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Method for constructing engineered intelligent myocardial tissue based on fish scale substrate

An engineering and fish-scale technology, applied in the field of biomedical engineering, can solve the problems of complex and cumbersome process and high cost, and achieve the effect of abundant sources, low cost, and improving the efficiency of myocardial tissue structure and functional maturation

Pending Publication Date: 2022-05-27
SUZHOU FISHSEEDS BIOLOGICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Mechanical stimulation to simulate heart contraction is also one of the effective methods, but the current mechanical stimulation method requires an elastic culture substrate, the culture substrate needs to be connected to the stretching device, and the cells or tissues need to be repeatedly removed from the incubator. These processes are complicated. cumbersome and expensive

Method used

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  • Method for constructing engineered intelligent myocardial tissue based on fish scale substrate
  • Method for constructing engineered intelligent myocardial tissue based on fish scale substrate
  • Method for constructing engineered intelligent myocardial tissue based on fish scale substrate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1 Construction of engineered intelligent myocardial tissue with shad scale base

[0039] A method for constructing an engineered intelligent myocardial tissue on a shad scale base, comprising the following steps:

[0040] (1) Manually tear 3-5 pieces of fish scales from the fresh anchovies, and wash them with clean water (the photo is as shown in figure 1 shown).

[0041] (2) Soak the shad scales in 1 M sodium hydroxide solution for 48 hours; wash the shad scales 4 times with deionized water.

[0042](3) Soak the shad scales in 0.5 M ethylenediaminetetraacetic acid solution for 72 hours; wash the shad scales with deionized water 4 times and dry them for later use (the photos are as follows: figure 2 shown).

[0043] (4), soak the polyethylene film in 0.3% Ti 3 C 2 Tx MXenes in ethanol solution for Ti 3 C 2 Tx MXenes were adsorbed on the surface of the film and then taken out to dry to obtain MXenes polyethylene film.

[0044] (5) Cut the MXenes polyethy...

Embodiment 2

[0051] Example 2 Construction of engineered intelligent myocardial tissue based on shad scale base

[0052] A method for constructing an engineered intelligent myocardial tissue on a shad scale base, comprising the following steps:

[0053] (1) Manually tear 3-5 pieces of fish scales from the fresh anchovies, and wash them with water;

[0054] (2) Soak the shad scales in 1 M sodium hydroxide solution for 48 hours; wash the shad scales 4 times with deionized water.

[0055] (3) Soak the shad scales in 0.5 M ethylenediaminetetraacetic acid solution for 72 hours; wash the shad scales with deionized water 4 times and dry them for later use.

[0056] (4), soak the polyethylene film in 0.5% graphene oxide ethanol solution, take out and dry after graphene oxide is adsorbed on the surface of the film to obtain the graphene polyethylene film.

[0057] (5) Cut the graphene polyethylene film the size of fish scale, use liquid PDMS as an adhesive to adhere it to the bottom of the shad s...

Embodiment 3

[0064] Example 3 Construction of engineered intelligent myocardial tissue based on shad scale base

[0065] A method for constructing an engineered intelligent myocardial tissue on a shad scale base, comprising the following steps:

[0066] (1) Manually tear 3-5 pieces of fish scales from the fresh anchovies, and wash them with water;

[0067] (2) Soak the shad scales in 1 M sodium hydroxide solution for 48 hours; wash the shad scales 4 times with deionized water.

[0068] (3) Soak the shad scales in 0.5 M ethylenediaminetetraacetic acid solution for 72 hours; wash the shad scales with deionized water 4 times and dry them for later use.

[0069] (4), soak the polyethylene film in a 1% carbon nanotube ethanol solution, take out the carbon nanotubes after adsorption on the surface of the film, and take it out to dry to obtain a carbon nanotube polyethylene film.

[0070] (5) Cut the carbon nanotube polyethylene film of fish scale size, use liquid PDMS as an adhesive to adhere ...

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Abstract

The invention discloses a method for constructing an engineered intelligent myocardial tissue based on a fish scale substrate. The method comprises the following steps: cleaning fresh fish scales, removing pigments by adopting an alkaline solution, and then carrying out decalcification treatment on the fish scales; a layer of thermally induced deformation film is adhered to the bottom of the fish scale; the treated fish scales are subjected to sterilization and surface modification, and then myocardial cells and other supporting cells are inoculated for co-culture; under the driving of near-infrared laser, the fish scales of the compound cells simulate cardiac contraction to autonomously deform so as to construct intelligent cardiac muscle tissues. According to the method disclosed by the invention, the engineered myocardial tissue with space-time intelligent response can be obtained, the structure and function maturation of the engineered myocardial tissue can be promoted by fully combining directional cell assembly and simulating non-contact mechanical stimulation of an in-vivo physiological environment, and the method is applied to the research of myocardial repair, in-vitro cardiovascular disease model construction and cardiac toxicity screening.

Description

technical field [0001] The invention relates to a method for constructing an engineered intelligent myocardial tissue based on a fish scale base, and belongs to the field of biomedical engineering. Background technique [0002] Cardiovascular disease is currently the leading cause of death worldwide, including in China. The engineered myocardial tissue constructed from human pluripotent stem cell-differentiated cardiomyocytes (hPSC-CMs) provides a new idea for the study of cardiovascular disease, and can be used for cardiovascular disease model construction, cardiac tissue repair and drug cardiotoxicology research. Provide an ideal and reliable platform. However, the current bottleneck is that hPSC-CMs in engineered myocardial tissue are similar to fetal or embryonic cardiomyocytes. The myocardial tissue is immature in structure and function and cannot accurately mimic adult myocardial tissue. Transplantation may also lead to arrhythmia, metabolic disorder, etc. Cardiomyo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/077C12N5/071
CPCC12N5/0657C12N5/069C12N2533/54C12N2533/32C12N2533/90C12N2527/00C12N2535/10
Inventor 肖淼刘青华唐明亮胡士军杜佳
Owner SUZHOU FISHSEEDS BIOLOGICAL TECH CO LTD
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