Biologically modified oxidized regenerated cellulose hemostatic anti-adhesion material and preparation method thereof
A technology of regenerated cellulose and biological modification, applied in the field of biomedical materials and their preparation, can solve problems such as incompatibility with protein materials and low biocompatibility.
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Embodiment 1
[0044] Step 1 (S1): Preparation of bacterial cellulose nanoslurry. Specifically, bacterial cellulose wet film (provided by Guilin Qihong Technology Co., Ltd.) was directly used, which was mechanically pulverized and acidified with 70% sulfuric acid in sequence. The volume ratio of slurry to sulfuric acid was 1:10, and the acidification time was 6 hours. The concentration of the nanocellulose slurry obtained after washing, centrifugal separation and dialysis is about 1.2g / mL, the radial dimension of the nanofiber is 200-400nm, and the transverse dimension is 20-50nm.
[0045] Step 2 (S2): Oxidation of regenerated cellulose. Specifically, the regenerated cellulose in S1 was carboxylated and formylated to increase its water solubility and reactivity. The carboxylation reaction was carried out in a TEMPO-NaBr-NaClO selective oxidation system (provided by Shanghai Macklin Company, the mass ratio of regenerated cellulose to oxidizing reagent was 10:1), mainly to oxidize C6 primary ...
Embodiment 2
[0051] Step 1 (S1): Preparation of bacterial cellulose nanoslurry. Specifically, bacterial cellulose wet film (provided by Guilin Qihong Technology Co., Ltd.) was directly used, which was mechanically pulverized and acidified with 65% sulfuric acid in sequence. The volume ratio of slurry to sulfuric acid was 1:8, and the acidification time was 6 hours. The concentration of the nanocellulose slurry obtained after water washing, centrifugal separation and dialysis is about 1.4g / mL, the radial dimension of the nanofiber is 1000-2000nm, and the transverse dimension is 50-100nm.
[0052] Step 2 (S2): Oxidation of regenerated cellulose. Specifically, the regenerated cellulose in S1 was carboxylated and formylated to increase its water solubility and reactivity. The carboxylation reaction was carried out in a TEMPO-NaBr-NaClO selective oxidation system (provided by Shanghai Macklin Company, the mass ratio of regenerated cellulose to oxidizing reagent was 10:1), mainly to oxidize C6 ...
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