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Method for promoting enrichment of anaerobic ammonium oxidation bacteria by nitrogen-doped graphene

A nitrogen-doped graphene, anaerobic ammonium oxidizing bacteria technology, applied in anaerobic digestion treatment, chemical instruments and methods, water pollutants, etc. Long doubling time of bacteria, etc., to achieve the effect of promoting metabolic function, good sedimentation performance, and increasing abundance

Pending Publication Date: 2022-06-03
QILU UNIV OF TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the long doubling time of anammox bacteria (10-12d) and sensitivity to changes in the external environment, it is difficult to achieve large-scale application of the sewage treatment process based on anammox.
For sewage treatment plants, taking too long to cultivate anammox sludge will undoubtedly increase the cost of starting and running the reaction system, wasting a lot of manpower and material resources

Method used

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  • Method for promoting enrichment of anaerobic ammonium oxidation bacteria by nitrogen-doped graphene
  • Method for promoting enrichment of anaerobic ammonium oxidation bacteria by nitrogen-doped graphene
  • Method for promoting enrichment of anaerobic ammonium oxidation bacteria by nitrogen-doped graphene

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] The preparation method of nitrogen-doped graphene comprises the following steps:

[0052] (1) prepare graphene oxide according to the improved Hummers method, specifically comprise the steps:

[0053] (1) Dissolve 5g of flake graphite and 2.5g of sodium nitrate in 120ml of concentrated sulfuric acid (98wt%), slowly add 15g of potassium permanganate in an ice-water bath, and stir and mix for 2 hours to prepare mixture I.

[0054] (2) The mixture I obtained in step (1) was stirred in an oil bath at 35°C for 2 hours, then 200 ml of deionized water was added, and the reaction was continued to stir in an oil bath at 98°C for 1.5 hours to prepare mixture II.

[0055] (3) Add mixture II obtained in step (2) to deionized water until the total volume reaches 1000 ml to prepare mixture III, then add 20 ml of hydrogen peroxide, seal and place for 12 hours to prepare mixture.

[0056] (4) After discarding the supernatant of the mixture in step (3), the obtained mixed system was ul...

Embodiment 2

[0063] Two anaerobic reactors (2L) with the same specifications were constructed. R1 only contained sludge as a blank group, and R2 contained a mixture of sludge and nitrogen-doped graphene as an experimental group. The sludge used in the experiment is the mature sludge from the laboratory-scale up-flow anaerobic sludge bed reactor. The volatile suspended solid concentration of the inoculated sludge is about 1774 mg / L, and the volume ratio of the inoculated sludge to the wastewater is 1:4. The final concentration of nitrogen-doped graphene dosage was 50 mg / L, and it was added at one time. In this experiment, the inoculated sludge was continuously cultured with a period of 110 d, the reactor was operated in the dark, the temperature was controlled at 34±1 °C, and the wastewater was mainly composed of NH 4 + -N and NO 2 - -N composition, supplemented by growth elements required for microbial growth and the pH of the wastewater is regulated at 7.5 ± 0.2, the hydraulic retenti...

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Abstract

The invention provides a method for promoting enrichment of anaerobic ammonium oxidation bacteria by nitrogen-doped graphene, and particularly relates to application of nitrogen-doped graphene in promotion of enrichment of anaerobic ammonium oxidation bacteria and denitrification of wastewater, sludge and nitrogen-doped graphene are inoculated in wastewater, anaerobic ammonium oxidation sludge is cultured by controlling operation conditions, and denitrification of the wastewater is realized; the nitrogen-doped graphene not only promotes the growth of specific microorganisms participating in anaerobic ammonium oxidation, but also improves the key functions of anaerobic ammonium oxidation flora and the abundance of genes, and the effects jointly promote the realization of higher nitrogen removal efficiency of the reactor.

Description

technical field [0001] The invention belongs to the technical field of wastewater treatment, and in particular relates to a method for efficiently denitrifying nitrogen from wastewater by promoting the enrichment of anammox bacteria and up-regulating key functional genes of the flora by nitrogen-doped graphene. technical background [0002] Compared with the traditional nitrification-denitrification process, anammox can reduce 50% aeration amount, 100% organic carbon source and 90% operating cost, and is a more energy-saving and efficient autotrophic denitrification technology. However, due to the long doubling time (10-12 days) of anammox bacteria and their sensitivity to changes in the external environment, it is difficult to achieve large-scale application of the wastewater treatment process based on anammox. For sewage treatment plants, spending too long to cultivate anammox sludge will undoubtedly increase the cost of starting the reaction system and waste a lot of manp...

Claims

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Application Information

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IPC IPC(8): C02F3/28C02F101/16
CPCC02F3/28C02F2101/16Y02W10/10
Inventor 薛嵘张宝永郭艳李振燕锡尧张冠军杨文娟
Owner QILU UNIV OF TECH