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Staphylococcus equorum and application of staphylococcus equorum in prevention and treatment of gray mold of fruits and vegetables

A technology for staphylococcus and botrytis cinerea, applied in the field of microorganisms, can solve the problems of lack of biocontrol agents, few biological strains, etc., and achieve the effects of improving the expression level of disease resistance genes, increasing enzyme activity, and excellent antagonism.

Active Publication Date: 2022-05-20
QINGDAO AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, there are few biological strains that can be used to prepare biocontrol agents for preventing and treating Botrytis cinerea, and there is a lack of corresponding effective biocontrol agents. Therefore, how to solve the above technical problems is a problem that needs to be solved in the field of microbial technology. technical issues

Method used

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  • Staphylococcus equorum and application of staphylococcus equorum in prevention and treatment of gray mold of fruits and vegetables
  • Staphylococcus equorum and application of staphylococcus equorum in prevention and treatment of gray mold of fruits and vegetables
  • Staphylococcus equorum and application of staphylococcus equorum in prevention and treatment of gray mold of fruits and vegetables

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Example 1 Isolation, screening and strain identification of Staphylococcus equisetum F1

[0047] 1. Isolation and screening of strains

[0048] Mature and healthy fruits were collected from a commercial Fuji apple orchard in Shandong, and after surface disinfection, the fruit tissue was ground, added with sterile water, and the conventional gradient dilution coating separation method was used. The colonies with obvious morphological differences were purified and stored on PDA medium, and the primary screening and repeated screening of antagonistic bacteria were carried out with the apple tree rot pathogen as the target pathogen, and a bacterial strain with bacteriostatic effect and significant control effect was finally obtained. , and name it F1.

[0049] 2. Identification of strain F1

[0050] (1) Morphological characteristics

[0051] like figure 1 The results showed that the colony of strain F1 on the LB medium was small, grayish-white, rounded (slightly irregul...

Embodiment 2

[0059] Example 2 The effect of Staphylococcus equisetum F1 on mycelial growth and spore germination of Botrytis cinerea on fruits and vegetables

[0060] (1) The effect of strain F1 on the growth of mycelium of Botrytis cinerea

[0061] A. Experimental method: The seed fermentation broth of Staphylococcus equorum F1 was inoculated into PDB medium, and incubated at 28°C with constant temperature shaking for 48 hours to obtain the cell culture liquid of strain F1, and the cells of strain F1 were collected after centrifugation. Prepare PDA medium, add the bacterial cells of strain F1 to it, and make the final concentration of 10 5 , 10 7 and 10 9 cfu·mL -1 , with PDA without strain F1 as a control. The above-mentioned PDA medium was inoculated with activated Botrytis cinerea. The cells were cultured at 25°C in the dark for 3 days, and the colony diameter was observed and measured.

[0062] B. Results and analysis:

[0063] The results showed that the addition of different ...

Embodiment 3

[0069] Example 3 Inhibitory effect of Staphylococcus equisetum F1 on various plant pathogens

[0070] A. Experimental method: prepare Staphylococcus equorum F1 bacterial suspension to make the final concentration 10 9 cfu·mL -1 , with PDA without strain F1 as a control, and then inoculated with activated cultured pathogens.

[0071] Bacteriostatic rate=(colony diameter of control group-colony diameter of treatment group) / colony diameter of control group×100%.

[0072] B. Results and analysis: As shown in Table 1, strain F1 has significant inhibitory effect on major plant pathogens such as Botrytis cinerea, apple tree rot, apple rot, and apple anthracnose leaf blight, showing good results. The broad-spectrum antibacterial properties of the bacteria were shown in Table 1.

[0073] Table 1 Inhibitory effect of Staphylococcus equorum strain F1 on major plant pathogens

[0074]

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Abstract

The invention discloses a staphylococcus equorum strain and application thereof, the staphylococcus equorum strain is named as staphylococcus equorum F1, the staphylococcus equorum strain is preserved in China General Microbiological Culture Collection Center on January 18, 2021, the preservation number of the staphylococcus equorum strain is CGMCC No.21658, and the preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The staphylococcus equorum F1 has an efficient antibacterial effect on fruit and vegetable botrytis cinerea, can significantly inhibit growth of botrytis cinerea hyphae and germination of spores, can be applied to preparation of biocontrol inoculants and fruit and vegetable preservatives, has the characteristics of wide antibacterial spectrum, stable control effect, environmental friendliness and the like, and is suitable for large-scale production and low in production cost.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a strain of Staphylococcus equisetum and its application in preventing and treating botrytis cinerea of ​​fruits and vegetables. Background technique [0002] Botrytis cinerea caused by Botrytis cinerea is one of the important diseases during postharvest storage of fruits and vegetables. After fruits and vegetables are infected with gray mold, circular or nearly circular water-soaked soft rot lesions are formed at the diseased site, and rat gray mold and conidia are produced on the later lesions. Because the pathogen has strong low temperature resistance, it can cause fruit and vegetables to rot even under refrigeration conditions, and there is contact infection. Effective control of Botrytis cinerea is of great significance for improving postharvest storage and preservation of fruits and vegetables. [0003] At present, the traditional methods of preventing and controll...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/20A01P3/00A23B7/155C12R1/44
CPCC12N1/20A01N63/20A23B7/155A23V2002/00A23V2200/10
Inventor 王彩霞郝柏慧赵润欣孙子豪李保华
Owner QINGDAO AGRI UNIV
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