Western blot novel efficient protease inhibitor

Abstract

The invention discloses a novel efficient protease inhibitor for western blot. The novel efficient protease inhibitor for western blot is characterized by comprising lepileutin, an enzyme inhibition peptide, AEBSF, a pepsin inhibitor, E-64 and Beastatin which are prepared in DMSO (dimethylsulfoxide). The volume of the prepared DMSO solution is 1ml. The molar mass of the lepidin is 15 to 20 [mu] mol, the molar mass of the enzyme inhibition peptide is 0.7 to 0.9 [mu] mol, the molar mass of the pepsin inhibitor is 10 to 11 [mu] mol, the molar mass of the E-64 is 14 to 15 [mu] mol, and the molar mass of the Beastatin is 46 to 52 [mu] mol. In the implementation process, the formula of the protease inhibitor can be improved and optimized, the inhibition effect is more efficient, the proportion is optimized according to a large amount of experimental data, the mixed solution is prepared, and the experimental process is simplified and stabilized.

Description

Technical field [0001] The present invention belongs to the field of molecular biotechnology technology, specifically a western blot novel highly efficient protease inhibitor. Background [0002] Western blotting (western blotting test) is Western Blot. It is an experimental method commonly used in molecular biology, biochemistry and immunogenetics. The basic principle is to color cells or biological tissue samples treated by gel electrophoresis by specific antibodies. Information is obtained by analyzing the location and depth of coloring of a particular protein in the analyzed cell or tissue. Cell or tissue extracts contain many endogenous proteases, phosphatase, etc., which can easily lead to protein degradation or demodification in the extract, thus affecting subsequent protein detection. Therefore, adding appropriate protease, phosphatase and other inhibitors to the extract is an effective way to prevent protein degradation and demodification. Contents of the Invention [0...

AI Technical Summary

Problems solved by technology

Cell or tissue extracts contain many endogenous proteases, phosphatases, etc., which can easily lead to protein degradation or demodification in the extract, thus affecting subsequent protein detection