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Analysis method for drugs in tissues

An analysis method and tissue technology, applied in the field of medicine, can solve the problems that drugs are easy to contaminate skin tissue, cannot judge whether there are contaminated parts and the degree of contamination in the test results, and cannot accurately locate, so as to avoid falsely high concentration of drugs in samples and avoid The effect of falsely high drug concentration and avoiding data distortion

Pending Publication Date: 2022-04-12
南京美新诺医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] It is difficult to separate the layers of skin tissue after conventional skin application and administration. It is necessary to slice all the skin tissue longitudinally and then slice horizontally to select the required part (such as a specific dermis layer), which is time-consuming, and sampling cannot accurately locate the required cortex.
After the skin is smeared and administered, the drug is likely to contaminate the skin tissue on the longitudinal section when the longitudinal section is made. At this time, the horizontal section is used to select a part of the homogenate for drug testing, and it is impossible to judge whether the test results have contaminated parts and the degree of contamination, resulting The data result is inaccurate

Method used

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  • Analysis method for drugs in tissues
  • Analysis method for drugs in tissues

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Embodiment 1

[0042] 1 Administration, sampling: using radioactively labeled compounds ( 14 C-Compound) was applied to pigs for skin administration, and the dry skin tissue was excised with a 7mm diameter skin picker at a depth of 7-8mm according to the specified time points. After taking out the skin, put it into a Petri dish, and immediately freeze it in dry ice to set the shape.

[0043] 2 Sample embedding: Pour 2.5% sodium carboxymethylcellulose (w / v) into a rectangular frame, covering only the bottom plate, and put it into liquid nitrogen to pre-freeze until it is semi-solidified. Take the skin with tweezers with the longitudinal section facing up, place vertically in semi-solidified 2.5% sodium carboxymethylcellulose (w / v), add enough 2.5% sodium carboxymethylcellulose (w / v) to cover Fill the frame with the skin, freeze in liquid nitrogen until completely frozen, and then transfer to a -20°C freezer overnight.

[0044] 3 Standard curve preparation: use rat plasma to 14C-glucose sto...

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Abstract

The invention provides a method for analyzing drugs in tissues. Quantitative analysis and detection are carried out on drug distribution conditions in in-vitro tissues by adopting a quantitative whole-body radioautography (QWBA) technology and a tissue embedding section. According to the invention, transverse slicing after longitudinal slicing is avoided, the experiment time is saved, the structure of each layer of the skin is accurately positioned, and the distribution condition of drugs in the cuticle, epidermal layer, dermis layer and subcutaneous tissue of the skin can be visually observed. And similar effects are achieved on other tissues.

Description

technical field [0001] The invention belongs to the field of medicine, in particular to a method for analyzing medicine in tissues. Background technique [0002] Transdermal absorption refers to a dosage form in which the drug is released from a specially designed device, absorbed through the intact skin, and enters the blood system of the whole body. It is usually called a transdermal drug delivery system or a transdermal therapeutic system. The main features are: [0003] ① Avoid the inactivation of drugs in the gastrointestinal tract and the first-pass effect of the liver; [0004] ② Make the blood drug concentration stable and keep it within the effective concentration range for a long time; [0005] ③Reduce the irritation of the drug to the gastrointestinal tract; [0006] ④ To improve safety, medication can be stopped at any time. [0007] Structure of the skin: The skin is composed of epidermis, dermis and subcutaneous fat tissue. The dermis and subcutaneous tiss...

Claims

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Application Information

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IPC IPC(8): G01N1/36G01N1/42G01N23/00G01T1/204
Inventor 李林雷苇张玲玲
Owner 南京美新诺医药科技有限公司