Immunochromatography test strip based on nano-fiber membrane with gradient pore size distribution and preparation method of immunochromatography test strip

A technology of immunochromatography test paper and nanofiber membrane, which is applied in the field of immunochromatography, can solve the problems of increasing the capillary flow time of the specific surface area, and achieve the effects of delaying the time of immune reaction, good protein adsorption performance, and convenient operation

Pending Publication Date: 2022-05-27
DONGHUA UNIV
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But usually, these methods only design and debug the entire chromatographic membrane from a single point of view such as reducing the pore size to increase the specific surface area or increasing the hydrophobicity to reduce the capillary flow time, which inevitably sacrifices a certain amount of chromatographic time to achieve More sensitive detection effect

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Immunochromatography test strip based on nano-fiber membrane with gradient pore size distribution and preparation method of immunochromatography test strip
  • Immunochromatography test strip based on nano-fiber membrane with gradient pore size distribution and preparation method of immunochromatography test strip
  • Immunochromatography test strip based on nano-fiber membrane with gradient pore size distribution and preparation method of immunochromatography test strip

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] A preparation method of an immunochromatographic test strip based on a pore size gradient distribution nanofiber membrane, the specific steps are as follows:

[0065] (1) Spinning solution A (trichloromethane solution of polycaprolactone) with a concentration of 15 wt % and spinning solution B (isopropanol and water of polyvinyl alcohol ethylene copolymer with a concentration of 6 wt %) (volume ratio 7 :3) solution) is placed on the syringe pump in the electrospinning machine, and connected to the 20 gauge stainless steel needles on both sides of the receiver, the needles are fixed, the drum is rotated, and the spinning solution A is electrospun first. A large-pore nanofiber membrane was obtained. After the film was deposited to a thickness of 150 μm, the syringe pump that controlled the spinning solution A was turned off, the needle was moved to the right by 50 mm and the spinning parameters were adjusted, and the syringe pump that controlled the spinning solution B was...

Embodiment 2

[0077] A preparation method of an immunochromatographic test strip based on a pore size gradient distribution nanofiber membrane, the specific steps are as follows:

[0078] (1) A spinning solution A (polylactic acid in tetrahydrofuran solution) with a concentration of 15 wt % and a spinning solution B (N,N-dimethylformamide and tetrahydrofuran in carboxylated polyvinyl chloride (carboxylated polyvinyl chloride) with a concentration of 13 wt % (volume The ratio is 3:2) solution) is placed on the syringe pump in the electrospinning machine, and connected to the 19 gauge stainless steel needles on both sides of the receiver, the needles are fixed, rotate the drum, and spin the spinning solution A first. Electrospinning obtains a nanofiber membrane with large pore size. After the film is deposited to a thickness of 120 μm, the syringe pump that controls the spinning solution A is turned off, the needle is moved to the right by 40 mm and the spinning parameters are adjusted, and th...

Embodiment 3

[0090] A preparation method of an immunochromatographic test strip based on a pore size gradient distribution nanofiber membrane, the specific steps are as follows:

[0091] (1) A spinning solution A (polycaprolactone in chloroform solution) with a concentration of 15 wt% and a spinning solution B (N,N-dimethylacetamide and acetone in cellulose acetate in a concentration of 15 wt%) (volume ratio 2:3) solution) is placed on the syringe pump in the electrospinning machine, and connected to the 20-gauge stainless steel needles on both sides of the receiver, the needles are fixed, rotate the drum, first spin solution A Electrospinning was carried out to obtain a large-pore nanofiber membrane. After the film was deposited to a thickness of 110 μm, the syringe pump that controlled the spinning solution A was turned off, the needle was moved to the right by 40 mm and the spinning parameters were adjusted, and the syringe pump that controlled the spinning solution B was turned on. Mak...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Apertureaaaaaaaaaa
Apertureaaaaaaaaaa
Thicknessaaaaaaaaaa
Login to view more

Abstract

The invention relates to an immunochromatography test strip based on a nanofiber membrane with gradient pore size distribution and a preparation method of the immunochromatography test strip. The immunochromatography test strip comprises a bottom plate as well as a sample pad, a combination pad, a chromatography membrane and a water absorption pad which are attached to the bottom plate and are sequentially arranged in an overlapped manner, the chromatographic membrane is a cross-linked nanofiber membrane with gradient pore size distribution (the pore size of a large-pore-size nanofiber membrane is 2-3.6 mu m and the porosity is 60-80%, and the pore size of a small-pore-size nanofiber membrane is 0.8-2 mu m and the porosity is 70-90%); the preparation method comprises the following steps: carrying out cross-linking treatment on the pore size gradient distribution nanofiber membrane through a protein cross-linking agent to obtain a chromatographic membrane, and sequentially attaching the sample pad, the combination pad and the water absorption pad to the bottom plate attached with the chromatographic membrane to prepare the immunochromatographic test strip based on the pore size gradient distribution nanofiber membrane. According to the invention, the combination of large and small apertures of the aperture gradient nanofiber membrane is utilized, so that the protein adsorption capacity is greatly improved, the immunoreaction time is delayed, and the whole test paper detection speed is not influenced.

Description

technical field [0001] The invention belongs to the technical field of immunochromatography, and relates to an immunochromatography test strip based on a pore size gradient distribution nanofiber membrane and a preparation method thereof. Background technique [0002] The immunochromatographic test strip is a simple and easy-to-use rapid detection technology developed in the 1980s. Tracer markers, using the principle of specific binding of antigens and antibodies in immunology, when the liquid sample solution is subjected to capillary action flow chromatography, a series of specific Combined, the colored immune complexes finally accumulate in the capture area on the chromatographic membrane, so that the color change can be observed to achieve the purpose of detection. Compared with electrochemical analysis, enzyme-linked immunosorbent assay, microbial detection and other methods, this technology has been widely used in the field of biomedical detection due to its advantages...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N33/558G01N33/543G01N33/58D04H1/728
CPCG01N33/558G01N33/54306G01N33/54393G01N33/587G01N33/9446D04H1/728
Inventor 李彦郁娟张莹洁高锦明王富军王璐
Owner DONGHUA UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap