveratrol and emodin. Resveratrol can inhibit the activity of melanocytes, promote the activity of macrophages, and have related pharmacological effects on reducing blood lipids and inhibiting platelet activity. effect. At the same time, resveratrol also has various beneficial effects such as anti-oxidation, anti-free radicals, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/002A01H4/00
Inventor 邵静陈玉容陈如琼
Owner 广州远想医学生物技术有限公司
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