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Preparation method of immobilized enzyme for degrading aflatoxin B1 in peanuts

A technology of aflatoxins and immobilized enzymes, applied to biochemical equipment and methods, oxidoreductases, immobilized on or in inorganic carriers, etc., can solve the difficulty of separation and recovery, increase production costs, and cannot be reused multiple times and other issues, to achieve the effects of efficient degradation, lower production costs, high market value and application potential

Pending Publication Date: 2022-05-10
安徽黑娃食品生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in most cases laccases degrade aflatoxin B 1 The specific catalytic reaction is carried out in aqueous solution. After the reaction, it remains in the solution. It is difficult to separate and recover, and it cannot be reused many times, which increases the production cost. This has become the main limitation of its industrial application.

Method used

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  • Preparation method of immobilized enzyme for degrading aflatoxin B1 in peanuts
  • Preparation method of immobilized enzyme for degrading aflatoxin B1 in peanuts

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Step 1. Magnetic Chitosan Composite Particle Fe 3 o 4 Preparation of @CS material

[0026] 6.12 g of ferrous sulfate heptahydrate and 11.88 g of ferric chloride hexahydrate were dissolved in 100 mL of deionized water, and sodium hydroxide was added to adjust the pH to 10.0. Under nitrogen protection, the mixture was stirred vigorously at 80 °C for one hour. After cooling to room temperature, the precipitate was separated with a magnet, that is, Fe 3 o 4 , and washed with deionized water. 5g of iron oxide was diffused into 1000mL of chitosan solution with a concentration of 6.0mg / mL, then, 500mL of triphenyl phosphate solution with a concentration of 1.0mg / mL was added, and treated by ultrasonic wave at room temperature for 60min. Separation of Magnetic Chitosan Composite Microparticle Fe by Magnet 3 o 4 @CS, and dried under vacuum at 40 °C after washing with ethanol.

[0027] Step 2. Immobilization of laccase

[0028] The 5g magnetic chitosan composite particle...

Embodiment 2

[0032] Step 1. Magnetic Chitosan Composite Particle Fe 3 o 4 Preparation of @CS material

[0033] 6.12 g of ferrous sulfate heptahydrate and 11.88 g of ferric chloride hexahydrate were dissolved in 100 mL of deionized water, and sodium hydroxide was added to adjust the pH to 10.0. Under nitrogen protection, the mixture was stirred vigorously at 80 °C for one hour. After cooling to room temperature, the precipitate was separated with a magnet, that is, Fe 3 o 4 , and washed with deionized water. 5g of iron oxide was diffused into 1000mL of chitosan solution with a concentration of 6.0mg / mL, then, 500mL of triphenyl phosphate solution with a concentration of 1.0mg / mL was added, and treated by ultrasonic wave at room temperature for 60min. Separation of Magnetic Chitosan Composite Microparticle Fe by Magnet 3 o 4 @CS, and dried under vacuum at 40 °C after washing with ethanol.

[0034] Step 2. Immobilization of laccase

[0035] The 5g magnetic chitosan composite particle...

Embodiment 3

[0039] Step 1. Magnetic Chitosan Composite Particle Fe 3 o 4 Preparation of @CS material

[0040] 6.12 g of ferrous sulfate heptahydrate and 11.88 g of ferric chloride hexahydrate were dissolved in 100 mL of deionized water, and sodium hydroxide was added to adjust the pH to 10.0. Under nitrogen protection, the mixture was stirred vigorously at 80 °C for one hour. After cooling to room temperature, the precipitate was separated with a magnet, that is, Fe 3 o 4 , and washed with deionized water. 5g of iron oxide was diffused into 1000mL of chitosan solution with a concentration of 6.0mg / mL, then, 500mL of triphenyl phosphate solution with a concentration of 1.0mg / mL was added, and treated by ultrasonic wave at room temperature for 60min. Separation of Magnetic Chitosan Composite Microparticle Fe by Magnet 3 o 4 @CS, and dried under vacuum at 40 °C after washing with ethanol.

[0041] Step 2. Immobilization of laccase

[0042] The 5g magnetic chitosan composite particle...

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Abstract

The invention relates to a preparation method of an immobilized enzyme for degrading aflatoxin B1 in peanuts, and belongs to the technical field of degradation of aflatoxin B1. Comprising the following steps: mixing ferric chloride and ferrous chloride, adding ammonia water or sodium hydroxide as a precipitant, enabling Fe < 3 + > and Fe < 2 + > to generate precipitates in the form of hydrated oxide or hydroxide, and finally obtaining the Fe3O4 magnetic microspheres. Dissolving Fe3O4 into a chitosan solution, adding a triphenyl phosphate solution, and performing ultrasonic treatment; and separating the magnetic chitosan composite particles Fe3O4 (at) CS with a magnet, washing with ethanol for several times, and drying in a vacuum drying oven to obtain the immobilized carrier. According to the method disclosed by the invention, the laccase is immobilized by using the magnetic chitosan composite particles Fe3O4 (at) CS, so that the aflatoxin in the peanuts can be efficiently degraded, and rapid recovery and reutilization of the laccase after complete reaction can be realized.

Description

technical field [0001] The invention belongs to aflatoxin B 1 Degradation technical field, specifically relate to a kind of degrading aflatoxin B in peanut 1 Preparation method of immobilized enzyme. Background technique [0002] Aflatoxins (AFs) are common mycotoxins in corn, peanuts and other grains. They are one of the strongest chemical carcinogens found so far. They mainly cause liver cancer by damaging the liver, and can also induce rectal cancer and breast cancer. and bone cancer, among which aflatoxin B 1 (AFB 1 ) is the most toxic. [0003] Aflatoxin B 1 The detoxification methods mainly include chemical, physical and biological methods. Chemical method is to use acid, alkali, oxidizing agent, aldehyde or sulfurous acid gas to change aflatoxin B 1 The disadvantage is that it may affect the nutritional value and flavor of food, and there are potential safety hazards of chemical residues. The physical detoxification method uses adsorbents to remove toxins. The...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/10C12N11/14C12N9/02A23L5/20
CPCC12N11/10C12N11/14C12N9/0061C12Y110/03002A23L5/25
Inventor 魏秀兰秋杨煜崔晓冰魏长香王景源何楠楠夏雨
Owner 安徽黑娃食品生物科技有限公司