Antibody complex, biosensor and ECL detection platform and detection method of SARS-CoV-2
A biosensor, antibody complex technology, applied in chemical instruments and methods, nanotechnology for sensing, biomaterial analysis, etc. The effect of improving ECL emission and improving detection sensitivity
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Embodiment 1
[0082] This embodiment provides a β-CD-Pt nanocomposite and its preparation method.
[0083] 1.0 mg β-CD was dissolved in deionized water and subjected to vigorous sonication to form a 1.0 mg / mL β-CD solution. Then, 10 μL H 2 PtCl 6 (1%) was added to the β-CD solution and stirred vigorously overnight. Subsequently, 25 μL NaBH 4 (0.01M) was slowly added to the above mixed solution, and reacted for 20 minutes under stirring conditions. Continue to add 10 μL of sodium citrate (0.01M), continue stirring for 30 minutes to form a β-CD-Pt nanocomposite material, centrifuge at 10,000 rpm, wash with water for 3 times and collect. Finally, the collected β-CD-Pt was dispersed in 1.0 mL of water to obtain a dispersion thereof.
[0084] As a control, Pt nanoparticles (PtNPs) were prepared according to the same reduction method described above in the absence of β-CD.
Embodiment 2
[0086] This embodiment provides an antibody complex and its preparation method.
[0087] Preparation reference of TDBA-β-CD-Pt@Ab2 complex figure 1 Shown in B.
[0088] The preparation process of the secondary antibody complex (TDBA-β-CD-Pt@Ab2) as shown in the principle figure 1 Shown in B. Briefly, 4.0 μL TDBA, 400 μL β-CD-Pt dispersion and 40 μL Ab2 (50 μg / mL) were mixed and reacted at 4.0 °C for 14 h to form TDBA-β-CD-Pt@Ab 2 nanocomposites. Then, TDBA-β-CD-Pt@Ab was blocked by incubating the nanocomposite with BSA (1%, 100 μL) for 40 min 2 remaining active sites. Finally, the obtained TDBA-β-CD-Pt@Ab 2 Centrifuged at 8000 rpm, washed 3 times with 0.10M PBS (pH 7.4), and further dispersed in 400 μL of 0.10M PBS (pH 7.4), and stored at 4.0°C for later use.
Embodiment 3
[0090] This embodiment provides a preparation method of PDP PNPs.
[0091] First, 5.0 mg of PDP and 1.0 mg of PSMA were dissolved in tetrahydrofuran (THF) to prepare respective solutions (1.0 mg / mL). Subsequently, the obtained PDP and PSMA solutions were well mixed for 2.0 hours, and deionized water (10 mL) was further injected. Next, the resulting mixed solution was heated at 80° C. in an air atmosphere until THF was completely evaporated to obtain a PDP PNPs dispersion. principle figure 1 Middle A plots the preparation process of PDP PNPs.
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