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Thiophosphoric acid blood purification modified membrane and preparation method thereof

A blood modification technology of phosphorothioate, which is applied in the field of medicine, can solve the problems that have not been reported about Amifostine directly modifying blood purification membranes, and achieve the effects of improving blood compatibility, reducing risks, and promoting the growth of hematopoietic cells

Active Publication Date: 2022-04-12
XIANGYA HOSPITAL CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, there is no relevant report on the direct modification of blood purification membrane with Amifostine

Method used

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  • Thiophosphoric acid blood purification modified membrane and preparation method thereof
  • Thiophosphoric acid blood purification modified membrane and preparation method thereof
  • Thiophosphoric acid blood purification modified membrane and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0029] A preparation method of phosphorothioate blood purification modified membrane, comprising the following steps:

[0030] (1) Preparation of O-carboxymethyl chitosan

[0031] Add 10 g of chitosan to 50 mL of 65% NaOH solution for alkalization for 2.5 h, stir evenly and cool to room temperature; add 30 g of chloroacetic acid to react for 2 h, adjust the pH to 7.0, wash with ethanol, filter, separate and purify, and vacuum dry at 50 °C. O-carboxymethyl chitosan is obtained; the preparation reaction equation of O-carboxymethyl chitosan is shown in formula II;

[0032]

[0033] (2) Preparation of Amifostine Modified Chitosan (AMCTS, Amifostine Modified chitosan)

[0034] Dissolve 10g O-carboxymethyl chitosan in 50ml N,N-dimethylformamide and stir to form a homogeneous solution, add carbodiimide and N-hydroxysuccinimide, activate at 4°C for 15min, Add Amifostine to the reaction system and continue the reaction for 3 hours; wash and filter with ethanol, separate and purify...

Embodiment 2

[0039] A preparation method of phosphorothioate blood purification modified membrane, comprising the following contents:

[0040] (1) Preparation of O-carboxymethyl chitosan

[0041] Add 10 g of chitosan to 30 mL of 50% NaOH solution for alkalization for 10 h, stir evenly and cool to room temperature; then add 20 g of chloroacetic acid to react for 2 h, finally adjust the pH to 7.0, wash with ethanol, filter, separate and purify, and vacuum dry at 50 °C , to obtain O-carboxymethyl chitosan;

[0042] (2) Preparation of Amifostine Modified Chitosan (AMCTS, Amifostine Modified chitosan)

[0043] Dissolve 10g O-carboxymethyl chitosan in 80ml N,N-dimethylformamide and stir to form a homogeneous solution, add carbodiimide and N-hydroxysuccinimide, activate at 4°C for 15min, After adding Amifostine to the reaction system, the reaction was continued for 6 h; washed with ethanol, filtered, separated and purified, and vacuum-dried at 50 °C to obtain AMCTS; wherein the mass ratio of O-...

Embodiment 3

[0047] A preparation method of phosphorothioate blood purification modified membrane, comprising the following contents:

[0048] (1) Preparation of O-carboxymethyl chitosan

[0049] Add 10 g of chitosan to 40 mL of 60% NaOH solution for alkalization for 8 h, stir evenly and cool to room temperature. Subsequently, 30 g of chloroacetic acid was added to react for 2 hours, and finally the pH was adjusted to 7.0, washed with ethanol, filtered, separated and purified, and vacuum-dried at 50 °C to obtain O-carboxymethyl chitosan;

[0050] (2) Preparation of Amifostine Modified Chitosan (AMCTS, Amifostine Modified chitosan)

[0051] Dissolve 10g O-carboxymethyl chitosan in 50ml N,N-dimethylformamide and stir to form a homogeneous solution, add carbodiimide and N-hydroxysuccinimide, activate at 4°C for 45min, Amifostine was added to the reaction system and the reaction was continued for 4 hours; washed with ethanol, filtered, separated and purified, and vacuum-dried at 50 °C to obtai...

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Abstract

The invention discloses a thiophosphoric acid blood purification modified membrane and a preparation method thereof, and belongs to the technical field of medicines. The preparation method comprises the following steps: (1) preparation of O-carboxymethyl chitosan: alkalifying chitosan, and reacting with chloroacetic acid to obtain O-carboxymethyl chitosan; (2) preparation of modified chitosan: after carbodiimide and N-hydroxysuccinimide are added into an O-carboxymethyl chitosan solution for activation, Amifosine is added into the O-carboxymethyl chitosan solution for a reaction, and AMCTS is obtained; and (3) preparation of an AMCTS modified membrane: dissolving AMCTS and a polymer in N, N-dimethylacetamide to prepare a solution, and preparing to obtain the thiophosphoric acid blood purification modified membrane. The Amifosine is used for directly modifying the blood purification membrane, and the effects of improving the blood compatibility of the membrane, stabilizing the blood cell membrane, promoting the growth of hematopoietic cells and reducing the risk of reduction of blood cells are achieved by improving toxin removal of the membrane material and improving microinflammation and oxidation reaction.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a phosphorothioate blood purification modified membrane and a preparation method thereof. Background technique [0002] Maintenance blood purification therapy is widely used in patients with severe chronic renal insufficiency. Although the above treatments can replace part of the kidney function and promote the excretion of toxic substances from the body, some patients are prone to complications of peripheral blood pancytopenia after long-term dialysis. Fan and Nakanishi et al believe that the main reasons for the decrease of peripheral blood cells in maintenance hemodialysis patients are the level of toxins in the body and the micro-inflammatory state of the body. In the stem cell pool or dividing pool, and can be destroyed in situ in the bone marrow or shortly after the release of human blood, ineffective hematopoiesis occurs. Patients with cytopenias will develo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01D61/24B01D67/00B01D71/06B01D71/08B01D71/60B01D71/68A61M1/16
Inventor 府晓
Owner XIANGYA HOSPITAL CENT SOUTH UNIV
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