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Molecular marker closely linked with peanut ethylene insensitivity and salt tolerance related characters and application

An ethylene-insensitive, molecular marker technology, applied in the field of plant molecular genetics and molecular breeding, can solve the problem of unclear signal transduction pathway and specific mechanism of salt tolerance, achieve control of breeding scale, save production cost and cost low effect

Active Publication Date: 2022-04-22
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

A series of members of ethylene from receptors to downstream regulatory transcription factors are involved in the response process of plants to salt stress to varying degrees, and the ethylene induced by salt stress in turn positively regulates the response of plants to salt stress, but ethylene improves the salt tolerance of plants Sexual signal transduction pathways and specific mechanisms are still unclear (Yu Yanwen et al., 2013)
However, there is no report on the relationship between ethylene and peanut salt tolerance.

Method used

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  • Molecular marker closely linked with peanut ethylene insensitivity and salt tolerance related characters and application
  • Molecular marker closely linked with peanut ethylene insensitivity and salt tolerance related characters and application
  • Molecular marker closely linked with peanut ethylene insensitivity and salt tolerance related characters and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Example 1 Acquisition of Molecular Markers Linked to Peanut Ethylene Insensitivity and Salt Tolerance Genes

[0055] The mutant library was constructed by in vitro mutagenesis of peanut cultivar HY22 with Pingyangmycin, and the constructed mutants were obtained by referring to the method described in the invention patent "A method for directed selection of salt-tolerant body by in vitro mutagenesis of peanuts" application number 201310045768.6 The ethylene-insensitive mutant M29 with uninhibited hypocotyl elongation and better salt tolerance was screened from the library. The mutant was crossed with HY22, the hybrid was planted, and the F2 segregation population was obtained by selfing, which was used for the detection and positioning of ethylene-insensitive gene loci. The elongation of hypocotyls of mutant M29 and cultivar HY22 were treated with water, 50 μmol / L ACC and 0.7% NaCl respectively. Figure 1-3 Shown; Under water treatment, the hypocotyl elongation of mutan...

Embodiment 2

[0073] Method for rapidly cultivating ethylene-insensitive and salt-tolerant peanut varieties using molecular markers Arahy01063 and / or Arahy01102

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Abstract

The invention discloses a molecular marker closely linked with peanut ethylene insensitivity and salt tolerance related characters and application, and belongs to the technical field of plant molecular genetics and molecular breeding. The molecular marker closely linked with the ethylene insensitivity and salt tolerance related characters of the peanuts is an InDel marker Arahy01063 and / or Arahy01102, and the molecular marker is an InDel marker Arahy01063 and / or Arahy01102. The molecular marker provided by the invention can realize rapid detection of ethylene insensitivity and salt tolerance of peanuts, eliminates the influence of the environment, and has important application value in peanut molecular breeding and acceleration of the cultivation process of peanut salt-tolerant varieties.

Description

technical field [0001] The invention belongs to the technical field of plant molecular genetics and molecular breeding, and specifically relates to a molecular marker closely linked with peanut ethylene insensitivity and salt tolerance related traits and its application. Background technique [0002] Peanut (Arachis hypogaea L.), as an important oil crop and economic crop in the world, plays an important role in combating malnutrition and ensuring food security. In recent years, domestic and foreign scholars have made breakthroughs in the cultivation of new varieties of peanuts, the utilization of wild germplasm resources, and molecular design breeding. huge contribution. Salt stress will inhibit the growth of peanuts, cause the plants to wilt, reduce the yield, and at the same time make the quality of the pods deteriorate, and the commercial value will be significantly reduced. With the increasing soil salinization, creating new salt-tolerant peanut germplasm, mining salt...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12Q1/686C12N15/11A01H1/02A01H1/04
CPCC12Q1/6895C12Q1/686A01H1/021A01H1/04C12Q2600/13C12Q2565/125
Inventor 唐艳艳李鑫胡畅丽周文杰孙明明乔利仙朱虹隋炯明王晶珊刘文平
Owner QINGDAO AGRI UNIV
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