Collagen-based dura mater and preparation method thereof

A technology of collagen membrane and dura mater, applied in the field of collagen-based dura mater and its preparation, can solve the problems of discounted collagen effect and poor mechanical properties of collagen, and achieve the effects of excellent flexibility, good mechanical strength and good anti-adhesion performance

Active Publication Date: 2022-03-25
BEIJING ALLGENS MEDICAL SCI & TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the mechanical properties of collagen are poor, and it needs to act together with the carrier on the defect site, which greatly reduces the effect of collagen; in addition, due to the strong adhesion of collagen, it is often necessary to add cross-linking or anti-adhesion ingredients during use. To prevent the adhesion of collagen and brain tissue to cause complications such as epilepsy

Method used

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  • Collagen-based dura mater and preparation method thereof
  • Collagen-based dura mater and preparation method thereof
  • Collagen-based dura mater and preparation method thereof

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preparation example Construction

[0114] The present invention also provides a preparation method of the collagen-based dura mater provided above, the preparation method comprising:

[0115] (1) preparing mineralized collagen particles;

[0116] (2) Utilize type I collagen to prepare a first collagen film and a second collagen film, and utilize the mineralized collagen particles and the type I collagen to prepare a composite collagen film layer; the first collagen film and the second collagen The ratio of film thickness is 1:(1.5~2);

[0117] (3) After spraying the adhesive medium on the first collagen film and superimposing and compounding at least one layer of the second collagen film, the collagen film layer is obtained through rolling; the adhesive medium is prepared from type I collagen with a solid content of 0-0.015% collagen gel solution;

[0118] (4) Compounding the composite collagen film layer on the collagen film layer, and performing roll forming and cross-linking treatment on the compounded mater...

Embodiment 1

[0194] (1) Preparation of mineralized collagen particles:

[0195] (11) Dissolving type I collagen in hydrochloric acid, nitric acid or acetic acid to prepare a collagen acid solution with a concentration of 0.1 g / mL;

[0196] (12) Add calcium salt solution dropwise to the collagen acid solution; wherein, the amount of calcium ions added is 1 mol of calcium ions corresponding to each gram of collagen;

[0197] (13) drop phosphoric acid solution in step (12) gained solution; Wherein the mol ratio of the add-on of phosphate ion and calcium ion add-on in step (12) is Ca / P=1.5:1;

[0198] (14) NaOH solution is added dropwise to the solution obtained in step (13) to obtain a mixed solution; wherein, the pH value of the mixed solution is 6.5±0.5;

[0199] (15) After the mixed solution obtained in step (14) was left to stand for 8 hours, it was centrifuged at a speed of 4000r / min and the precipitate was taken out, and then the precipitate was air-dried at 60°C for 60 hours to obtain...

Embodiment 2

[0219] (1) Preparation of mineralized collagen particles:

[0220] (11) Dissolving type I collagen in hydrochloric acid, nitric acid or acetic acid to prepare a collagen acid solution with a concentration of 0.01g / mL;

[0221] (12) Add calcium salt solution dropwise to the collagen acid solution; wherein, the amount of calcium ions added is 0.1 mol of calcium ions corresponding to each gram of collagen;

[0222] (13) Add phosphoric acid solution dropwise in step (12) gained solution; Wherein the mol ratio of the add-on of phosphate ion and calcium ion add-on in step (12) is Ca / P=1:1;

[0223] (14) NaOH solution is added dropwise to the solution obtained in step (13) to obtain a mixed solution; wherein, the pH value of the mixed solution is 6.5±0.5;

[0224] (15) After the mixed solution obtained in step (14) was left to stand for 4 hours, it was centrifuged at a speed of 3000r / min and the precipitate was taken out, and then the precipitate was air-dried at 50°C for 72 hours t...

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Abstract

The invention provides a collagen-based dura mater and a preparation method thereof. The collagen-based dura mater comprises a collagen mater layer facing a brain tissue side and a composite collagen mater layer facing a skull side, the collagen membrane layer comprises at least two layers of collagen membranes, and the collagen membranes are formed by I-type collagen; the collagen film layer is a compact layer obtained by spraying a bonding medium on at least two layers of collagen films, overlapping and compounding, and rolling; the at least two layers of collagen membranes comprise a first collagen membrane and at least one layer of second collagen membrane, and the first collagen membrane faces the brain tissue side; the thickness ratio of the first collagen membrane to the second collagen membrane is 1: (1.5-2); the composite collagen membrane layer is formed by compounding type I collagen and mineralized collagen particles, and the interior of the composite collagen membrane layer is of a porous structure; after the collagen membrane layer and the composite collagen membrane layer are compounded, the compounded material is sequentially subjected to rolling forming and crosslinking treatment, and the collagen-based dura mater is obtained. The collagen-based dura mater provided by the invention has excellent mechanical properties and anti-adhesion performance.

Description

technical field [0001] The invention relates to the technical field of biomedical materials, in particular to a collagen-based dura mater and a preparation method thereof. Background technique [0002] The dura mater is an important barrier on the surface of the human brain tissue. It is located under the skull and covers the brain tissue and the spine. It is a thick and tough double-layer membrane. Its main function is to protect the brain, prevent cerebrospinal fluid leakage, maintain Cranial nerves are stable. Dural metastasis of malignant tumors, tumor invasion, and intraoperative resection involvement are the main causes of dural injury. At the same time, patients with dural injury are often accompanied by extensive brain swelling, resulting in dural defect, and even intracranial infection, Serious complications such as encephalocele and epilepsy, so timely and effective repair of defective dura mater tissue can avoid the above-mentioned related complications and reduc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/24A61L27/56A61L27/58
CPCA61L27/24A61L27/56A61L27/58A61L2430/34C08L89/00
Inventor 崔云宋天喜何志敏胡艳丽朱金亮吴晶晶胡刚仇志烨
Owner BEIJING ALLGENS MEDICAL SCI & TECH
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