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Strain and application thereof
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A technology of fermenting bacteria and Lactobacillus plantarum, applied in the field of microorganisms, can solve problems such as no bacterial strains
Active Publication Date: 2022-04-01
AUSNUTRIA DAIRY CHINA
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[0006] At present, the strains allowed to be used in infant food by the state mainly come from abroad, and there is no strain from Chinese breast milk
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Embodiment 1
[0054] Example 1: strain screening, identification and evaluation
[0055] 1. Preparation of experimental strains
[0056] Before activation, the strains were stored in a -20°C refrigerator with 70% glycerol. Before use, they were continuously activated once in MRS medium with a 3% inoculum, and were used for subsequent experiments after streaking and purification.
[0059] Take 1 loop of the bacterial suspension with an inoculation loop, streak it on the MRS plate, incubate at 37°C for 24 hours, and observe the characteristics of the colonies. Pick a single colony, stain with Gram, and observe the cell morphology under a biological microscope.
[0060] Colony morphology such as figure 1 As shown, the colony shape of the strain is nearly round, milky white, moist on the surface, without wrinkles, protruding in the middle, and neat at the edges. After Gram s...
Embodiment 2
[0092] Example 2: Application of Lactobacillus plantarum AUH2103 in synbiotic preparations
[0093] Lactobacillus plantarum AUH2103 was cultured on a large scale, the culture was freeze-dried, and fructo-oligosaccharides and galacto-oligosaccharides were added with a recommended volume of 2% to 6% to produce a synbiotic preparation.
[0095] Dissolve 4-7% sucrose in boiling water, wait until it is cooled to 60-70°C, add 12% skimmed milkpowder to dissolve evenly, homogenize, sterilize at 95-105°C for 10 minutes, inoculate with 2% Lactobacillus plantarum AUH2103 ( 2.5×10 9 CFU / mL), cultured at 32-37°C. A total of 2% to 6% of fructo-oligosaccharides and galacto-oligosaccharides can be added.
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technical field [0001] The invention relates to the field of microorganisms, in particular to strains and applications thereof. Background technique [0002] Numerous studies have demonstrated that breastfed infants have a lower incidence of infectious diseases compared to formula-fed infants. [0003] There are many microorganisms in breast milk, mainly including Lactobacillus and Bifidobacterium, etc., which are conducive to the formation of microbial populations in infants and enhance intestinal function. Therefore, it is of great significance to screen out lactic acidbacteria with excellent probiotic potential from breast milk. [0004] The utilization rate of oligosaccharides and prebiotics of harmful bacteria is extremely low, so oligosaccharides can selectively promote the growth and reproduction of beneficial bacteria, enhance the colonization of probiotics, inhibit the growth of spoilage bacteria in the intestinal tract, and play a healthy role on the host. The o...
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