Quinolizidine alkaloid as well as preparation method and application thereof

A technology for quinolizidine and alkaloids, which is applied in the field of quinolizidine alkaloids and their preparation, can solve the problems of low yield, lack of resistance of disease-resistant varieties, residues and the like, and achieves a simple and easy preparation method. Manipulation, strong anti-TSWV activity, and novel structural effects

Active Publication Date: 2022-05-13
THE KEY LAB OF CHEM FOR NATURAL PROD OF GUIZHOU PROVINCE & CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In the prior art, measures such as spraying anti-plant virus chemical agents and planting disease-resistant varieties are mainly used to prevent and treat TSWV, but none of them can solve the problem of TSWV infection well.
Although most of the chemical agents used can inhibit or reduce the proliferation of TSWV virus in plants, they cannot eradicate the virus, and even some chemical agents will cause phytotoxicity and residues on tobacco leaves; and disease-resistant varieties are also prone to resistance due to fewer resources The lack of other problems and the effect is not high
So far, no chemical pesticides or measures that can completely eradicate or effectively control TSWV have been found

Method used

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  • Quinolizidine alkaloid as well as preparation method and application thereof
  • Quinolizidine alkaloid as well as preparation method and application thereof
  • Quinolizidine alkaloid as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0047] A quinolizidine alkaloid, wherein the quinolizidine alkaloid is selected from any one of compounds 1 to 7 of the following chemical structures:

[0048]

[0049] The preparation method of quinolizidine alkaloids comprises the following steps:

[0050] (1) Take 50 kg of dried Cassia lanceolata seeds and extract them 3 times with methanol reflux, 2 to 3 hours each time, combine the extracts and concentrate to obtain extracts, which are treated with acid and alkali, that is, first use 3% hydrochloric acid Dissolve, then extract with petroleum ether to remove non-alkaloid components, adjust the pH of the acidic water layer to 9-10 with 25% concentrated ammonia water, and then extract the alkaline water layer with dichloromethane to obtain 3.5 kg of total alkaloids;

[0051] (2) The total alkaloids were subjected to 300-400 mesh silica gel column chromatography, and gradient elution was carried out with dichloromethane:methanol with a volume ratio of 100:0-0:100 as the mo...

experiment example 1

[0059] The quinolizidine alkaloid that embodiment obtains is carried out anti-TSWV active research verification, and specific method is as follows:

[0060] a. The host is common tobacco cultivar K326 (Nicotiana tabacum cv. K326), and the seeds are preserved and provided by this experiment and cultivated in a glass greenhouse. The source of tomato spotted wilt virus (TSWV) was provided by our laboratory. Ningnanmycin was purchased from Heilongjiang Deqiang Biological Co., Ltd.

[0061] TSWV was stored in a -80°C refrigerator in our laboratory.

[0062] b. Virus inoculation and drug application:

[0063] Inoculate the virus first and then apply the drug: select 5-6 tobacco seedlings at the true leaf stage with consistent growth to rub and inoculate TSWV, inoculate TSWV on the 4th and 5th true leaves, rinse with water after 10 minutes, and then apply them separately after 24 hours 1 mL of compound 1 to compound 7 with a concentration of 100 μg / mL, spread evenly, and rinse wit...

experiment example 2

[0070] The insecticidal activity of the quinolizidine alkaloids of the embodiment is researched and verified, specifically as follows:

[0071] Targets for testing: Aphis fabae is a sensitive strain that has been raised indoors with broad bean seedlings for many years, and the test insects are 3-day-old nymphs; cotton red spider (Tetranychus urticae) is a sensitive strain that has been raised indoors with broad bean seedlings for many years. The test insects were healthy adult mites.

[0072] Culture conditions: the culture conditions of the tested target and the target after the test are temperature of 25±5°C, relative humidity of 65±5%, and photoperiod of 12 / 12h (L / D).

[0073] Instruments and equipment: electronic balance (sense of 1 / 10,000), spray tower, 100ml beaker, glass test tube, graduated cylinder, Petri dish, paramembrane, sponge, filter paper, pipette, tweezers, brush, microscope, etc.

[0074] Test medicaments: Compound 1 to Compound 7 in the examples, the content ...

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Abstract

The invention discloses quinolizidine alkaloid as well as a preparation method and application thereof, the quinolizidine alkaloid is selected from any one of new compounds 1-7, and the quinolizidine alkaloid is extracted and separated from seeds of wild cassia lanceolata or is obtained through artificial synthesis. The invention further discloses a preparation method of the quinolizidine alkaloid, and the quinolizidine alkaloid is verified to have a remarkable effect on prevention and treatment of tomato spotted wilt virus and pest killing.

Description

technical field [0001] The invention relates to the technical field of pesticides for crops, in particular to a quinolizidine alkaloid and a preparation method and application thereof. Background technique [0002] Plant virus diseases have caused serious losses to crop production in my country and around the world. Plant virus is a special kind of molecular parasite, which does not have any cell structure itself, and needs to rely on the host's cytokines and cell structure to complete its own biological processes such as replication, movement, packaging, and pathogenicity. During this series of processes, the normal growth and development of plants are often severely disturbed, and even lead to the death of plants. [0003] Tomato spotted wilt virus (Tomato spotted wilt virus, TSWV) is a representative member of the Bunyaviridae family Spotted wilt virus, and is an RNA virus. The virus has the characteristics of wide distribution, strong infectivity and high lethality, an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D471/04C07D471/22C07D519/00C07D491/22A01N43/90A01P7/04A01P1/00
CPCC07D471/04C07D471/22C07D519/00C07D491/22A01N43/90Y02A50/30
Inventor 胡占兴郝小江赵立华安巧张鹏邹吉斌
Owner THE KEY LAB OF CHEM FOR NATURAL PROD OF GUIZHOU PROVINCE & CHINESE ACADEMY OF SCI
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