Application of photosensitized oxidized zingiberaceae plant extract in antibacterial and antitumor drugs

A technology of plant extracts and anti-tumor drugs, applied in the directions of anti-tumor drugs, anti-bacterial drugs, plant/algae/fungus/moss components, etc., to achieve the effect of enhanced antibacterial and anti-tumor activity

Pending Publication Date: 2022-04-08
YUNNAN UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are relatively few studies on the antitumor activity of ginger volatile oil.

Method used

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  • Application of photosensitized oxidized zingiberaceae plant extract in antibacterial and antitumor drugs
  • Application of photosensitized oxidized zingiberaceae plant extract in antibacterial and antitumor drugs
  • Application of photosensitized oxidized zingiberaceae plant extract in antibacterial and antitumor drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The extraction method of ginger volatile oil comprises the following steps:

[0025] S1: Weigh an appropriate amount of ginger medicinal material and divide it into two equal parts, place them in two 1000ml round bottom flasks respectively, add deionized water to submerge the medicinal material, and soak for 0.5h;

[0026] S2: Connect the volatile oil extractor and the reflux condenser, put it in a temperature-adjusted oil bath and heat it slowly until it boils, control the temperature of the oil bath, keep it slightly boiling for 5 hours, stop heating, and let it stand for 1 hour;

[0027] S3: Collect the extracted volatile oil, divide it into two equal parts, seal them, number them respectively, and store them in a refrigerated environment at 4°C for later use.

[0028] The photooxidative reaction of ginger volatile oil includes the following steps:

[0029] S1: Dissolve the extracted Zingiberaceae plant extract in an organic solvent, add curcumin, and mix well;

[...

Embodiment 2

[0032] TLC detection and analysis of chemical composition changes before and after ginger volatile oil photooxidation;

[0033] Take 2 mg each of the unphotosensitive oxidized ginger volatile oil (sample 1) and the photosensitive oxidized ginger volatile oil (sample 2) extracted in Example 1, dissolve them in 0.5 mL acetone, and absorb 5 μL of each of the above solutions with a capillary tube. After spotting on the board, develop with petroleum ether: ethyl acetate (5:1), and develop color with peroxide developer;

[0034] The volatile oil of ginger without light and the volatile oil of ginger added with curcumin and light were detected by thin layer chromatography. The results showed that no peroxides could be detected in ginger volatile oil without photooxidation (sample 1), while multiple peroxides could be detected in ginger volatile oil after photooxidation (sample 2), such as figure 1 shown. It can be seen that curcumin catalyzes the photochemical reaction of the chemi...

Embodiment 3

[0036] Determination of antibacterial activity of ginger volatile oil;

[0037] 1) Determination of the inhibition zone of ginger volatile oil

[0038] Gram-positive bacteria and negative bacteria were inoculated into liquid LB medium, and cultured in the dark at 37°C, 200r·min-1 for 12h; Candida albicans and its drug-resistant bacteria were inoculated into liquid Sabouraud medium, 28°C, 200r·min -1 dark culture for 24h. Dilute each bacterial solution to 1×106 ~ 1×107 cfu·mL-1 with liquid culture medium for later use. The antibacterial activity of ginger volatile oil without photosensitization oxidation and photosensitization oxidation was tested by antibacterial disc diffusion method.

[0039] Spread the diluted indicator bacteria solution evenly on the solid medium, weigh 90 mg each of ginger volatile oil without photooxidation (sample 1) and ginger volatile oil after photooxidation (sample 2), add 900 μL acetone to dissolve to With a mass concentration of 100 μg·μL-1, ta...

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Abstract

The invention discloses an application of a photosensitized and oxidized zingiberaceae plant extract in antibacterial and antitumor drugs, which comprises the following steps: mixing an extract of zingiberaceae plants such as ginger or alpinia japonica fruits with a photosensitizer such as curcumin, and performing photosensitized oxidation under an illumination condition; the change of chemical components of the zingiberaceae plant extract subjected to photo-catalytic oxidation is detected by thin-layer chromatography after photo-oxidation, the change of antibacterial activity is determined by an antibacterial paper diffusion method and a double dilution method, and the change of anti-tumor activity is determined by an MTT (Methyl Thiazolyl Tetrazolium) method. Results show that the fresh ginger volatile oil subjected to photosensitized oxidation generates new chemical components, the antibacterial activity is enhanced, the antibacterial spectrum is widened, the anti-tumor cell proliferation effect is enhanced, and certain dose dependence is shown. A research result shows that after the zingiberaceae plant extract is subjected to photosensitized oxidation, a product with higher antibacterial and anti-tumor activity is generated. Research results provide a new way for research and development of antibacterial and antitumor natural drugs.

Description

technical field [0001] The invention belongs to the technical field of antibacterial and anticancer drugs, and in particular relates to the application of photosensitive oxidized ginger plant extracts in antibacterial and antitumor drugs. Background technique [0002] Ginger is a plant of Zingiberaceae Zingiberaceae. It is a traditional food and medicine homologous plant. It has the effects of relieving the exterior and dispelling cold, warming the middle and relieving cough, resolving phlegm and relieving cough, and detoxifying fish and crabs. Ginger volatile oil is an important chemical component in ginger. In recent years, it has been proved to have pharmacological effects such as antibacterial, anticancer, and antioxidative. Studies have shown that ginger volatile oil has inhibitory effects on Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Candida albicans, Penicillium and other bacteria, mainly by destroying cell membranes and inhibiting biofilms to ac...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/9068A61K36/9062A61K41/00A61P35/00A61P31/04
Inventor 赵庆张晓梅黎晓菊黄之镨韩峻司禹陈蕾庹呈杰张冰张科涛许小蓉刘朝金李思恒许玉潇
Owner YUNNAN UNIV OF TRADITIONAL CHINESE MEDICINE
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