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A pesticide intermediate degrading bacterial strain, its cultivation method and its bacterial agent and application

A technology for intermediates and pesticides, applied in the field of pesticide intermediates degrading strains, can solve problems such as refractory biodegradation, accumulation, environmental and human health hazards, and achieve the effects of high-efficiency degradation capacity, simple processing technology, and low processing costs.

Active Publication Date: 2022-06-03
江苏朴厚环境工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These pesticides and their intermediates are easy to accumulate in organisms and are difficult to biodegrade. The harm to the environment and human health cannot be ignored.

Method used

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  • A pesticide intermediate degrading bacterial strain, its cultivation method and its bacterial agent and application
  • A pesticide intermediate degrading bacterial strain, its cultivation method and its bacterial agent and application
  • A pesticide intermediate degrading bacterial strain, its cultivation method and its bacterial agent and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Isolation, screening and identification of degrading strain PH-1:

[0041] 1. Isolation and screening of degrading strain PH-1

[0042]The invention provides a degrading strain PH-1 for efficiently degrading pesticides, which is separated from the activated sludge of a sewage treatment plant. The specific strain culture method is as follows: take the activated sludge in the biochemical tank of the sewage treatment plant, add it to the inorganic salt medium containing CCMP for domestication and culture; Cultured with constant temperature shaking at 160 rpm; after culturing for 5 days, the inoculum of 5% (V / V) was inserted into fresh inorganic salt medium, and enriched and transferred 4 times in a row; 1 mL of enrichment solution was diluted with sterile water To different concentration gradients (10-2-10-6), take 0.2 mL of dilutions of different concentration gradients, spread them on R2A agar medium plates containing 100 mg / L CCMP, and place them in a 30°C constant tem...

Embodiment 2

[0048] Degradation effect of degrading strain PH-1:

[0049] The single colony of degrading strain PH-1 obtained by screening in Example 1 was inoculated in 50 mL of LB medium, enriched and cultured to OD600=1.0 under the conditions of 30 ° C and 160 rpm, and the obtained culture solution was centrifuged at 4 ° C and 10000g for 10 min, The bacteria were collected, washed three times with sterile phosphate buffer and resuspended in phosphate buffer as a bacterial suspension. The obtained bacterial suspension was inoculated into an inorganic salt medium with CCMP as the sole carbon source at a volume ratio of 5%, the CCMP concentration in the culture solution was 500 mg / L, and the culture was incubated at 30 ° C and 160 rpm under constant temperature shaking for 84 h, and sampling was performed regularly. The corresponding OD600 value and the concentration of CCMP in the culture medium were determined, and the experiment was repeated three times. To draw the degradation and gro...

Embodiment 3

[0052] The degradation effect of degrading strain PH-1 on the downstream products of CCMP:

[0053] The inorganic salt medium of imidacloprid, fluazinam and diflufenapyr with a final concentration of 200 mg / L was prepared respectively, and the pH of the medium was adjusted to 7. Pick the single colony of degrading strain PH-1 obtained by screening in Example 1, inoculate it in 50mL LB medium, enrich and cultivate it to OD600=1.0 under the conditions of 30°C and 160rpm, and the obtained bacterial liquid is 5% by volume (V / V ) were inoculated into the medium containing different pesticides, cultured in a constant temperature shaking box at 30 °C and 160 rpm, and the degradation was detected after 72 h. The degradation effect is as follows: Figure 4 shown.

[0054] according to Figure 4 It can be seen that the strain PH-1 can effectively degrade the downstream products of 2-chloro-5-chloromethylpyridine, and the degradation rates of imidacloprid, flufenazuron and diflufenacil...

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Abstract

The invention discloses a pesticide intermediate degrading strain. The degrading strain is Achromobacter denitrificans PH‑1, which was preserved in the China Ordinary Microorganism Strain Preservation Management Center on December 6, 2021. The strain preservation number is For: CGMCC NO.24038. The pesticide intermediate degrading bacterial strain PH-1 of the present invention can tolerate high concentration of sodium chloride, and is effective against organic pollutants 2-chloro-5-chloromethylpyridine and imidacloprid, fluazinam, and pyridine in pesticide wastewater. Downstream products such as fluazifop-methyl have high-efficiency degradation ability.

Description

technical field [0001] The invention relates to the technical field of pesticide biodegradation, in particular to a pesticide intermediate degrading bacterial strain, a culture method, a bacterial agent and application thereof. Background technique [0002] In the pesticide production process, a large amount of pesticide wastewater is produced, mainly from the wastewater generated during the pesticide production process and the wastewater generated from the cleaning of production equipment. The wastewater has a high concentration of organic pollutants, many by-products and intermediates, high salinity, and the pesticide wastewater is very toxic. In addition to pesticides and intermediates, it also contains toxic substances such as benzene, phenol, mercury, and many biodegradable substances. In addition, the problems of pesticide waste water quality and water quantity instability increase the difficulty of its treatment. If it is not treated effectively, it will seriously dam...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/025C02F101/30C02F101/36C02F101/38
CPCC12N1/20C02F3/34C02F2101/306C02F2101/36C02F2101/38Y02W10/10
Inventor 董晓梦邱沪生吴康莉杨倩
Owner 江苏朴厚环境工程有限公司
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