Method for detecting various water-soluble vitamins

Abstract

The invention provides a method for detecting various water-soluble vitamins B2, B5, B6, B9 and MMA (methyl methacrylate), which comprises the following steps: (1) taking a blood sample, and directly diluting with a buffer solution to obtain a sample to be loaded; (2) carrying out anion exchange solid-phase extraction column extraction on the to-be-loaded sample to obtain a required eluent; (3) blow-drying and redissolving the eluent at 40 DEG C to obtain a sample to be detected; and (4) performing LC-MS/MS analysis on the to-be-detected sample, and detecting to obtain the content of the water-soluble vitamins. According to the method, various water-soluble vitamins can be detected at the same time, precipitation and blow-drying are not needed before solid-phase extraction, sample loading can be carried out only after simple dilution, compared with complex methods such as precipitation and blow-drying, the detection efficiency is improved, the pretreatment time is saved, sample loss caused by protein precipitation is avoided, and the requirement for accurate and rapid clinical detection is met.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a detection method for water-soluble vitamins. Background technique [0002] Water-soluble vitamins are a type of trace organic substances that humans and animals must obtain from food in order to maintain normal physiological functions, and play an important role in human growth, metabolism, and development. Long-term deficiency of water-soluble vitamins can easily lead to diseases caused by some physiological dysfunctions, such as pernicious anemia, easy inflammation, and allergies in adults; slow growth in young children, affecting normal development; lack of folic acid in pregnant women can cause pre-eclampsia and placental separation. rate increased. Therefore, establishing an accurate method to simultaneously monitor water-soluble vitamins and their active forms in the body is helpful for timely detection of human health risks caused by vitamin deficiency or excess, as well as a...

AI Technical Summary

Problems solved by technology

This method has low sensitivity for some analytes and cannot meet the requirements for the lower limit of quantitation LLOQ. For example, the LLOQ in the VB5 pantothenic acid patent is 1ng / mL, and the sample concentration of the chromatogram in the accompanying drawing is 130.5ng / mL (Fig. 7), and the response is 9000cps. According to this response conversion LLOQ response is less than 70cps; similarly, the LLOQ response of VB6 is less than 150cps (other image 3 ), does not meet the detection requirements of the lower limit of quantification

Guangdong Nanxin Medical Technology Co., Ltd. applied for Chinese patent 202010074354.6 on April 1, 2020, "A method and application for simultaneously detecting multiple water-soluble vitamins in blood samples", although the patent can detect vitamins in 10 minutes with one injection There are 13 kinds of water-soluble vitamins, but the relevant values ​​of the precision and accuracy of the sample detection are not clearly given in the instructions. Just looking at the peak shape and response of a sample in the attached picture, it is impossible to conclude that the analyte is within this range The conclusion of a good linear relationship; and for a method to detect multiple substances at the same time, the quality of some analyte chromatograms is poor, and accurate detection results and good reproducibility cannot be obtained

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