Aspergillus oryzae fermentation solid material, aspergillus oryzae strain culture medium and preparation method and application thereof
A fermentation medium, Aspergillus oryzae technology, applied in the field of Aspergillus oryzae fermentation solid material, to achieve stable quality, avoid waste of resources and environmental pollution
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[0043] As a third aspect of the present invention , the present invention provides a preparation method of soy sauce, said preparation method comprising the step of preparing fermented seeds and the step of preparing soy sauce with said fermented seeds;
[0044] The step of preparing the fermented seeds comprises: inoculating the Aspergillus oryzae species into the fermentation medium of the Aspergillus oryzae species and cultivating to prepare the fermented seeds.
[0045] In one example, the culture conditions include adopting the following gradient culture method: first culture at 26°C-34°C for 23h-27h, then at 35°C-37°C for 8h-12h, and then at 25°C-32°C for 33h -37h.
[0046] As far as the culture gradient of the present invention is “cultivated at 26°C-34°C for 23h-27h”, the temperature of the culture gradient can be selected from, including but not limited to, the following values or ranges between: 26°C, 26.5°C, 27°C , 27.5°C, 28°C, 28.5°C, 29°C, 29.5°C, 30°C, 30.5...
Embodiment 1
[0052] 1. Raw material pretreatment
[0053] (1) Bran pretreatment: the fineness of the bran is controlled at 20 mesh sieves and the proportion of the under-sieve is 30% (by weight).
[0054] (2) Pretreatment of crop straw: mixed crop straw powder (m 稻草秸秆粉 :m 小麦秸秆粉 =1:1) The fineness is controlled at 40% (weight ratio) under the 20 mesh sieve.
[0055] (3) corn flour pretreatment: the fineness of corn flour is controlled at 25 mesh sieve ratio is 65% (weight ratio).
[0056] 2. Cultivation of Aspergillus oryzae strains for soy sauce fermentation
[0057] (1) Preparation of culture medium for Aspergillus oryzae
[0058] The pretreated bran and mixed crop straw powder (m 稻草秸秆粉 :m 小麦秸秆粉 =1:1), soybean protein, bean dregs, and corn flour are respectively 45%, 20%, 4%, 16%, and 15% according to the weight ratio. After weighing and mixing the dry material, the dry material is obtained according to the weight ratio: Water=1:1.2 ratio, stir evenly, dispense into Erlenmeyer flas...
Embodiment 2
[0062] 1. Raw material pretreatment:
[0063] (1) Bran pretreatment: the fineness of the bran is controlled at 20 mesh sieves and the ratio of the under-sieve is 40% (by weight).
[0064] (2) Pretreatment of crop straw: mixed crop straw powder (m 稻草秸秆粉 :m 小麦秸秆粉 =1:1) The fineness is controlled at 30% (weight ratio) of 20-mesh sieve.
[0065] (3) corn flour pretreatment: the fineness of corn flour is controlled at 25 mesh sieves and the proportion is 85% (weight ratio).
[0066] 2. Cultivation of Aspergillus oryzae strains for soy sauce fermentation
[0067] (1) Preparation of culture medium for Aspergillus oryzae
[0068] Mix the pretreated bran and crop straw powder (m 稻草秸秆粉 :m 小麦秸秆粉 =1:1), soybean protein, bean dregs, and corn flour are respectively 35%, 22%, 6%, 19%, and 18% according to the weight ratio. After the dry material is weighed and mixed, the dry material is obtained according to the weight ratio: Water=1:1.2 ratio, stir evenly, dispense into Erlenmeyer fl...
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