Composite bionic body surface tissue and integrated construction method thereof

A construction method and body surface technology, applied in tissue regeneration, prosthesis, medical science, etc., can solve the problems of atrophy, blurred three-dimensional structure of the reconstructed auricle, deformation, etc., to improve the mechanical strength and meet the needs of clinical applications.

Active Publication Date: 2022-04-19
PLASTIC SURGERY HOSPITAL CHINESE ACAD OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, long-term follow-up found that the three-dimensional structure of the reconstructed auricle gradually blurred, atrophied, deformed and collapsed, and the long-term clini

Method used

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  • Composite bionic body surface tissue and integrated construction method thereof
  • Composite bionic body surface tissue and integrated construction method thereof
  • Composite bionic body surface tissue and integrated construction method thereof

Examples

Experimental program
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Example Embodiment

[0052] Example 1

[0053] as Figures 1 through 12 As shown, the present embodiment provides an integrated construction method of composite bionic surface organization, comprising the following specific steps:

[0054] S1: Add an appropriate amount of adjuvant to the cartilage decellularized matrix photosensitive gel and mix well, and obtain a mass percentage of the adjuvant with a mass of 10-50% of the cartilage bioink;

[0055] S2: Add an appropriate amount of adjuvant two to the cartilage decellularized matrix photosensitive gel and mix well to obtain a cartilage bioink with a mass percentage of 10-50% of the auxiliary agent;

[0056] S3: The appropriate amount of seed cells is added to the cartilage membrane bioink obtained by S1 to form seed cell concentrations of (1 to 50) ×10 6 A mixed solution of / mL, while adding an appropriate amount of seed cells to the cartilage bioinkant obtained by S2, forms a seed cell concentration of (1 to 50) ×10 6 Mixed solution ii of / mL;

[005...

Example Embodiment

[0059] Example 2

[0060] On the basis of Example 1, in the present embodiment, the S1 is further included S0: pretreatment of cartilage tissue to obtain a cartilage decellularized matrix photosensitive gel. The preparation process is simple, providing a biomimetic raw material cartilage decellular matrix photosensitive gel for cartilage membrane and cartilage bioink

Example Embodiment

[0061] Example 3

[0062] Based on Example 2, in the present embodiment, the cartilage tissue in the S0 is one or more of the ear cartilage, joint cartilage, rib cartilage, scapular cartilage and meniscus. It is easy to obtain and is conducive to the preparation of biomimetic reconstructed surface tissue.

[0063] It should be noted that the above cartilage tissue is usually made of pig cartilage tissue, which can be purchased in the market and can achieve industrial production.

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Abstract

The invention relates to a composite bionic body surface tissue and an integrated construction method thereof, and the method comprises the following specific steps: adding a proper amount of an auxiliary agent into cartilage acellular matrix photosensitive gel, and uniformly mixing to obtain cartilage membrane bio-ink; adding a proper amount of auxiliary agent II into the cartilage acellular matrix photosensitive gel, and uniformly mixing to obtain cartilage biological ink; adding seed cells into cartilage membrane bio-ink to form a mixed solution I, and adding a proper amount of seed cells into cartilage bio-ink to form a mixed solution II; and integrally constructing the composite bionic body surface tissue by using the mixed solution I and the mixed solution II. The method has the beneficial effects that the body surface tissue with the bilateral cartilage membranes and the middle cartilage tissue is constructed, the structure and composition characteristics of the body surface tissue containing the cartilage membranes are simulated, and different bionic scaffold materials and functional cells are utilized to realize mechanical bionics through structure and composition bionics, so that the mechanical strength of the construction material is improved, and the construction cost is reduced. The clinical application requirements of body surface tissue reconstruction are met.

Description

technical field [0001] The invention relates to the technical field of three-dimensional bioprinting and tissue and organ repair and reconstruction, in particular to a composite bionic body surface tissue and an integrated construction method thereof. Background technique [0002] Congenital microtia is the second largest craniofacial congenital deformity in my country, which seriously affects the physical and mental health of children. At present, the most effective treatment method is auricle reconstruction based on autologous costal cartilage carving, which is difficult, risky and has many postoperative complications. The rapid development of tissue engineering and regenerative medicine technology has brought new strategies for auricle reconstruction. At present, based on cartilage tissue engineering technology, polyglycolic acid / polylactic acid (PGA / PLA) and chondrocytes have successfully achieved the first international clinical breakthrough of tissue engineering auric...

Claims

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Application Information

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IPC IPC(8): A61L27/36A61L27/38A61L27/20A61L27/22A61L27/52B33Y10/00B33Y70/10B33Y80/00
CPCA61L27/3612A61L27/3687A61L27/3691A61L27/222A61L27/227A61L27/20A61L27/3804A61L27/3834A61L27/52B33Y70/10B33Y10/00B33Y80/00A61L2430/14
Inventor 蒋海越刘霞贾立涛
Owner PLASTIC SURGERY HOSPITAL CHINESE ACAD OF MEDICAL SCI
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