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Scorpion venom derived peptide and application thereof in preparation of antibacterial or anti-inflammatory drugs

A technology of antibacterial drugs and anti-inflammatory drugs, applied in the field of scorpion venom-derived peptides and their application in the preparation of anti-bacterial or anti-inflammatory drugs, can solve the serious drug resistance of Escherichia coli, the effect of immune prevention is not ideal, antibiotics Reduced curative effect and other problems, to achieve the effect of less drug resistance, good safety, and fast sterilization speed

Pending Publication Date: 2022-04-15
ZHUHAI PEOPLES HOSPITAL GUANGDONG PROVINCE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

And because there are many serotypes of Escherichia coli, the effect of immune prevention is not ideal, so antibacterial drugs are still the main treatment option at present
However, due to the overuse of broad-spectrum antibiotics, the degree of drug resistance of Escherichia coli has become more and more serious, and drug-resistant strains have the ability to accumulate resistance genes through horizontal gene transfer, resulting in a decrease in the efficacy of clinically available antibiotics or even complete failure

Method used

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  • Scorpion venom derived peptide and application thereof in preparation of antibacterial or anti-inflammatory drugs
  • Scorpion venom derived peptide and application thereof in preparation of antibacterial or anti-inflammatory drugs
  • Scorpion venom derived peptide and application thereof in preparation of antibacterial or anti-inflammatory drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] This example provides a scorpion venom-derived peptide (named FFG-18), its amino acid sequence is: FFGRLFKLAVKIIPSLFK (SEQ ID NO: 1), the scorpion venom-derived peptide polypeptide contains 18 amino acid residues, and its average molecular weight is 2124.65g / mol, the hydrophobic coefficient is 1.03, and the theoretical isoelectric point pH is 11.73.

[0047] The scorpion venom-derived peptide can be applied to the preparation of antibacterial products or anti-inflammatory drugs.

Embodiment 2

[0048] Example 2: Synthetic method of scorpion venom-derived peptide FFG-18

[0049] The standard Fmoc solid-phase synthesis method was used to synthesize the scorpion venom-derived peptide FFG-18 in Example 1. The steps were as follows: weigh 0.1 mmol of the resin and place it in a reactor, add DCM for swelling and activation, 1.5 mL / 5 min, and wash twice; DMF 1.5mL / 10min, wash 2 times. Add 25% piperidine / DMF 2mL / 5min; add 25% piperidine / DMF 2mL / 25min; DMF 1mL / 2min, wash 6 times. Set resin: amino acid: TBTU: HOBt: DIEA = 1:3:3:3:6, add an appropriate amount of DMF as a solvent, and react for 3 hours. Preparation of 2% ninhydrin solution: Weigh 0.3g ninhydrin and dissolve it in 15mL of absolute ethanol; pipette a dozen resins into a glass tube, wash the resin with DMF and MeOH, add ninhydrin solution for about 200 μL, heated for 3-5 minutes, observe the color change of the resin, if the resin does not turn blue, the reaction is complete, squeeze out the reaction liquid, and ...

Embodiment 3

[0051] Embodiment 3: Bacterial strain culture condition and minimum inhibitory concentration determination

[0052] Use LB medium to culture Pseudomonas aeruginosa, Escherichia coli and its clinical isolates under aerobic conditions, use MH medium to culture Staphylococcus aureus under aerobic conditions, and the growth temperature of the strains is At 37°C, the total number of colonies was determined using the McFarland method. Use the medium corresponding to the strain to dilute the test compound to the specified concentration range to prepare a 96-well drug-sensitive plate. Take the bacterial suspension in the logarithmic growth phase, measure the concentration of the bacterial solution and dilute to 1×10 5 CFU / mL, add 100 μL of bacterial suspension to each well, set multiple groups of drug concentrations, continue to incubate in the incubator, read the results after 17-20 hours, take the lowest observed drug concentration without signs of bacterial growth as the MIC value...

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Abstract

The invention belongs to the technical field of polypeptide drugs, and discloses a scorpion venom derived peptide and application thereof in preparation of antibacterial or anti-inflammatory drugs. The amino acid sequence of the scorpion venom derived peptide is as follows: FFGRLFKLAVKIIPSLFK. The scorpion venom derived peptide has low toxicity to normal cells, and has good safety; the scorpion venom derived peptide has a remarkable inhibition effect on bacteria such as escherichia coli, staphylococcus aureus and pseudomonas aeruginosa; meanwhile, expression of downstream inflammatory factors can be affected by regulation and control on NF-kappa B, and anti-inflammatory and immune regulation effects are achieved.

Description

technical field [0001] The invention belongs to the technical field of polypeptide drugs, and in particular relates to a peptide derived from scorpion venom and its application in the preparation of antibacterial or anti-inflammatory drugs. Background technique [0002] Escherichia coli (E.coli) is a Gram-negative short bacillus that can cause urinary tract, blood, skin and soft tissue infections, and severe infections may be life-threatening. And because there are many serotypes of Escherichia coli, the effect of immune prevention is not ideal, so antibiotics are still the main treatment option at present. However, due to the overuse of broad-spectrum antibiotics, the degree of drug resistance of Escherichia coli has become more and more serious, and drug-resistant strains have the ability to accumulate resistance genes through horizontal gene transfer, resulting in a decrease in the efficacy of clinically available antibiotics or even complete failure . Therefore, it is ...

Claims

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Application Information

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IPC IPC(8): C07K7/08A61K38/10A61P31/04A61P29/00A61P37/02
CPCY02A50/30
Inventor 陆骊工占美晓杨光徐清波倪文君黄文张志人李小彬胡佳丽
Owner ZHUHAI PEOPLES HOSPITAL GUANGDONG PROVINCE
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