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Method for precisely and completely dechlorinating chlorinated hydrocarbon based on slow-release carbon source coupled bioelectrochemical system

A technology of bioelectrochemistry and chlorinated hydrocarbons, applied in chemical instruments and methods, electrochemical biocombination treatment, sustainable biological treatment, etc., can solve the problem of weak competition ability of dechlorinated respiratory bacteria, production of toxic intermediate products, electron supply, etc. In order to reduce the biological risk, achieve efficient utilization and prevent excessive proliferation

Active Publication Date: 2022-05-24
HARBIN INST OF TECH
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Problems solved by technology

[0004] Based on the above, in order to solve the problems of lack of carbon source, lack of electron donor, weak competitive ability of dechlorination respiratory bacteria, and production of toxic intermediate products in the process of anaerobic biological dechlorination of chlorinated hydrocarbons, the present invention proposes a method of slow-release carbon source A method for precise dechlorination of volatile chlorinated hydrocarbons to non-toxic and harmless ethylene by coupling bioelectrochemical system

Method used

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  • Method for precisely and completely dechlorinating chlorinated hydrocarbon based on slow-release carbon source coupled bioelectrochemical system
  • Method for precisely and completely dechlorinating chlorinated hydrocarbon based on slow-release carbon source coupled bioelectrochemical system
  • Method for precisely and completely dechlorinating chlorinated hydrocarbon based on slow-release carbon source coupled bioelectrochemical system

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Embodiment 1

[0036] The specific operation process of promoting the complete dechlorination of chlorinated hydrocarbons based on the slow-release carbon source coupled with bioelectrochemistry is as follows:

[0037] 1. Research on the carbon release capacity of slow-release carbon source PHB:

[0038] After sterilization, 80 mL of sterile water and 2.0PHB were added to the 100 mL anaerobic bottle to seal, and two groups of parallel experiments and blank experiments were set up (only 80 mL of sterile water was added to the 100 mL anaerobic bottle). Each group of anaerobic flasks was placed in a constant temperature shaking incubator (25±1℃, 70rpm), and samples were taken every 1 d to detect COD. According to the carbon release situation in a single day, the dosage of carbon source was preliminarily determined.

[0039] Second, configure the anaerobic culture medium:

[0040] According to the proportion of each 1L anaerobic culture solution including the following components: 11.55gNa 2 H...

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Abstract

The invention discloses a method for precisely and completely dechlorinating chlorinated hydrocarbon based on a slow-release carbon source coupled bioelectrochemical system, and belongs to the technical field of in-situ bioremediation of soil and underground water. According to the invention, the problem of accumulation of toxic and harmful intermediate products caused by incomplete chlorohydrocarbon degradation due to lack of exogenous organic carbon and electron donors in the anaerobic microorganism reductive dechlorination process in the site chlorohydrocarbon pollution in-situ bioremediation process is solved; the problems of site COD (Chemical Oxygen Demand) increase and secondary pollution caused by conventional addition of the water-soluble organic carbon source are solved. According to the method, PHB with efficient selectivity is adopted as a slow-release carbon source, a PHB coupled bioelectrochemical system is constructed, and under the dual electron supply action of electron release of a biological cathode and hydrogen release of PHB, a volatile chlorinated hydrocarbon (trichloro ethylene (TCE) / tetrachloroethylene (PCE) and the like) dechlorination functional flora is selectively activated, sequential dechlorination of PCE and TCE to ethylene is accurately promoted, and the purpose of dechlorinating ethylene is achieved. The efficient and complete dechlorination and detoxification of the chlorinated hydrocarbon are realized.

Description

technical field [0001] The invention relates to a method for accurate and complete dechlorination of chlorinated hydrocarbons based on a slow-release carbon source coupled bioelectrochemical system, and belongs to the technical field of soil and groundwater in-situ bioremediation. Background technique [0002] Chlorinated hydrocarbons are widely used in industry as common organic solvents, dry cleaning agents, and explosion-proof agents. In recent years, due to the extensive use and improper treatment of chlorinated hydrocarbons, a large number of chlorinated organic pollutants have entered the environment, causing environmental pollution. Among them, once chlorinated hydrocarbons enter the groundwater to form heavy non-aqueous fluid pollution plumes, it will pose a serious threat to human health and ecological security. [0003] At present, the methods for the removal of chlorinated hydrocarbons in groundwater at home and abroad mainly include physical and chemical technol...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/00C02F3/34C02F101/36
CPCC02F3/005C02F3/348C02F2101/36C02F2305/06C02F2303/14Y02W10/10
Inventor 李智灵柏才华王爱杰陈雪琪俎云霞刘衬衬曹迪程浩毅
Owner HARBIN INST OF TECH
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