Scorpion venom derived peptide and application thereof in preparation of antibacterial or anti-inflammatory drugs
A technology of antibacterial drugs and anti-inflammatory drugs, applied in the field of scorpion venom-derived peptides and their application in the preparation of anti-bacterial or anti-inflammatory drugs, can solve the serious drug resistance of Escherichia coli, the effect of immune prevention is not ideal, antibiotics Reduced curative effect and other problems, to achieve the effect of less drug resistance, good safety, and fast sterilization speed
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[0045] Example 1
[0046] This example provides a scorpion derived peptide (named FFG-18), where the amino acid sequence is: ffgrlfklavkiipsLFK (SEQ ID NO: 1), which contains 18 amino acid residues, with an average molecular weight of 2124.65g. / mol, hydrophobic coefficient 1.03, theoretical equidistant PH is 11.73.
[0047] The scorpion derived peptide can be applied to preparing antibacterial products or anti-inflammatory drugs.
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[0048] Example 2: Synthesis Method of Scorpio Derivent Peptide FFG-18
[0049] Synthesis of Example 1 in Example 1 was synthesized by standard FMOC solid phase synthesis method, including steps: weighing 0.1 mmol resin in reactor, adding DCM swelling and activating, 1.5 ml / 5 min, wash 2 times; DMF 1.5ml / 10min, wash 2 times. Plus 25% piperidine / DMF 2 ml / 5 min; plus 25% piperidine / DMF 2 ml / 25min; DMF 1 ml / 2min, washed 6 times. To set up a resin: amino acid: TBTU: HOBT: DIEA = 1: 3: 3: 3: 6, the amount of DMF was added as a solvent, and the reaction was 3 h. 2% ninluolone solution: 0.3 g of niolinone, dissolved with 15 ml of anhydrous ethanol; with a pipette to draw more resin in a glass tube, use DMF, MeOH to wash the resin, add a ninluolone solution 200 μL, after heating 3-5 min, the resin color change was observed, and the resin did not change blue, the reaction was completely, and the reaction liquid was squeezed, and the resin in the reactor was used for DMF, MeOH ...
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[0051] Example 3: Determination of strains culture conditions and minimum antibacterial concentration
[0052] Copper green psessasis, Escherichia coli and its clinical isolates were cultured under needle, using MH medium to cultured under an oxygen, and the growth temperature of the strain was cultured under an oxygen condition. 37 ° C, using the Mai's turbidity to determine the total number of bacteria. The particles were diluted with the strain to be diluted to the specified concentration range to formulate the 96-hole drug sensitive plate. Take the number of growth bacterial suspensions, determine the concentration of the bacterial liquid and dilute to 1 × 10 5 CFU / mL, 100 μl of bacterial suspension was added to each well, and a multi-group drug concentration was provided, and the incubation of the incubation was continued in the incubator, and the results were read in 17-20 hours, and the minimum observation of the signs of bacterial growth was MIC value. Each group of test...
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