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Polydopamine-coated slow-release MnO2 nano-microsphere drug loading system

A nano-microsphere and drug-loading system technology, applied in the field of biomedicine, can solve the problems of low selection specificity, high toxicity and side effects, and application limitations, and achieve the effects of good biocompatibility, high targeting, and convenient operation

Active Publication Date: 2022-05-13
ZHEJIANG CANCER HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Doxorubicin (DOX) is a small-molecule anti-cancer drug that has significant effects on many types of tumors in the human body, but the selection specificity is low, and the toxicity and side effects are relatively large, so the application is greatly limited

Method used

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  • Polydopamine-coated slow-release MnO2 nano-microsphere drug loading system
  • Polydopamine-coated slow-release MnO2 nano-microsphere drug loading system
  • Polydopamine-coated slow-release MnO2 nano-microsphere drug loading system

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preparation example Construction

[0075] Provide the aforementioned slow-release MnO 2 A method for preparing a novel drug-loading system of nano-microspheres, including:

[0076] Step 1: Transfer the glucose solution with a concentration of 0.01-0.5M to a tetrafluoroethylene high-pressure reactor, and conduct a hydrothermal reaction at a temperature of 150-300°C for at least 12 hours to obtain a tan precipitate, which is washed several times with deionized water and absolute ethanol, Drying in a blast oven at 60-90°C to obtain carbon nanospheres;

[0077] Step 2: Ultrasonic dispersion of carbon nanospheres in deionized water to obtain a water dispersion of carbon nanospheres, adjusted to 1-5 with concentrated HCl, according to the concentration of carbon nanospheres and KMnO 4 Add KMnO in powder weight ratio 1:2-4 4 Powder, continue ultrasonic dispersion for at least 10min, react at 80-150℃ for 30min-12h, generate MnO in situ 2 , washed with deionized water and absolute ethanol, dried, and sintered at 200-...

Embodiment 1

[0089] Provides a slow-release MnO 2 A novel drug-loading system for nano-microspheres, specifically pH-controlled release and folic acid-targeted drug-loaded MnO 2 Nanocomposite hollow spheres, the preparation process of which is as follows figure 1 As shown, the preparation method includes the following steps.

[0090](1) Preparation of nano-carbon spheres: magnetically stir 0.1M 30mL glucose solution evenly, transfer it to a 50mL tetrafluoroethylene autoclave, and conduct a hydrothermal reaction at 180°C for 15h; wash the obtained tan precipitate with deionized water and anhydrous ethanol for 3 times, and dried in a blast oven at 80°C to constant weight to obtain carbon nanospheres.

[0091] (2) Preparation of MnO 2 Nano hollow sphere: Ultrasonic (35KHz, 0.4W / cm2) 0.042g nano carbon sphere 2 ) was dispersed in 20 mL of deionized water, and the pH was adjusted to 2.5 with concentrated HCl; then 0.111 g of KMnO was added to the suspension 4 Powder, continue to ultrasonic...

Embodiment 2

[0096] Characterization of the properties of nanomaterials. The potential of nanomedicines at different pH values ​​(5.0 and 7.4) was tested using a Malvern nanoparticle size analyzer. The result is as Figure 6 As shown, at pH=7.4 (simulating the blood environment in the body), all the nanomaterials prepared are negatively charged, indicating that the negatively charged nanomedicine can avoid the adsorption of proteins in the body, reduce the toxic effect of the drug in the body, and prolong the life of the drug. Drug circulation cycle in vivo; at pH=5.0 (simulating tumor microenvironment), MnO 2 @DOX is modified with -NH after being coated with PDA 2 MnO 2 @DOX-NH 2 Showing strong electropositivity and realizing charge reversal, the final MnO 2 @DOX-NH 2 -FA composite nano-drugs are positively charged, which helps nano-drugs gather to the negatively charged tumor cells on the surface, so that the drug can be targeted and concentrated, which facilitates targeted drug del...

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Abstract

The invention relates to the technical field of biomedicine, in particular to a polydopamine-coated slow-release MnO2 nano-microsphere drug loading system which comprises a MnO2 inorganic nano-particle carrier and is specifically prepared by taking an inorganic nano-oxide MnO2 hollow sphere as a nano-drug platform, wrapping the nano-hollow sphere by utilizing PDA (Personal Digital Assistant) and taking FA (Fatty Acid) as a targeting probe. The chemotherapeutic drug DOX is gathered at a tumor part, and the curative effect of the drug is enhanced. According to the invention, the biocompatibility is obviously improved, the specific targeting of the medicine is enhanced, tumor cells are effectively killed, and the damage to normal cells is reduced. The nano-carrier is modified, so that the pH response sensitivity is improved, the effective concentration of the medicine in blood is prolonged, the nano-carrier can be gradually decomposed in vivo and discharged out of the body, and the nano-carrier is an ideal medicine carrying device for cancer treatment.

Description

technical field [0001] The invention relates to an anti-tumor nano drug and its preparation method and application, belonging to the technical field of biomedicine, in particular to a drug-loaded MnO with pH controllable release and folic acid targeting 2 Nanocomposite hollow spheres. Background technique [0002] In 2020, breast cancer has surpassed lung cancer to become the largest cancer in the world. Chemotherapy is the cornerstone of breast cancer treatment. Previous EBCTCG Chemotherapy Highlights: Anthracyclines lay the "core" of breast cancer chemotherapy, but cardiotoxicity limits the use of patients. It is particularly important to develop new and highly effective cancer treatments with tumor targeting and reduced toxicity to normal tissues. [0003] In recent years, with the continuous development of nanotechnology, it is usually used as an ideal carrier for tumor drugs due to its advantages such as good biocompatibility, long in vivo cycle and strong adsorption...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/69A61K47/52A61K47/54A61K31/704A61K45/00A61K47/34A61P35/00B82Y5/00
CPCA61K47/6925A61K47/6949A61K47/52A61K47/545A61K47/34A61K31/704A61K45/00A61P35/00B82Y5/00
Inventor 陈占红王晓稼陈俊青
Owner ZHEJIANG CANCER HOSPITAL
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