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Method for accurately identifying plant protein interaction

A plant protein, precise technology, applied in the field of molecular genetics, can solve the problems of cumbersome experimental process, and achieve the effect of high throughput, high efficiency and reliable technical means, and strong accuracy

Pending Publication Date: 2022-04-08
BEIJING FORESTRY UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The method provided by the invention overcomes the false positive problem caused by high-throughput sequencing technology and the cumbersome experimental process of repeatedly using the yeast two-hybrid system to verify the interaction relationship, and is more efficient, accurate and simple

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  • Method for accurately identifying plant protein interaction
  • Method for accurately identifying plant protein interaction
  • Method for accurately identifying plant protein interaction

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Experimental program
Comparison scheme
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Embodiment 1

[0052] A method for accurately identifying plant protein interactions (flowchart see figure 1 ), consisting of the following steps:

[0053] The selected material is poplar vascular tissue, and the target gene of protein kinase Potri.003G049700 (Photoregulatory Protein Kinase 4, PPK4) is identified;

[0054] Step S1, using 15 groups of poplar vascular tissue transcriptome sequencing (Table 1) in the public database (NCBI), to obtain genome-wide expression profile data.

[0055] Table 1 Transcriptome sequencing data of 15 groups of poplar vascular tissues

[0056]

[0057] In step S2, based on the expression profile data obtained in step S1, a co-expression regulation network of poplar vascular tissue is constructed, and target genes having the same expression pattern as PPK4 are identified, and the specific steps are as follows:

[0058] Firstly, WGCNA was used to construct the co-expression regulatory network of the poplar woody part. The gene screening criteria for cons...

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Abstract

The invention relates to the technical field of molecular genetics, in particular to a method for accurately identifying plant protein interaction. According to the invention, co-expression regulatory network analysis and a yeast two-hybrid technology are combined, so that candidate target genes with significant correlation expression level in the same tissue as candidate genes can be obtained with high throughput, and the interaction relationship between the candidate genes and the candidate target genes can be verified by using the yeast two-hybrid technology; the method overcomes the problem of false positive caused by a high-throughput sequencing technology and the problem that the interaction process is time-consuming and tedious by repeatedly utilizing a yeast double-hybrid technology, is high in throughput and high in accuracy, and provides a rapid, efficient and reliable technical means for identifying a plant protein interaction mechanism.

Description

technical field [0001] The invention relates to the technical field of molecular genetics, in particular to a method for accurately identifying plant protein interactions. Background technique [0002] Protein-protein interaction refers to the process in which two or more protein molecules form a protein complex through non-covalent bonds. The interaction between proteins constitutes a major component of the cell biochemical reaction network and participates in the Important biological processes such as signal transduction. The current methods for identifying protein interactions include constructing a co-expression regulatory network, yeast two-hybrid, bimolecular fluorescence complementation, etc. [0003] Gene co-expression network analysis is based on the dynamic changes of gene expression signal values, calculates the co-expression correlation between genes, and establishes a gene transcription regulation model, so as to find one or more species at different developmen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G16B15/30G16B20/00G16B25/10G16B40/30G16B50/30
Inventor 张德强王丹房媛媛
Owner BEIJING FORESTRY UNIVERSITY