Electrochemical sensor for ultra-sensitive detection of enrofloxacin and preparation method thereof

A sensitive detection and enrofloxacin technology, applied in the field of electrochemical sensing and detection, can solve the problems of difficult to achieve rapid detection, low sensitivity and high detection limit, and achieve the effects of short detection time, improved sensitivity and low cost

Active Publication Date: 2022-05-27
ZHAOQING UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Aiming at at least one of the problems of the detection method for enrofloxacin in the prior art, such as complex operation, high detection limit, low sensitivity, and difficulty in rapid detection, the present invention provides a low detection limit, good selectivity, simple Fast electrochemical sensor for enrofloxacin

Method used

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  • Electrochemical sensor for ultra-sensitive detection of enrofloxacin and preparation method thereof
  • Electrochemical sensor for ultra-sensitive detection of enrofloxacin and preparation method thereof
  • Electrochemical sensor for ultra-sensitive detection of enrofloxacin and preparation method thereof

Examples

Experimental program
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Embodiment 1

[0034] An electrochemical sensor for ultrasensitive detection of enrofloxacin, the preparation method comprising the following steps:

[0035] (1) The surface of the glassy carbon electrode is ground and polished on the chamois containing 0.05μm alumina slurry, then rinsed with ultrapure water, ethanol and ultrapure water in turn, and dried for use;

[0036] (2) Weigh 2 mg of multi-walled carbon nanotubes and disperse them in 2 mL of 0.1 wt% chitosan solution, ultrasonically disperse for 15 min, and then take 6 μL of the multi-walled carbon nanotube suspension drop and apply it to the surface of the glassy carbon electrode obtained in step (1), drying under an infrared light to obtain a glassy carbon electrode decorated with multi-walled carbon nanotubes;

[0037] (3) After dissolving 1.732 g of p-sulfanilic acid in pure water at 60°C, the volume was adjusted to 100 mL; the multi-walled carbon nanotube-modified glassy carbon electrode obtained in step (2) was placed in the p-s...

experiment example

[0042] Characterize the detection electrodes prepared in Example 1:

[0043] 1. SEM characterization

[0044] The electrodes prepared in Example 1 were characterized by scanning electron microscopy, and the characterization results are shown in the appendix figure 1 , 2 , see appendix figure 1 , the surface of the multi-walled carbon nanotube-modified glassy carbon electrode presents a network-like porous structure formed by staggered multi-walled carbon nanotubes, which significantly increases the electrode surface area and is beneficial to electrolyte ion penetration and electron transfer; see appendix figure 2 , After electropolymerization of p-aminobenzenesulfonic acid, the multi-walled carbon nanotubes on the surface of the glassy carbon electrode are covered with a uniform polymer film, indicating that p-aminobenzenesulfonic acid has been deposited on the carbon nanotube-modified glassy carbon electrode by electropolymerization. surface.

[0045] 2. Electrochemical ...

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Abstract

The invention provides an electrochemical sensor for ultra-sensitive detection of enrofloxacin, and belongs to the technical field of electrochemical sensing detection. A glassy carbon electrode is modified by adopting a poly (p-aminobenzene sulfonic acid) and a multi-walled carbon nanotube composite membrane; through the synergistic effect of the poly (p-aminobenzene sulfonic acid) and the multi-walled carbon nanotubes, the electrochemical sensor disclosed by the invention has high electrochemical response to the enrofloxacin, and high-sensitivity detection of the enrofloxacin is realized. The enrofloxacin electrochemical sensor provided by the invention has the characteristics of low detection limit, wide linear range, high selectivity, good reproducibility and stability and the like, is applied to detection of enrofloxacin residues in an actual sample, is satisfactory in result and has a good application prospect.

Description

technical field [0001] The invention relates to the technical field of electrochemical sensing and detection, in particular to an electrochemical sensor for ultra-sensitive detection of enrofloxacin and a preparation method thereof. Background technique [0002] Enrofloxacin is a third-generation fluoroquinolone antibiotic with broad-spectrum and high-efficiency antibacterial effects. It is widely used in the prevention and treatment of bacterial infectious diseases in poultry, livestock and aquaculture animals. However, excessive use or abuse will lead to excessive residues in animal-derived food, posing a threat to human health. The European Union, Japan and my country all have strict regulations on enrofloxacin residues in animal-derived foods. Therefore, it is necessary to accurately and sensitively detect enrofloxacin residues in animal-derived foods. [0003] The current methods for the analysis and detection of enrofloxacin include high performance liquid chromatogr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/30G01N27/327G01N27/48B24B1/00
CPCG01N27/308G01N27/327G01N27/48B24B1/00Y02A50/30
Inventor 韦寿莲谢春生黄象金蔡秋燕
Owner ZHAOQING UNIV
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